Method for identifying resistance of epidemic diseases of capsicum annuum at seedling stage by root-dipping and inoculating
A technology of blight resistance and identification method, which is applied in the field of root dip inoculation identification of blight resistance in pepper seedling stage, and can solve the problems such as heavy workload for propagating Phytophthora, unsuitable screening of a large number of materials, inconvenience in screening large-scale anti-blight breeding materials, etc. , to achieve the effect of simple and rapid screening, solving time-consuming and labor-intensive problems, and easy to operate and master
Pending Publication Date: 2019-12-31
黑龙江省农业科学院园艺分院
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Root irrigation method is currently the most commonly used method for identification of pepper blight, but this method requires a large number of spores, and the existing solid medium is used to cultivate Phytophthora, the workload of mass propagation of Phytophthora is too large, and it is not suitable for screening a large number of materials. It brings inconvenience to the screening of large-scale anti-epidemic breeding materials
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[0032] A root soaking and inoculation identification method for capsicum seedling blight resistance, the method is carried out as follows:
[0033] Preparation of pepper seedlings:
[0034] 35 pepper varieties were selected (Table 1). The above-mentioned varieties are obtained through commercial means or scientific research means (introduced in the seed resource bank), but the present invention is not limited to the above-mentioned varieties.
[0035] Disinfect the pepper seedlings before sowing, select plump seeds, soak the seeds in warm water at 52-55°C for 30 minutes, stir while soaking, soak in 10% sodium hypochlorite solution for 5 minutes, stir while soaking, rinse with clean water and soak in clean water for 48 hours , Take the seeds out of clear water, wrap them in gauze to moisturize and accelerate germination, and after the seeds are white, sow a single seed in a plug filled with a seedling substrate. The substrate for raising seedlings is vermiculite, peat and soi...
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The invention belongs to the technical field of disease resistance identification of horticultural crops and particularly relates to a method for identifying the resistance of epidemic diseases of capsicum annuum at the seedling stage by root dipping and inoculating. The method comprises the steps of capsicum annuum seedling preparation, spore suspension preparation, inoculation, disease investigation, evaluation and the like, wherein the bared root and caudex parts of the capsicum annuum seedlings are soaked with the spore suspension having a concentration of 10 zoospores / mL when the seedlingage is 5-6-leaf stage; the disease index is investigated on the fifth day after inoculation, and grading is performed according to the disease index, and the resistance of varieties is evaluated. Themethod can identify the resistance by utilizing a root-dipping and inoculating method, has the advantage of low inoculation concentration, can solve the problems of time and labor consumption for culturing mass bacteria, is in accordance with natural incidence situation since bacteria contact with the root part, is standard and scientific in disease index grading, has low requirement for the inoculation environment, is easy to use, and provides technical support to screening of large-scale capsicum annuum disease-resistant breeding materials.
Description
technical field [0001] The invention relates to the technical field of plant disease identification, in particular to a root soaking inoculation identification method for pepper seedling blight resistance. Background technique [0002] Pepper blight is becoming more and more serious in our country, and has become one of the main diseases affecting pepper production. The disease is caused by Phytophthora capsici L., which uses sporangia to overwinter on soil and field diseased residues, and spreads the disease by means of rainwater or irrigation water. Pepper blight is caused by zoospores infecting the roots. The disease occurs at the seedling stage, and the disease usually starts from the base of the stem, and water-soaked soft rot appears on the diseased part, and the diseased part is lodging above the diseased part. Infected after planting, leaves soft rot and fall off, dark green lesions appear on stems, water-soaked irregular lesions occur on fruits, and flesh rots sof...
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IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 赫卫张慧
Owner 黑龙江省农业科学院园艺分院
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