Umbilical cord mesenchymal stem cell preserving fluid

Abstract

The invention discloses an umbilical cord mesenchymal stem cell preserving fluid. The umbilical cord mesenchymal stem cell preserving fluid comprising the following components at different concentrations: low-sugar DMEM culture medium of 5 to 10% (V / V), human serum albumin of 15 to 25g / L, sodium chloride of 8 to 10g / L, low-molecular heparin sodium of 2000 to 3000AXaIU / mL, glucose of 20 to 50g / L, vitamin C of 100 to 150mg / L, and salidroside of 10 to 30mu M. The umbilical cord mesenchymal stem cell preserving fluid can effectively preserve the umbilical cord mesenchymal stem cells under the condition of 4 DEG C and keep stable cell phenotypes. All the components of the umbilical cord mesenchymal stem cell preserving fluid are safe and non-toxic to a human body, subsequent use of the umbilical cord mesenchymal stem cells is facilitated, and the umbilical cord mesenchymal stem cell preserving fluid is very suitable for preservation and transportation of the umbilical cord mesenchymal stemcells and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of stem cells, and in particular relates to a preservation solution for umbilical cord mesenchymal stem cells. Background technique [0002] Mesenchymal stem cells (MSCs, Mesenchymal Stem Cells) are derived from mesoderm and ectoderm in the early stages of development. focus on. Mesenchymal stem cells were first found in the bone marrow, and later found in many tissues in the process of human development and development, such as bone marrow, fat, synovium, bone, muscle, lung, liver, pancreas and other tissues, as well as amniotic fluid and umbilical cord blood , placenta, etc. As a widely used stem cell, human umbilical cord mesenchymal stem cells have the advantages of convenient extraction, easy isolation, low immunogenicity, and multilineage differentiation potential, and can be used clinically. [0003] At present, there are still some problems in the application of human umbilical cord mesenchymal st...

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Problems solved by technology

[0003] At present, there are still some problems in the application of human umbilical cord mesenchymal stem cells in scientific research, industry and clinical application, such as inconvenient transportation, inconvenient temporary storage, etc.

Mesenchymal stem cells die and deteriorate quickly at room temperature after leaving the culture conditions. In the non-cultured state, they are generally frozen and stored in liquid nitrogen tanks for long-term storage. The cost of long-distance transportation in small quantities is very high, which greatly limits the clinical application of mesenchymal stem cells. Applied mass production and long-distance supply

In addition, in clinical applications, human umbilical cord mesenchymal stem cell preparations need to complete quality tests such as sterility and activity before they can be injected into the human body. These tests take a long time to obtain reliable results

However, the commonly used low-temperature cryopreservation has great damage to cells and cannot be directly injected into the human body after thawing. Therefore, during the quality inspection of stem cell preparations, a suitable preservation solution is the basis for clinical application.

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