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Lactobacillus casei capable of relieving rheumatoid arthritis and application of lactobacillus casei

A technology of Lactobacillus casei and rheumatoid, applied in the field of microorganisms, can solve the problems of high cost, induction of other diseases, excessive suppression of the immune system, etc., and achieve low-cost effects

Active Publication Date: 2019-12-20
无锡食生臻选生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, in the treatment of rheumatoid arthritis (Rheumatoid Arthritis, RA), the clinical use is limited to the use of some biological agents targeting inflammatory factors such as IL-6 or TNFα, and these biological agents are effective in short-term clinical application. However, it can only temporarily relieve and improve the condition, and it is difficult to fundamentally control the development of the condition. It is recurrent and expensive. In addition, biological agents are a cascade reaction that forcibly prevents immune inflammation in the body. Long-term use will cause patients The immune system is oversuppressed, and there is a risk of inducing other diseases

Method used

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  • Lactobacillus casei capable of relieving rheumatoid arthritis and application of lactobacillus casei
  • Lactobacillus casei capable of relieving rheumatoid arthritis and application of lactobacillus casei
  • Lactobacillus casei capable of relieving rheumatoid arthritis and application of lactobacillus casei

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1: Screening and strain identification of Lactobacillus casei

[0064] 1. Screening

[0065] Healthy human feces samples from Wuxi were stored in 30% glycerol in a -80°C refrigerator. After taking out the sample and thawing it at low temperature, mix the sample evenly, draw 0.5mL sample and add it to 4.5mL, carry out gradient dilution with 0.9% normal saline containing 0.05% cysteine, and select the appropriate gradient dilution solution to coat the sample with 0.05% cysteine On the MRS solid medium of cystine, culture at 37°C for 48 hours, pick typical colonies to streak and purify on the MRS solid medium, pick a single colony and transfer to MRS liquid medium (containing 0.05% cysteine) Cultured in , and the cultured cells were preserved in 30% glycerol to obtain strains CCFM1074, L1 and L2.

[0066] 2. Identification

[0067] The genomes of CCFM1074, L1, and L2 were extracted, and the 16S rDNA of CCFM1074, L1, and L2 were amplified and sequenced (by Hua...

Embodiment 2

[0068] Embodiment 2: the cultivation of Lactobacillus casei

[0069] Lactobacillus casei (Lactobacillus casei) CCFM1074 was inoculated into MRS solid medium (containing 0.05% cysteine) and cultured at 37°C for 48 hours, and its colonies were observed, and it was found that the colonies were milky white, with smooth surfaces and round bulges.

[0070] Lactobacillus casei (Lactobacillus casei) CCFM1074 was inserted into MRS liquid medium (containing 0.05% cysteine) and after anaerobic culture at 37 °C for 24 h, it was transferred into fresh MRS liquid medium (containing 0.05% cysteine). ), cultured under the same conditions for 24 hours, centrifuged at 6000g for 15min, washed with 0.9% normal saline and centrifuged again at 6000g for 10min to obtain the bacteria, resuspended with 30% sucrose solution, and frozen at -80°C until use.

Embodiment 3

[0071] Example 3: Tolerance of different Lactobacillus casei to simulated gastrointestinal fluid

[0072] 1. Tolerance of different Lactobacillus casei to simulated gastric juice

[0073] Lactobacillus casei (Lactobacillus casei) CCFM1074, Lactobacillus casei (Lactobacillus casei) L1, and Lactobacillus casei (Lactobacillus casei) L2 obtained in Example 1 were respectively inserted into MRS liquid medium and cultivated at 37°C for 18 hours, and then the cells were collected by centrifugation , the collected cells were washed with normal saline, and after the washing, the cells were centrifuged again to collect the cells, and the collected cells were resuspended in the normal saline containing 3g / L pepsin at pH 3 (pH adjusted by HCl), and kept Lactobacillus casei (Lactobacillus casei) CCFM1074, Lactobacillus casei (Lactobacillus casei) L1, and Lactobacillus casei (Lactobacillus casei) L2 have the same initial concentration in normal saline, take 0.1mL of the bacterial liquid and c...

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Abstract

The invention discloses lactobacillus casei capable of relieving rheumatoid arthritis and application of the lactobacillus casei, and belongs to the technical field of microorganisms. A lactobacilluscasei CCFM1074 strain is screened out by the invention, and the lactobacillus casei CCFM1074 has the effect of relieving rheumatoid arthritis. The effect is specifically reflected in the following aspects: a weight loss of a rat suffering from rheumatoid arthritis is remarkably relieved; content of pro-inflammatory cytokines TNF alpha and IL-1 beta in serum of the rat with rheumatoid arthritis isremarkably reduced; content of short-chain fatty acid in excrement of the rat suffering from rheumatoid arthritis is remarkably adjusted; ratios of Th17 cells and Treg cells in mesenteric lymph nodesof the rat with rheumatoid arthritis are remarkably reduced; intestinal flora of the rat with rheumatoid arthritis is improved at a genus level; and the lactobacillus casei CCFM1074 obviously inhibitsthe growth of pathogenic bacteria Prevotella copri in vitro.

Description

technical field [0001] The invention relates to a Lactobacillus casei strain capable of relieving rheumatoid arthritis and its application, belonging to the technical field of microorganisms. Background technique [0002] Rheumatoid arthritis (RA) is an autoimmune disease with symmetric polyarthritis as the main clinical manifestation. The disease is characterized by chronic inflammation of the synovial membrane and progressive destruction of the joints. The clinical manifestations are varied and the prognosis is often poor. In the late stage, it can cause joint ankylosis, deformity, and severe functional impairment. Visceral and multi-system damage seriously endanger the health and quality of life of patients. [0003] Rheumatoid arthritis (Rheumatoid Arthritis, RA) occurs all over the world, and northern my country is the high incidence area of ​​this disease. Therefore, the prevention and treatment of rheumatoid arthritis (Rheumatoid Arthritis, RA) has important theoret...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/747A61P29/00A61P19/02A61P31/04A23L33/135C12R1/245
CPCA61K35/747A61P29/00A61P19/02A61P31/04A23L33/135A23V2002/00C12R2001/245C12N1/205A23V2400/125A23V2200/30
Inventor 陈卫杨波樊哲新赵建新张灏
Owner 无锡食生臻选生物科技有限公司
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