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A kind of screening domestication method of natural rubber deodorizing flora

A technology of natural rubber and flora, applied in the direction of using microorganisms, etc., can solve the problems of undetectable, lack of pertinence, low smell threshold, etc., and achieve a simple domestication method, strong deodorization effect, and high deodorization efficiency Effect

Active Publication Date: 2021-12-03
RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of the current gas analysis technology level, the smell threshold of many gases is far lower than the detection line of the instrument, and cannot be detected. As a result, the detected substances are not necessarily the main substances that produce odor, and there are many Odor is composed of a mixture of several gases, so the conventional domestication method of strains, that is, the method of finding out the main substances of the odor through instrumental analysis and then preparing the corresponding medium for targeted screening and domestication is unrealistic
[0005] Therefore, it is difficult to have a biological deodorizing bacteria group specially used for natural rubber processing odor treatment. The current research on rubber processing biological deodorizing bacteria liquid mostly comes from industries such as breeding and garbage disposal. Because of the lack of pertinence, the deodorizing effect is not good. good

Method used

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  • A kind of screening domestication method of natural rubber deodorizing flora
  • A kind of screening domestication method of natural rubber deodorizing flora
  • A kind of screening domestication method of natural rubber deodorizing flora

Examples

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Comparison scheme
Effect test

Embodiment 1

[0071] A method for screening and domesticating natural rubber deodorizing flora, comprising the following steps:

[0072] S1: Bacterial source solution preparation: put 200mL of bacterial source solution in an acclimation bottle;

[0073] S2: Bacterial enrichment culture: add 500mL EM bacteria solution and 3% sugarcane molasses to the acclimatization bottle, and culture at 25°C for 2 days;

[0074] S3: 20Kg of natural rubber wet gel fragments are placed on the grid of the domestication device, and gas is introduced (the air flow rate is 2m 3 / h), make the gas enter the domestication bottle after passing through the natural rubber wet gel fragments, and finish the domestication after 123 days to obtain the deodorizing flora.

[0075] The bacteria source liquid described in step S1 is the effusion of the gel accumulation pool; the accumulation time of the effusion of the gel accumulation pool is 30 days.

[0076] The natural rubber wet gel pieces described in step S3 are repl...

Embodiment 2

[0083] A method for screening and domesticating natural rubber deodorizing flora, comprising the following steps:

[0084] S1: Bacterial source solution preparation: put 50mL of bacterial source solution in an acclimation bottle;

[0085] S2: Bacterial enrichment culture: add 500mL EM bacteria solution and 1% sugarcane molasses to the acclimatization bottle, and culture at 25°C for 1 day;

[0086] S3: 20Kg natural rubber wet gel pieces are placed on the grid of the acclimation device, and gas is introduced (the air flow rate is 1m 3 / h), make the gas enter the domestication bottle after passing through the natural rubber wet gel fragments, and finish the domestication after 123 days to obtain the deodorizing flora.

[0087] The bacteria source liquid described in step S1 is the effusion of the gel accumulation pool; the accumulation time of the effusion of the gel accumulation pool is 30 days.

[0088] The natural rubber wet gel pieces described in step S3 are replaced once ...

Embodiment 3

[0095] A method for screening and domesticating natural rubber deodorizing flora, comprising the following steps:

[0096] S1: Preparation of bacterial source solution: put 550mL bacterial source solution in an acclimation bottle;

[0097] S2: Bacterial enrichment culture: add 500mL EM bacteria solution and 5% sugarcane molasses to the acclimatization bottle, and culture at 25°C for 5 days;

[0098] S3: 20Kg of natural rubber wet gel fragments are placed on the grid of the domestication device, and gas is introduced (the air flow rate is 3m 3 / h), make the gas enter the domestication bottle after passing through the natural rubber wet gel fragments, and finish the domestication after 123 days to obtain the deodorizing flora.

[0099] The bacteria source liquid described in step S1 is the effusion of the gel accumulation pool; the accumulation time of the effusion of the gel accumulation pool is 30 days.

[0100] The natural rubber wet gel pieces described in step S3 are repl...

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Abstract

The invention belongs to the technical field of microbial strain screening, and in particular relates to a method for screening and domestication of natural rubber deodorant flora. A method for screening and acclimation of natural rubber deodorizing bacteria group, comprising the following steps: placing natural rubber wet gel fragments on a partition net, feeding gas, and allowing the gas to pass through the natural rubber wet gel fragments and then enter an acclimation bottle, After 110-150 days of domestication, the deodorant flora is obtained.

Description

technical field [0001] The invention belongs to the technical field of microbial strain screening, and in particular relates to a method for screening and domesticating natural rubber deodorizing bacteria. Background technique [0002] Natural rubber (NR) is a natural polymer compound with cis-1,4-polyisoprene as the main component, and 91% to 94% of its components are rubber hydrocarbons (cis-1,4-polyisoprene Diene), and the rest are non-rubber substances such as protein, fatty acid, ash, sugar, etc. Natural rubber is the most widely used general-purpose rubber. [0003] During the processing of natural rubber, due to the rich nutrients of latex raw materials, it is easy to be utilized and degraded by microorganisms in the environment to release foul odor, which is an urgent problem to be solved in current production. [0004] Comprehensively comparing the current deodorization methods, biological deodorization has become the first choice for natural rubber processing deo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/36
CPCC12N1/36
Inventor 丁丽桂红星李建伟宋亚忠赵立广王岳坤黄红海张小妹
Owner RUBBER RES INST CHINESE ACADEMY OF TROPICAL AGRI SCI
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