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Raffinose extraction method

A raffinose and extraction technology, applied in chemical instruments and methods, oligosaccharides, sugar derivatives, etc., can solve the problem of inability to recover or utilize dephenolized raffinose protein, low raffinose solubility, and low raffinose yield and other problems, to achieve the effect of low cost, low equipment requirements and high purity

Active Publication Date: 2019-12-20
CHENGUANG BIOTECH GRP CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to ensure the effect of dephenolization, it is necessary to use a high concentration of organic solvents, which not only causes the solubility of raffinose in it to be very low, generally not more than 2%, which makes the yield of raffinose extracted and recovered from it low, but also causes cottonseed meal The denaturation of the protein in the cottonseed meal makes it impossible to recycle or utilize the dephenolized cottonseed protein in the cottonseed meal, resulting in a waste of protein resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044]Using 60% methanol with a pH of 7.5 to extract defatted cotton meal 7 times in countercurrent circulation to obtain a dephenolized solution, in which the raffinose content was 4.25%. Take 5L of the dephenolized solution and pass it through a nanofiltration membrane with a molecular weight cut-off of 1000Da to obtain the permeate. Use a 200L column to fill the solid phase extraction column with 150ml of neutral alumina filler, the upper column liquid is 2BV, and the flow rate is 1.0BV / h. After passing through the column, rinse with 2BV of 95% methanol, then elute with 1.5BV of deionized water to obtain an eluent, add 0.2% activated carbon to the eluent, adjust the pH value to 5-6, and heat to 50°C Stir for 30 min, and obtain a decolorized solution after filtration. Add 45% ethanol to the decolorizing solution, lower the temperature to 10°C to crystallize for 14 hours, filter to obtain raffinose crystals, wash the crystals with a small amount of 95% ethanol and dry at 80°C...

Embodiment 2

[0046] 50% ethanol solution with pH 8.0 was used to extract defatted cottonseed meal 7 times in a counter-current cycle to obtain a dephenolized liquid with a raffinose content of 4.92%. Take 5L of the dephenolized solution and pass it through a nanofiltration membrane with a molecular weight cut off of 700Da to obtain the permeate. Use a 200L column to fill the solid phase extraction column with 150ml of octadecyl bonded silica gel. The upper column liquid is 3.0BV, and the flow rate is 1.5BV / h. After passing through the column, use 2BV of 95% ethanol to rinse, and then use 1.5BV of deionized water to elute to obtain an eluent, which is decolorized with D001 resin to obtain a decolorized solution. Add 50% ethanol to the decolorizing solution, lower the temperature to 10°C to crystallize for 12 hours, filter to obtain raffinose crystals, wash the crystals with a small amount of 95% ethanol and dry at 80°C to obtain raffinose products, which are analyzed by HPLC, pentahydrate c...

Embodiment 3

[0048] 50% acetone solution with a pH of 9.0 was used to circulate and countercurrent extract the degreased cottonseed meal for 7 times to obtain a dephenolized liquid with a raffinose content of 4.69%. Take 5L of the dephenolized solution and pass it through a nanofiltration membrane with a molecular weight cut off of 700Da to obtain the permeate. Use a 200L column to fill 150ml of polystyrene benzene packing with the permeate liquid on the solid phase extraction column, the upper column liquid is 1.5BV, and the flow rate is 0.5BV / h. After passing through the column, use 3BV of 90% acetone to rinse, and then use 2BV of deionized water to elute to obtain an eluent, which is decolorized with D301 resin to obtain a decolorized solution. Add 60% ethanol to the decolorizing solution, lower the temperature to 4°C to crystallize for 10 hours, filter to obtain raffinose crystals, wash the crystals with a small amount of 95% ethanol and dry at 80°C to obtain raffinose products, which ...

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PUM

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Abstract

The invention discloses a raffinose extraction method. Degreased cotton meal is used as a raw material, an organic solvent aqueous solution is used for extraction and dephenolizaiton, dephenolized cottonseed protein and dephenolization liquid are obtained, and raffinose is extracted from the dephenolization liquid, wherein the organic solvent aqueous solution is a low-concentration weak-alkaline organic solvent aqueous solution. The method has the advantages that operation is simple, raw materials are easy to obtain, and the method is lower in cost, small in pollution, low in equipment requirement and suitable for industrial mass production.

Description

technical field [0001] The invention relates to a method for extracting raffinose, in particular to a process for extracting raffinose from cottonseed meal dephenolization liquid. Background technique [0002] Dephenolized cottonseed protein is a protein product obtained from cottonseed after peeling, shelling, low-temperature leaching to extract oil, leaching with organic solvents to remove gossypol, drying and crushing, and its protein content can reach more than 50%, which is a high-quality protein resource. Cottonseed meal is a cake obtained after cottonseed is pressed, and then most of the residual oil inside is separated through a leaching process to obtain a reddish or yellow granular product. In the prior art, defatted cottonseed meal is usually used as a raw material, and dephenolized with an aqueous solution of an organic solvent to obtain dephenolized cottonseed protein. When organic solvents are used to remove gossypol, part of the raffinose will be extracted, w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H1/08C07H3/06
CPCC07H1/08C07H3/06
Inventor 曹通蔡亚园田洪
Owner CHENGUANG BIOTECH GRP CO LTD
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