Group of molecular markers capable of being used for early warning of whitening of pavona descussata and application of molecular markers

A molecular marker and early warning technology, applied in the fields of application, plant gene improvement, recombinant DNA technology, etc., can solve the problem of poor understanding of the molecular mechanism of coral bleaching

Inactive Publication Date: 2019-12-27
香港浸会大学深圳研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Coral bleaching is considered an important cause of threats to coral reef ecology

Method used

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  • Group of molecular markers capable of being used for early warning of whitening of pavona descussata and application of molecular markers
  • Group of molecular markers capable of being used for early warning of whitening of pavona descussata and application of molecular markers
  • Group of molecular markers capable of being used for early warning of whitening of pavona descussata and application of molecular markers

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Experimental program
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Effect test

Embodiment 1

[0027] The field monitoring of embodiment 1 coral thermal bleaching and the collection of coral samples

[0028] We chose the common stony coral, Pavona decussata, as a sample to examine coral bleaching and recovery in the wild. Coral bleaching in Hong Kong generally occurs in summer. Since June 2017, we have been regularly monitoring coral bleaching in Hong Kong. At the beginning of July 2017, control and bleached samples of cross peony coral were collected in Kiu Tsui Chau, Hong Kong by scuba diving. At the same time, we collected non-bleached (normal) samples on the bleached population as a comparison, and marked the sampled corals. The recovered samples after bleaching were collected at the end of September of the same year. The bleached samples, normal and recovered samples were all from the same coral colony, and the control samples were from another coral colony. The morphological pictures of healthy and bleached cross peony corals are as follows figure 1 shown.

...

Embodiment 2

[0030] Example 2 Extraction of Coral RNA, Assembly and Annotation of Transcriptome

[0031] Total RNA was extracted using TRIzol reagent according to the reagent instructions, and then treated with RNase-free DNase I. The quality and concentration of RNA samples were determined using 1% agarose gel electrophoresis and a NanoDrop 2000c spectrophotometer. RNA samples for each species consisted of triplicates. RNA sequencing was performed at the Beijing Genomics Institute (BGI) Shenzhen headquarters using the Illumina X-TEN platform. Before building the library, use Bioanalyzer 2100 to further analyze the concentration of RNA samples.

[0032] The library was constructed using the Oligo dT enrichment method and completed with NEBNext Illumina's Ultra RNA library preparation kit. The sequencing length is 151bp. Sequences with high (>10%) N content comparisons or high reads with low quality (Phred value Q≤20, content >40%) were removed.

[0033]Transcriptome assembly was done ...

Embodiment 3

[0037] Differentially expressed genes in corals in embodiment 3

[0038] Coral differentially expressed genes (DEGs) were obtained by pairwise comparison. The number of DEGs in the coral bleaching group, normal group and recovery group compared with the control group is shown in Figure 4 As shown, for corals, there were 2127 DEGs in the bleached group, 924 DEGs in the normal group, and 586 DEGs in the recovery group, indicating that the gene expression of corals in the bleached group differed most from other groups.

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Abstract

The invention discloses a group of molecular markers capable of being used for early warning of whitening of pavona descussata and application of the molecular markers. The molecular markers include an E3 ubiquitin protein ligase TRIM71 gene, a homeobox protein Hox-B7-B gene and a bone organic matrix protein 5 gene. According to the group of molecular markers and the application of the molecular markers, a high-flux transcriptome sequencing technology is used for sequencing a transcriptome of the pavona descussata, and processing data as bioinformatics, transcriptome expression changes of coral in different whitening and recovery stages are compared to understand different molecular response mechanisms of different coral under the same environmental pressure in the field, the group of themolecular markers which can predict the whitening of the coral is found out, scientific data are provided for the final development of a simple and practical early warning kit of the whitening of thecoral, and the group of molecular markers have wide application prospects in the early warning of the whitening of the pavona descussata and the preparation of the early warning kit of the whitening of the pavona descussata.

Description

technical field [0001] The invention belongs to the field of biotechnology. More specifically, it relates to a set of molecular markers and their applications that can be used for early warning of cross peony coral bleaching. Background technique [0002] Coral reefs are formed by the secretion of calcium carbonate skeletons by numerous individual coral polyps. The coral polyps form a mutually beneficial symbiotic relationship with the zooxanthellae in their bodies. The zooxanthellae use the carbon dioxide and nutrients produced by the metabolism of the coral polyps to perform photosynthesis, and part of the products of photosynthesis become the survival of corals. energy. Coral reefs are one of the ecosystems with the richest biodiversity. They have very important ecological functions such as protecting the coastline and providing places for habitat, reproduction and growth of marine organisms. Coral bleaching refers to the destruction of the symbiotic relationship betwee...

Claims

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Application Information

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IPC IPC(8): C12Q1/6888C12N15/52C12N15/12
CPCC07K14/43595C12N9/93C12Q1/6888C12Q2600/158C12Y603/02019
Inventor 张燕杰邱建文
Owner 香港浸会大学深圳研究院
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