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Short-cut nitrification and denitrification Acinetobacter variabilis strain and application thereof

A short-range nitrification and denitrification technology, applied in bacteria, chemical instruments and methods, biochemical equipment and methods, etc., can solve problems such as exceeding the standard, and achieve the effect of good denitrification capacity.

Active Publication Date: 2020-01-07
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] In view of this, the purpose of the present invention is to provide a strain of Acinetobacter mutans strain with short-range nitrification and denitrification ability, which has good application for solving the eutrophication problems such as ammonia nitrogen, nitrite and nitrate nitrogen exceeding the standard in aquaculture water prospect

Method used

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  • Short-cut nitrification and denitrification Acinetobacter variabilis strain and application thereof
  • Short-cut nitrification and denitrification Acinetobacter variabilis strain and application thereof
  • Short-cut nitrification and denitrification Acinetobacter variabilis strain and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Acinetobacter mutans acquisition

[0038] 1. Prepare the following medium:

[0039] (1) Liquid seed liquid medium: beef extract 5g / L, peptone 10g / L, NaCl 5g / L, H 2 O1L, sterilized at 121°C for 25 minutes.

[0040] (2) Nitrification medium (BM): K 2 HPO 4 7g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O 0.1g, (HN 4 ) 2 SO 4 0.707g, FeSO 4 ·7H 2 O 0.05g, CH 3 COONa 7.68g, H 2 O 1L. Sterilize at 121°C for 25 minutes.

[0041] (3) Denitrification medium 1 (DM-1): K 2 HPO 4 7g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O0.1g, NaNO 2 0.739g, FeSO 4 ·7H 2 O 0.05g, CH 3 COONa 7.68g, H 2 O 1L. Sterilize at 121°C for 25 minutes.

[0042] (4) Denitrification medium 2 (DM-2): K 2 HPO 4 7g, KH 2 PO 4 3g, MgSO 4 ·7H 2 O0.1g, KNO 3 1.07g, FeSO 4 ·7H 2 O 0.05g, CH 3 COONa 7.68g, H 2 O 1L. Sterilize at 121°C for 25 minutes.

[0043] 2. Screening of heterotrophic nitrifying bacteria

[0044] (1) Sample collection: It was taken from the bottom mud of the pond in the aquaculture...

Embodiment 2

[0050] Identification of strains

[0051] 1. Identification of strain morphological characteristics

[0052] The morphological characteristics of its colony on the beef extract peptone solid medium are: the colony is yellow-white, round, raised, with neat edges, moist surface, and opaque.

[0053] 2. Physiological and biochemical characteristics of strain DW-10

[0054] The physiological and biochemical indicators of strain DW-10 were determined according to the method provided by "Microbiological Experiment" (fourth edition) (Shen Ping, edited by Chen Xiangdong, 111-117). Table 1 is the physiological and biochemical characteristics of strain DW-10.

[0055] Table 1 Physiological and biochemical characteristics of strain DW-1

[0056]

[0057] Note: "+" stands for positive, "-" stands for negative; "+" stands for positive and "-" stands for negative

[0058] 3. Molecular biological identification of strains

[0059] 1) Genomic DNA of strain DW-10 was extracted using th...

Embodiment 3

[0067] Application of Strain DW-10 in Nitrogen Removal from Freshwater Aquaculture Wastewater

[0068] What this embodiment adopts is intensive culture pond aquaculture wastewater, and the aquaculture mode is polyculture, wherein grass carp is 25%, silver carp and bighead carp 60%, and the rest is crucian carp and bream. The water quality of this aquaculture wastewater is: total nitrogen 2.94mg / L , ammonia nitrogen 1.27mg / L, nitrite nitrogen 0.25mg / L, nitrate nitrogen 0.23mg / L, pH value around 7.3. The fermented liquid of above-mentioned bacterial strain DW-10 (containing bacteria number is 10 12 CFU / mL) was inoculated with 2% of the inoculum amount into intensive culture pond culture wastewater adjusted to pH 7.2, at a temperature of 25°C, a C / N ratio of 10, and a shaker speed of 100-130r / min, cultured for 24 hours, and measured treatment The content changes of total nitrogen, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen in aquaculture wastewater before and after....

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Abstract

The invention provides a short-cut nitrification and denitrification Acinetobacter variabilis strain and application thereof, and belongs to the technical field of functional microorganisms. The short-cut nitrification and denitrification Acinetobacter variabilis strain DW-10 is preserved under the preservation number CCTCC NO: M2019167. The invention further discloses a rapid denitrification microbial agent. The rapid denitrification microbial agent comprises the short-cut nitrification and denitrification Acinetobacter variabilis strain DW-10 as an active ingredient. The short-cut nitrification and denitrification Acinetobacter variabilis strain DW-10 can grow, under aerobic conditions, by taking organic carbon as a carbon source, as well as ammonia nitrogen, nitrate nitrogen or nitritenitrogen as the sole nitrogen source; and heterotrophic nitrification and aerobic denitrification are synchronously carried out. The short-cut nitrification and denitrification Acinetobacter variabilis strain DW-10 provided by the invention has high-efficiency removal capacity for total nitrogen, ammonia nitrogen, nitrate nitrogen and nitrite nitrogen; and thus, a green, safe and pollution-free biological pathway is provided for water quality improvement of freshwater aquaculture.

Description

technical field [0001] The invention belongs to the technical field of functional microorganisms, and in particular relates to a short-range nitrification and denitrification variant Acinetobacter strain and its application. Background technique [0002] At present, biological denitrification is one of the most widely used sewage denitrification technologies at home and abroad. The short-cut nitrification and denitrification biological denitrification process is a new type of biological denitrification technology. This technology controls the conversion of nitrogen by changing the denitrification conditions. , the nitrification process stays in the nitrosation stage, forming NO 2 -N accumulates in large quantities, and then directly reduces nitrite nitrogen to N by denitrification 2 . [0003] In recent years, due to the increasingly serious problem of eutrophication in freshwater aquaculture, the traditional intensive farming method itself inevitably pollutes the surround...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/16
CPCC02F3/34C02F2101/16C02F2101/163C02F2101/166C12N1/205C12R2001/01
Inventor 兰时乐杜全能朱文娟胡超肖思远陈思宇
Owner HUNAN AGRICULTURAL UNIV
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