A chemiluminescent kit for quantitative detection of canine distemper virus-canine parvovirus antibody by one-step method

A chemiluminescence reagent and canine distemper virus technology, which is applied in chemiluminescence/bioluminescence, biological testing, and analysis through chemical reactions of materials, etc., can solve the problem of high technical requirements of ELISA detection and multiple rapid tests for unsuitable individuals , poor repeatability of accuracy and other issues, to achieve good application prospects, realize automatic operation, and improve the effect of accuracy

Active Publication Date: 2022-04-01
QINGDAO HARRENS INSPECTION & TESTING INC
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Problems solved by technology

[0003] At present, ELISA and immunochromatography are mostly used for routine antibody detection. ELISA has high technical requirements and requires professional and technical personnel. It is suitable for batch detection and not suitable for individual rapid multi-test. Advantages, but there are also disadvantages such as low sensitivity, poor accuracy and repeatability, and difficulty in quantification

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  • A chemiluminescent kit for quantitative detection of canine distemper virus-canine parvovirus antibody by one-step method

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Embodiment 1

[0027] Refer to attached figure 1 , a kind of chemiluminescent kit for one-step quantitative detection of canine distemper virus-canine parvovirus antibody of the present invention, strip tube design, mainly includes anti-canine IgG immune microspheres (reaction tube 1), sample treatment solution (sample Processing tube 2), fluorescent reaction solution (reagent tube 3), washing solution (reagent tube 4) and spare tubes 5-8.

[0028] The preparation method of the anti-canine IgG immune microspheres is as follows: absorb 1 mL of carboxyl magnetic beads with a diameter of 100-300 nm into a centrifuge tube, place the centrifuge tube in a magnetic stand to separate the magnetic beads from the buffer, and use 0.1M pH5.0 Wash the magnetic beads 4-8 times with 2-(N-morpholine)ethanesulfonic acid, add 500uL of 0.1M pH 5.0 2-(N-morpholine)ethanesulfonic acid solution to the above-mentioned washed magnetic beads, and resuspend. Add 10mg / mL of 1-(3-dimethylaminopropyl)-3-ethylcarbodiimi...

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Abstract

The invention discloses a chemiluminescence reagent kit for quantitatively detecting canine distemper virus-canine parvovirus antibody by one-step method, which belongs to the technical field of biological detection. A chemiluminescence kit for one-step quantitative detection of canine distemper virus-canine parvovirus antibody of the present invention is composed of anti-canine IgG immune magnetic beads, fluorescent reaction liquid, sample treatment liquid, and washing liquid; wherein, the fluorescent The reaction solution is a solution of different fluorescently labeled canine parvovirus antigens and canine distemper virus antigens. The detection kit of the invention can quickly and accurately differentiate and diagnose the levels of canine distemper virus antibody and canine parvovirus antibody at the same time, has the characteristics of strong specificity, high sensitivity, good repeatability, easy operation and the like, and has good application prospects.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a chemiluminescence reagent kit for one-step quantitative detection of canine distemper virus-canine parvovirus antibody. Background technique [0002] In recent years, with the increase in the number of military dogs, police dogs, experimental dogs and pet dogs in my country, and the increase in exchanges between different places, the morbidity and mortality rates of canine distemper and canine parvovirus disease in dogs in my country are on the rise. Both canine distemper and canine parvovirus are acute, high-incidence infectious diseases, and both show similar symptoms such as high fever, vomiting, and diarrhea. Canine distemper (CD) is an acute and highly contagious viral infectious disease of dogs caused by canine distemper virus (CDV) infection worldwide. Hosts include most carnivores. CD mainly occurs in puppies, early manifested as early biphasi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/68G01N33/569G01N33/553G01N33/543G01N33/533G01N33/58G01N21/76
CPCG01N33/6854G01N33/56983G01N33/54326G01N33/553G01N33/533G01N33/582G01N21/76G01N2333/13
Inventor 刘刚梁德勇单虎尹青青杨珍尹召花
Owner QINGDAO HARRENS INSPECTION & TESTING INC
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