Study on the extraction process and antioxidant properties of a kind of ginger flavone

A technology of turmeric and extraction process, which is applied in the directions of antitoxin, color/spectral property measurement, medical preparations containing active ingredients, etc., to achieve the effect of good antioxidant performance and effective utilization

Active Publication Date: 2021-11-23
ANHUI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few patents related to the extraction of flavonoids in ginger. Patent CN109364520A (Cheng Yan, a method for extraction and detection of flavonoids in galangal) discloses a method using subcritical water as an extraction reagent and provides a High-performance liquid phase detection method provides reliable evidence for flavonoids in ginger

Method used

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  • Study on the extraction process and antioxidant properties of a kind of ginger flavone
  • Study on the extraction process and antioxidant properties of a kind of ginger flavone
  • Study on the extraction process and antioxidant properties of a kind of ginger flavone

Examples

Experimental program
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Effect test

Embodiment 1

[0039] Embodiment 1: the extraction of turmeric

[0040] (1) Take sliced ​​ginger and crush it, weigh 10.0 g of ginger powder, and measure the water content to be 13.7%;

[0041] (2) Use 300 ml of 95% ethanol as the solvent, soak the ginger powder, and let it stand for 2 h;

[0042] (3) Heat the mixture after standing to reflux at 70°C for 2 h; after cooling, filter to obtain the extract;

[0043] (4) Concentrate the extract obtained above to about 30 ml by rotary evaporation, transfer to a 50 ml volumetric flask, dilute to the mark with 75% ethanol, and shake well as the sample solution;

[0044] (5) Take 1.0 ml of the sample solution, transfer it to a 10 ml volumetric flask, dilute to the mark with 75% ethanol, and shake well.

[0045] (6) Take 2.0 ml of the solution, put it in a 10 ml volumetric flask, operate according to the standard curve drawing method, and measure the absorbance, as shown in the attached figure 1 As shown, the flavonoid concentration C is calculate...

Embodiment 2

[0047] Embodiment 2: the mensuration of total reducing ability of turmeric

[0048] (1) Add 2.5 ml of phosphate buffer solution with pH=6.6, 1.0 ml of different concentrations of curcumin extract and 1% potassium ferricyanide solution in sequence in a 10 ml test tube, shake well, and heat in a water bath at 50°C 20 min, rapid cooling;

[0049] (2) Add 2.5 ml of 10% trichloroacetic acid solution, shake well, and centrifuge at 3000 rpm for 10 min;

[0050] (3) Remove 5 ml of the centrifuged supernatant, 4 ml of distilled water and 1.0 ml of 1% ferric chloride solution, mix well and let stand for 10 min;

[0051] (4) Take the above solution to measure the absorbance value at wavelength λ=700 nm, adjust to zero with distilled water, the greater the absorbance, the stronger the reducing ability, and 2,6-di-tert-butyl-p-cresol is used as a control;

[0052] (5) As attached Figure 8 As shown, curcumin has good reducing ability, and its total reducing ability is stronger than that...

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Abstract

The invention discloses an extraction process and anti-oxidation application of ginger flavonoids, which comprises the following steps: take cut ginger slices and pulverize them, sieve and weigh ginger powder, use ethanol as a solvent to heat and reflux for extraction for a period of time, and obtain a filtrate after filtering , dilute the filtrate to measure the absorbance, adopt sodium nitrite-aluminum nitrate-sodium hydroxide chromogenic method to draw the rutin standard curve figure, obtain the concentration of the standard equation to calculate the turmeric flavonoids, then substitute in the formula to calculate the content of the total flavonoids in the ginger, The antioxidant research was carried out again, and it was found that the antioxidant capacity of total flavonoids in ginger was stronger than that of 2,6-di-tert-butyl-p-cresol. The method adopted in the invention is relatively simple, the solvent can be recycled and reused, the cost is low, the extraction rate of turmeric is relatively high, the anti-oxidation effect is good, the process is simple and applicable to large-scale production.

Description

technical field [0001] The invention relates to the technical field of extracting effective components of ginger, in particular to a process for extracting flavonoids in ginger and research on its antioxidant properties. Background technique [0002] Ginger, also known as ginger root and Bailayun, is palm-shaped and is the rhizome of the perennial herb of Zingiberaceae and Zingiberaceae. Ginger is native to the tropical regions of Southeast Asia. It likes a warm and humid climate, and has weak cold and drought resistance. Ginger is a kind of rhizome spice widely grown in the world, and it is also a commonly used medicinal material in traditional Chinese medicine in China and even in the whole of Asia. Ginger skin is pungent and cool, which can cure skin edema. Ginger juice is pungent and warm, and has strong power to dispel stomach cold. It can be used to treat exogenous wind and cold, headache, phlegm, cough, stomach cold and vomiting. Li Shizhen of the Ming Dynasty comme...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/9068A61P39/06G01N21/31A61K125/00
CPCA61K36/9068A61K2236/333A61P39/06G01N21/31
Inventor 宋吉明方晶董庄庄
Owner ANHUI UNIVERSITY
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