Polyvalent antibody and preparation method thereof

A multivalent antibody and single-chain antibody technology, applied in chemical instruments and methods, anti-enzyme immunoglobulin, hybrid immunoglobulin, etc., can solve the problems that cannot be well promoted and applied, and achieve low treatment costs and eliminate pathogens Dangerous Effects of Infection

Inactive Publication Date: 2020-01-17
深圳容金科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0010] In view of the above technical problems, the embodiment of the present invention provides a multivalent antibody and its preparation method to solve the problems of a series of defects in the existing tumor immunotherapy methods, which cannot be well promoted and applied

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  • Polyvalent antibody and preparation method thereof
  • Polyvalent antibody and preparation method thereof
  • Polyvalent antibody and preparation method thereof

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preparation example Construction

[0058] The embodiment of the present invention also provides a preparation method for preparing the above-mentioned multivalent antibody. image 3 The preparation method of the multivalent antibody provided by the embodiment of the present invention. Such as image 3 Shown, described preparation method comprises:

[0059] S110. Prepare monoclonal antibodies against different antigens. The monoclonal antibody contains an antigen-binding site that specifically binds to the antigen.

[0060] The specific preparation process of monoclonal antibodies is well known to those skilled in the art, and will not be repeated here. In some embodiments, the improved monoclonal antibody screening technology can be used to shorten the preparation time and simplify the preparation process, and the monoclonal antibody gene to be prepared can be transfected into the co-expression cells of the screening system. Whether there is antibody expression is determined by whether the cells express gre...

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Abstract

The embodiment of the invention discloses a polyvalent antibody and a preparation method thereof. The polyvalent antibody includes a plurality of antigen-binding sites, wherein the antigen-binding sites are connected to a human-source antibody core fragment; and the antigen-binding sites at least include antigen-binding sites specifically bound with tumor cells and antigen-binding sites specifically bound with immunocyte. The polyvalent antibody is obtained through reformation in a genetic engineering manner, and can enable the immunocyte to be bound to the cancer cells to arouse attack of theimmunocyte on the cancer cells. Compared with a conventional CAR-T treatment method, a treatment method disclosed by the invention has the advantages that the polyvalent antibody has broad spectrum,does not depend on autologous T cells of patients, and can be in mass production. In addition, waiting time does not exist, and the polyvalent antibody can be immediately prepared for use. Relying ona mature antibody production technology, risk infected by other pathogens can be eliminated in the manner of GMP and the like, and the polyvalent antibody has lower treatment fees.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a multivalent antibody and a preparation method thereof. Background technique [0002] Traditional treatments for cancer include surgery, radiation, chemotherapy, and targeted therapy. However, these traditional treatment methods have limitations to varying degrees, and can only delay but cannot fundamentally prevent the development of the disease. [0003] For example, surgical treatment is only suitable for tumor tissues that are visible to the naked eye. Radiotherapy and chemotherapy can kill a large number of tumor cells, but they are not specific enough and will destroy a large number of normal tissue cells and produce strong side effects. Although targeted drugs have obvious therapeutic effects and quick results, they are prone to drug resistance and heterogeneity of tumor cells, and cannot exert long-term anti-tumor effects. [0004] In order to overcome the limitations of t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/46C07K19/00
CPCC07K14/55C07K16/2803C07K16/2809C07K16/2818C07K16/2863C07K16/32C07K16/40C07K2317/31C07K2317/622C07K2317/73C07K2317/92
Inventor 邓兴朝陈欢
Owner 深圳容金科技有限公司
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