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Fluorescence labeling composite amplification kit for human Y chromosome STR loci and InDel sites and application of thereof

A Y chromosome and fluorescent labeling technology, applied in the field of forensic genetics, can solve the problems of small number and difficult sample family distinction, and achieve the effect of accurate typing results, high sensitivity and strong specificity

Active Publication Date: 2019-12-13
湖南省公安科学技术研究所 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, some kits on the market already contain both Y-STR and Y-InDel, such as: AGCU Y37+5, Y Filer Platinum, DNA Typer 36Y, Goldeneye DNA 38Y, etc., but these kits generally contain Y-InDel sites in 3-5, the number is small, and it is difficult to effectively distinguish the sample families. The present invention adds 8 Y-InDel sites on the basis of using 37 Y-STRs, which greatly improves the ability of the kit to distinguish families , and 37 Y-STRs have a strong ability to identify different male individuals

Method used

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  • Fluorescence labeling composite amplification kit for human Y chromosome STR loci and InDel sites and application of thereof
  • Fluorescence labeling composite amplification kit for human Y chromosome STR loci and InDel sites and application of thereof
  • Fluorescence labeling composite amplification kit for human Y chromosome STR loci and InDel sites and application of thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] 1. Y-STR locus screening

[0040] 通过对DYS19、DYS385a / b、DYS389I、DYS389II、DYS390、DYS391、DYS392、DYS393、DYS437、DYS438、DYS439、DYS448、DYS456、DYS458、DYS635、Y_GATA_H4、DYS481、DYS533、DYS576、DYS643、DYS460、DYS549、DYF387S1a、 DYF387S1b、DYS449、DYS518、DYS627、DYS570、DYS527a / b、DYS447、DYS444、DYS557、DYS596、DYS446、DYS510、DYS622、DYS443、DYS587、DYS522、Y_GATA_A10、DYS520、DYS552、DYS593、DYS531、DYS459a / b、DYS508、 DYS388, DYS617, DYS645, DYS713, DYS630, DYF404S1, DYS626, DYS526a / b, a total of 59 Y-chromosomal short tandem repeat loci and their allelic genetic characteristics were thoroughly studied, and 37 Y-chromosome short tandem repeats were screened out 序列基因座:DYS392、DYS389I、DYS447、DYS389II、DYS438、DYS527a、DYS527b、DYS522、DYS596、DYS391、DYS456、DYS19、DYS448、DYS520、DYS627、DYS557、DYS437、DYS481、DYS533、DYS390、DYS385a、DYS385b、DYF387S1a、 DYF387S1b, DYS460, DYS393, Y_GATA_H4, DYS439, DYS635, DYS444, DYS643, DYS549, DYS576, DYS458, DYS570, DYS449, DYS518, these 37 Y-chromosomal short tandem repeat loci have hig...

Embodiment 2

[0060] Example 2 Application of Y-InDel in family investigation

[0061] (1) Two blood spot samples from different families: The samples were provided by a public security bureau, both were Chinese citizens and had no ancestry from other countries.

[0062] (2) Use this kit to perform typing detection on the above two samples, and compare the Y-InDel and Y-STR typing of the two samples, and the results are shown in Table 4.

[0063] The specific steps for using the fluorescent labeling multiplex amplification kit are as follows:

[0064] A. Prepare the amplification system, the PCR amplification system is, Reaction Mix 10.0μL, the genomic DNA Xμl content is 0.125-5ng (the optimal template amount range is 0.125-5ng, when the sample DNA content is within this range, high mass amplification map), primer mix 5.0 μL, hot start Taq enzyme (5U / μL) 0.5 μL, sdH 2 O to make up to 25.0 μL.

[0065] B. Amplification thermal cycle, the amplification program of the thermal cycler is: ini...

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Abstract

The invention belongs to the technical field of biological detection, and particularly discloses a fluorescence labeling composite amplification kit for human Y chromosome STR loci and InDel sites, the kit comprises 41 pairs of specific primers, and 37 Y chromosome STR loci and 8 Y chromosome InDel site can be amplified in a single tube. The kit provided by the invention has the characteristics ofstrong specificity, accurate typing, high sensitivity and the like, and 8 InDel sites are added at the same time, so that families of samples can be distinguished to a certain extent. The method canmeet the requirements of case detection, database construction, paternity test and the like.

Description

technical field [0001] The invention belongs to the field of forensic genetics, and relates to a fluorescent label multiple amplification test system, in particular to a fluorescent label multiple amplification kit for human Y chromosome STR loci and InDel sites and an application thereof. Background technique [0002] Short tandem repeat loci (short tandem repeat, STR), as the second-generation genetic marker after restriction fragment length polymorphism, have been widely used in forensic evidence since the 1980s and 1990s. It is one of the most widely used genetic markers for research and identification. Compared with other genetic markers, STR markers have small fragments and are easy to amplify, and are more suitable for trace and degraded samples, and multiple STR loci can be simultaneously amplified, which is fast, efficient, accurate and sensitive It is widely used in individual identification and paternity identification in forensic evidence. The Y chromosome STR ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/156Y02A50/30
Inventor 尹坚英赵杨陈水琴冯锐李琼赵霖付永郑卫国史明皓张雷王皋陈林丽
Owner 湖南省公安科学技术研究所
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