Construction method of obstructive sleep apnea and aortic dissection mouse model

Abstract

The invention relates to animal model construction, and particularly discloses a construction method of an obstructive sleep apnea and aortic dissection mouse model. According to the method, a pump isadopted for being implanted subcutaneously to continuously pour angiotensin II into a mouse for constructing the mouse animal model; according to the construction method of the obstructive sleep apnea and aortic dissection mouse model, in combination with an AD mouse model simulating an IHR curve of an adult suffering from the severe OSAS and utilizing an Ang II induction, after the new mouse model is evaluated, people can prove that compared with a control group, an IH group has a higher AD formation rate, a larger maximum aortic diameter and a lower cumulative survival rate. The phenotypedata is consistent with the human discovery, therefore people construct the reliable and easy-to-use mouse model for studying the function of the OSAS in AD.

Description

technical field [0001] The invention relates to the construction of animal models, in particular to a method for establishing a mouse model of obstructive sleep apnea with aortic dissection. Background technique [0002] Aortic dissection refers to the sudden tearing of the aortic intima caused by various reasons, and the circulating blood enters the middle layer of the vessel wall and causes its stratification. Driven by the pressure of the blood flow, it gradually expands in the aortic media to form a dissection . Acute onset, sudden severe chest pain, shock and involvement of corresponding aortic branch vessels lead to acute ischemia of organs are the characteristics of the disease. In my country, with the aging of the population and the change of diet structure, the incidence of AD is rising rapidly. Epidemiology found that many cardiovascular risk factors such as smoking, male, age, hypertension and atherosclerosis are closely related to the pathogenesis of AD. Despi...

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Problems solved by technology

[0005] At present, there are many studies on aortic dissection models, including surgical production, drug stimulation and other methods, but there is still a lack of classic, unified and effective animal models of aortic dissection, especially those combined with OSA. Therefore, the impact of intermittent hypoxia The pathophysiological research on the formation of aortic dissection urgently needs experimental animal models that can simulate the pathological anatomy and pathophysiology of human aortic dissection with OSA

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