Simultaneous and rapid determination method of vitamin A, vitamin D3 and vitamin E

Abstract

The present invention belongs to the technical field of analysis and detection, in particular to a simultaneous and rapid determination method of vitamin A, vitamin D<3> and vitamin E. The method comprises the following steps: (1) vitamin A, vitamin D<3> and vitamin E in a sample to be tested are extracted with dimethyl sulfoxide; and the obtained extraction liquid is oscillated and extracted withn-hexane, concentrated and redissolved to obtain a sample solution; (2) standard solutions of vitamin A, vitamin D<3> and vitamin E are prepared; and multiple standard series of solutions are prepared according to the concentration gradient; (3) the sample solution and the standard solutions are detected and analyzed with the high performance liquid chromatography; and the contents of vitamin A,vitamin D<3> and vitamin E are determined with an external standard method. With the method, simultaneous determination of vitamin A, vitamin D<3> and vitamin E is realized; the pretreatment time of the sample is greatly shortened; the work efficiency is improved; at the same time, the detection accuracy is improved; and the laboratory cost is reduced.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, in particular to a method for synchronous and rapid detection of vitamin A and vitamin D 3 and vitamin E methods. Background technique [0002] Vitamins A, D and E are fat-soluble vitamins, and fat-soluble vitamins are polypentadienyl compounds composed of long hydrocarbon chains or fused rings. Vitamin A (vitamin A acetate), vitamin D 3 (cholecalciferol) and vitamin E (vitamin E acetate) are essential for human health, among them, vitamin D 3 It is an important nutritional ingredient for mammals, and it can promote calcium and phosphorus metabolism and bone formation in mammals. Therefore, vitamin A and vitamin D 3 Vitamin E and vitamin E are the main nutritional components of infant milk powder and rice flour, and are also important components of various health foods. [0003] Vitamin A and vitamin E have strong ultraviolet absorption and high content in food. One-dimensional...

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Problems solved by technology

[0005] The purpose of the present invention is to solve the problem that vitamin A and vitamin D cannot be produced simultaneously in the prior art. 3 and vitamin E content detection, a simultaneous and rapid detection of vitamin A and vitamin D was proposed 3 and vitamin E method, can achieve vitamin A, D 3 , E synchronous detection, can greatly shorten the sample pretreatment time, improve work efficiency, improve detection accuracy and reduce laboratory costs

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