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Method for improving production and transformation efficiency of erythrite and application of method

A technology of erythritol and conversion efficiency, which is applied in the field of biological fermentation, can solve the problems of low conversion rate and no practical use value, and achieve the effect of increased conversion rate, low cost and simple operation

Active Publication Date: 2019-12-31
BINZHOU SANYUAN BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The U.S. invention patent US6110715 discloses a method of using Trichosporonoides megachiliensis yeast to ferment glucose to synthesize erythritol. Although this bacterial strain meets my country’s national standards, the highest conversion rate reported is only 35.1%. The same conversion rate is low and has no practical use value.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] The present embodiment provides a method for improving the conversion efficiency of erythritol production, the method is as follows:

[0053] (1) Treat the fermentation medium at a temperature of 80°C for 40 minutes. After cooling, inoculate the strain of Y. lipolytica in the fermentation medium for cultivation. The initial temperature is 30°C and the initial pH value is 6.0. When the density OD600 value was 20, adjust the pH value to 3.7; the formula of the fermentation medium was: starch hydrolyzate 300g / L, diammonium hydrogen phosphate 5g / L, yeast powder 15g / L, corn steep liquor dry powder 15g / L, Magnesium sulfate 0.25g / L, manganese sulfate 0.25g / L, manganese chloride 0.25g / L, copper sulfate 0.25g / L, copper chloride 0.25g / L, zinc sulfate 0.25g / L, zinc chloride 0.25g / L.

[0054] (2) After secondary expansion cultivation, transfer to the production fermentation medium to carry out the conversion of erythritol. The production fermentation medium is the same as the fer...

Embodiment 2

[0057] The present embodiment provides a method for improving the conversion efficiency of erythritol production, the method is as follows:

[0058] (1) Treat the fermentation medium at a temperature of 90°C for 20 minutes. After cooling, inoculate the Y. lipolytica strain into the fermentation medium for cultivation. The initial temperature is 25°C and the initial pH value is 5.0. When the density OD600 value is 20, adjust the pH value to be 3.1; the formula of the fermentation medium is: starch hydrolyzate 200g / L, diammonium hydrogen phosphate 15g / L, yeast powder 2g / L, corn steep liquor dry powder 20g / L, Magnesium sulfate 0.10g / L, manganese sulfate 0.5g / L, manganese chloride 0.10g / L, copper sulfate 0.10g / L, copper chloride 1.0g / L, zinc sulfate 0.25g / L, zinc chloride 0.25g / L.

[0059] (2) After secondary expansion cultivation, transfer to the production fermentation medium to carry out the conversion of erythritol. The production fermentation medium is the same as the ferme...

Embodiment 3

[0062] This embodiment provides a method for improving the conversion efficiency of erythritol production. The difference between the method and Example 2 is that the concentration of cyclomaltodextrin-glucanotransferase in step (2) is 50000 U / L. Other conditions remain unchanged. The conversion rate of erythritol was 61.1%.

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Abstract

The invention relates to a method for improving production and transformation efficiency of erythrite and an application of the method. The method comprises the steps that glucose is used as a raw material, yeast is used as a fermentation and transformation bacterial strain, and in the fermentation and conversion process, sucrose synthase and malt cyclodextrin-glucan transferase are added, so thatthe conversion rate of glucose into erythrite can be increased. The method is simple to operate and low in cost, and the conversion rate is notably increased.

Description

technical field [0001] The invention belongs to the technical field of biological fermentation, in particular relates to a method for preparing erythritol and its application, in particular to a method for improving the production conversion efficiency of erythritol and its application. Background technique [0002] Erythritol (also known as butylene glycol) is a tetravalent polyol with a molecular weight of 122.1Da. It exists in many fruits, vegetables and brewed foods (such as pears, glucose and other fruits and wine), but the content is very small . Because erythritol has the advantages of zero calories, no dose limit, and pure biological fermentation preparation, erythritol is more widely used in food, and has been widely used in candies, baked goods, sugar-free drinks and other foods. For example: the invention patent with application number 200710014055.8 discloses a method for making sandwich chocolate containing erythritol; the invention patent with application numb...

Claims

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Application Information

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IPC IPC(8): C12P7/18C12R1/73C12R1/645
CPCC12P7/18
Inventor 李丽聂在建程保华李德春
Owner BINZHOU SANYUAN BIOLOGICAL TECH
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