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A nano-diagnosis agent, preparation method and application based on gold nanorods

A gold nanorod and nanotechnology, which is applied in the field of nano-diagnosis and treatment agents based on gold nanorods, can solve the problems of poor photostability and achieve the effects of increasing water solubility, good photoacoustic imaging, and excellent photothermal conversion ability

Active Publication Date: 2022-04-01
SHENZHEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a nano-diagnostic agent, preparation method and application based on gold nanorods in view of the above-mentioned defects of the prior art, aiming at solving the problem of using gold nanorods as a photoacoustic imaging contrast agent in the prior art. It is biologically toxic and has poor photostability

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  • A nano-diagnosis agent, preparation method and application based on gold nanorods
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  • A nano-diagnosis agent, preparation method and application based on gold nanorods

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preparation example Construction

[0046] Such as figure 1 As shown, the preparation method of a gold nanorod-based nano-diagnostic agent described in the embodiment of the present invention comprises the following steps:

[0047] Step S100, adjusting the pH value of the growth solution to the first predetermined pH value range, adding gold seed solution to prepare a gold nanorod solution; wherein, the growth solution includes a gold source, AgNO 3 , reducing agents and surfactants.

[0048] Specifically, gold seed solution was prepared in advance, HAuCl 4 Add water and cetyltrimethylammonium bromide solution to stir, then add ice NaBH 4 Vigorously stir, place at 30-40°C and keep warm for 1-4 hours to obtain the gold seed solution. Of course, other methods can also be used to prepare the gold seed solution, for example, using sodium citrate to reduce HAuCl in boiling water 4 A gold seed solution was obtained.

[0049] Gold source solution (such as HAuCl 4 and / or fluoroauric acid), acid solution (i.e. H 2...

specific Embodiment 1

[0058] 1) HAuCl 4 (17.16 μL, 0.04856M) and 616.16 μL of pure water were added to 2.5 mL of cetyltrimethylammonium bromide (0.1M) solution and stirred for 1 minute, then 200 μL of ice NaBH was added 4 (0.01M) vigorously stirred for 2 minutes, placed at 37 ° C for 2 hours to obtain the gold seed solution. Subsequently to prepare the growth solution, 103 μL HAuCl 4 solution (0.04856M), 200μL H 2 SO 4 (0.5M), 110 μL AgNO 3 (10mM) was sequentially added to 10mL CTAB (0.1M) solution and stirred for 1 minute. Then add 100 μL of ascorbic acid (0.1M) and stir vigorously for two minutes until the solution turns from yellow to clear, then add 24 μL of gold seed solution and continue stirring for 2 minutes, and react at 37°C for 24 hours to prepare a gold rod solution.

[0059] 2) Take 10 mL of the prepared GNR solution and centrifuge once (10000 rpm, 10 minutes) and disperse it into the original volume of water, adjust its pH to about 10 with 10% (v / v) ammonia water, add 500 μL of 1...

specific Embodiment 2

[0063] Different from the specific example one, the HAuCl in the growth solution 4 solution, AgNO 3 , CTAB, and ascorbic acid have different ratios, thereby obtaining nanometer therapeutic agents with aspect ratios of gold nanorods of 2.5, 2.6, 2.7, and 2.8.

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Abstract

The invention discloses a nano-diagnosis agent based on gold nanorods, a preparation method and an application thereof. The nano-diagnosis agent comprises: gold nanorods, a silicon dioxide layer and a metal oxide coated on the gold nanorods in sequence Floor. The silicon dioxide layer in the nano-therapeutic agent greatly increases the water solubility, biocompatibility, photoacoustic imaging stability and photothermal stability of the material, while the metal oxide layer changes the surface plasmon resonance effect of gold nanorods. , thus causing the absorption red shift to reach the second near-infrared region, and further enhancing the stability and photothermal stability of gold nanorod photoacoustic imaging. In addition, nanoparticles exhibit good photoacoustic imaging in the second near-infrared region and have excellent photothermal conversion capabilities, and degrade in the tumor microenvironment to release metal ions, and can also be used for magnetic resonance imaging of tumor stimulus responses.

Description

technical field [0001] The invention relates to the field of nano-diagnosis and treatment agents, in particular to a nano-diagnosis and treatment agent based on gold nanorods, a preparation method and an application. Background technique [0002] Gold nanorods (gold nanorods, GNRs) have received extensive attention and applications in the field of biomedicine due to their unique structural and functional characteristics. The longitudinal plasmon absorption peak of GNR can be adjusted to the near-infrared region, so it can efficiently convert near-infrared light energy into heat energy, and is often used in photoacoustic imaging (PAI) and photothermal therapy (PTT) of tumors. PAI is an emerging non-invasive and non-ionizing imaging. The contrast agent absorbs pulsed laser energy and converts it into heat energy, causing thermoelastic expansion, thereby generating acoustic signals, which are collected by ultrasound probes for imaging. The surface plasmon effect of gold nanoro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/22A61K49/18A61K41/00A61P35/00
CPCA61K49/225A61K49/1884A61K49/0002A61K41/0052A61P35/00
Inventor 黄鹏蒋超贺婷林静
Owner SHENZHEN UNIV
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