Preparation method and application of trivalent live vaccine for chicken coccidiosis

The technology of chicken coccidiosis and live vaccine is applied in the field of preparation of chicken coccidiosis trivalent live vaccine, which can solve the problems of intestinal mucosal epithelial damage, poor intestinal digestion, poor absorption, and decreased feed conversion rate, etc. The effect of less stress, good immunogenicity and low cost

Inactive Publication Date: 2020-01-10
TIANJIN HLINTE BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Chicken coccidiosis, as a common disease that harms the chicken industry, causes economic losses of several billion dollars in the world every year. It is parasitic by one or several Eimeria coccidiosis in chicken intestinal epithelial cells, and infects many chickens. Intestinal mucosal epithelium is damaged, intestinal digestion and absorption are poor, and even fatal, with a mortality rate as high as 80%. The cost of prevention and control continues to increase, the emergence of drug-resistant strains and the impact of drug residues on the quality of poultry and human health have occurred, and the feed conversion rate of chickens has also dropped significantly. Short storage time, unchanged transportation and other disadvantages, the coccidia vaccine weakened by chemical reagents will be more stressful, so the development of a physically or biologically attenuated live coccidia vaccine will become a research hotspot in the broiler chicken farming industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] A preparation method and application of a chicken coccidiosis trivalent live vaccine. The steps of the preparation method are:

[0019] (1) Early maturity breeding for 3 to 30 generations of Eimeria tenella, Eimeria toxic and Eimeria giant three coccidia, and continuously pathogenic strains for each breeding Evaluation is performed until it meets the requirements of vaccine strains with low pathogenicity, high immunogenicity, and high cross-protection rate.

[0020] (2) Count the sporulated oocysts of Eimeria tenella, Eimeria poisonous and Eimeria giant with a hemocytometer, according to 600 Eimeria tenella per head Calculate the number of coccidia oocysts, put it in a clean container, and add 1% chloramine T to poison 700 Eimeria per head, and 200 Eimeria giant per head. The preservation solution of the solution is stirred to obtain the mixed insect strain oocyst solution of the corresponding component quantity, and it is stored in an environment at 2-8°C for later use.

Embodiment 2

[0022] A preparation method and application of a chicken coccidiosis trivalent live vaccine. The steps of the preparation method are:

[0023] (1) Early maturity breeding for 3 to 30 generations of Eimeria tenella, Eimeria toxic and Eimeria giant three coccidia, and continuously pathogenic strains for each breeding Evaluation is performed until it meets the requirements of vaccine strains with low pathogenicity, high immunogenicity, and high cross-protection rate.

[0024] (2) Count the sporulated oocysts of Eimeria tenella, Eimeria poisonous and Eimeria giant with a hemocytometer, and count 1200 Eimeria tenella per head. Calculate the number of coccidian oocysts, put it in a clean container, and add 5% chloramine T to poison Eimeria 1500 / head, and 800 giant Eimeria / head. The preservation solution of the solution is stirred to obtain the mixed insect strain oocyst solution of the corresponding component quantity, and it is stored in an environment at 2-8°C for later use.

Embodiment 3

[0026] A preparation method and application of a chicken coccidiosis trivalent live vaccine. The steps of the preparation method are:

[0027] (1) Early maturity breeding for 3 to 30 generations of Eimeria tenella, Eimeria toxic and Eimeria giant three coccidia, and continuously pathogenic strains for each breeding Evaluation is performed until it meets the requirements of vaccine strains with low pathogenicity, high immunogenicity, and high cross-protection rate.

[0028] (2) Count the sporulated oocysts of Eimeria tenella, Eimeria poisonous and Eimeria giant with a hemocytometer, according to 900 Eimeria tenella per head Calculate the number of coccidia oocysts, put it in a clean container, and add 2% chloramine T to poison Eimeria 1200 / head, and giant Eimeria 300 / head. The preservation solution of the solution is stirred to obtain the mixed insect strain oocyst solution of the corresponding component quantity, and it is stored in an environment at 2-8°C for later use.

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Abstract

The invention relates to a preparation method and application of a trivalent live vaccine for chicken coccidiosis. The vaccine is prepared by uniformly mixing early-maturing breeding attenuated strains of eimeria tenella, eimeria necatrix and eimeria maxima, and storing the obtained mixture in a preservation solution. The invention further relates to a preparation method and application of the trivalent live vaccine for chicken coccidiosis. The vaccine adopts physical attenuation, free from drug residues and drug resistance, good in immunogenicity, safe, free from stress and capable of being used for preventing chicken coccidiosis in large-scale farms.

Description

Technical field [0001] The invention belongs to the field of veterinary biological products, in particular to a preparation method and application of a trivalent live vaccine for chicken coccidiosis. Background technique [0002] Chicken coccidiosis is a common disease that harms the chicken industry, causing hundreds of millions of dollars in economic losses worldwide every year. It is caused by one or several Eimeria parasites in the intestinal epithelial cells of chickens, which infect chickens. Intestinal mucosal epithelium is damaged, intestinal digestion and absorption are poor, and even fatal. The mortality rate is as high as 80%. At present, anti-coccidiosis drugs and virulent coccidiosis vaccines are mainly used to prevent them, but with coccidiosis drugs The continuous increase in control costs, the emergence of drug-resistant strains and the effects of drug residues on the quality of meat and poultry and human health have emerged. The feed conversion rate of the chicke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/012A61P33/02A61P1/00
CPCA61K39/012A61K2039/522A61K2039/552A61P1/00A61P33/02
Inventor 陈庆忠安同伟李迎梅
Owner TIANJIN HLINTE BIOTECH CO LTD
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