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Bacillus arborii, its metabolites and their application

A technology of Bacillus agri

Active Publication Date: 2021-04-30
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The technical problem to be solved in the present invention is to provide a strain of Bacillus arborii that inhibits the growth of rhizopus, its metabolites and its application, in order to solve the control of plant wilt in the prior art (such as in terms of environmental friendliness, control effect, etc.) Unsatisfactory technical issues

Method used

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  • Bacillus arborii, its metabolites and their application
  • Bacillus arborii, its metabolites and their application
  • Bacillus arborii, its metabolites and their application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1: Screening and Identification of Biocontrol Strains

[0028] (1) screening

[0029] In December 2018, Wang Enzhao collected the healthy rice rhizosphere soil in the pot experiment in the greenhouse of the Chinese Academy of Agricultural Sciences, that is, removed about 5 cm of the covering soil on the root surface from the rice, and randomly collected the loose soil combined with the rice root system in an S shape.

[0030] The control group was sterile water, and the test group was soil sample suspension with gradient dilution.

[0031] The specific operation method of the test group is as follows:

[0032] Use the gradient dilution coating method to apply the suspension of the soil sample diluted step by step on the beef extract peptone plate, pick a single colony into the liquid beef extract peptone medium to obtain the bacterial liquid, and take each bacterial liquid and spread it evenly on the beef extract peptone plate. Cultivate on paste peptone mediu...

Embodiment 2

[0046] Embodiment 2: the identification of Bacillus arborii antibacterial metabolites

[0047] Volatile organic compounds are solids or liquids with carbon as the basic element, which can quickly volatilize at 20°C and 0.01 kPa and enter the gas phase. Based on the inventor's long-term accumulated research experience in the early stage, studies have confirmed that such microorganisms can produce a large number of volatile organic compounds with rich types and diverse functions. The main functions of these substances are: as signal substances between communities and within communities; Signaling substances among them; possible carbon release channels; growth-promoting and growth-inhibiting factors. If they function as inhibitors and the phytopathogenic microorganisms are inhibited in the interaction, then the microorganisms releasing volatile organic compounds have the potential to be applied to the biological control of plant diseases.

[0048] (1) Collection of volatile organi...

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Abstract

The invention discloses a bacillus arborii, its metabolites and applications thereof, aiming to solve the technical problem of unsatisfactory control of plant wilt in the prior art. Bacillus arborii Bacillus aryabhattai CGMCC NO.18285 can effectively inhibit Rhizopus microsporus, and can be used to prevent and treat plant diseases caused by Rhizopus microsporus, so as to avoid or reduce the use of pesticides and ensure sustainable agricultural development. It has broad application prospects ; Bacillus arborii CGMCC NO.18285 of the present invention can metabolize and secrete a large amount of volatile substance 5-methyl-2-hexanone, and the present invention first studies and finds that 5-methyl-2-hexanone has a good ability to inhibit microspore roots The role of mold.

Description

technical field [0001] The invention relates to the technical field of biological control, in particular to bacillus arborii, its metabolites and applications thereof. Background technique [0002] Rice is the main food crop in my country, accounting for 60% of the country's food production. Ensuring the safety of rice production is of great significance to my country's agricultural production. Fusarium wilt of rice seedlings caused by rhizopus is a widespread plant disease in Asia, which seriously threatens crop yield. Therefore, solving the disease is of great significance to ensure the safety of my country's grain production. [0003] At present, the control methods for Fusarium wilt are mainly to prevent and control Fusarium wilt by using various methods such as chemical pesticide control, agricultural crop rotation control, and the use of disease-resistant varieties. [0004] Chemical control has the advantages of high efficiency and quick effect, and is a common cont...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/26A01N63/22A01N35/02A01P3/00C12R1/07
CPCA01N35/02C12P7/26C12N1/205C12R2001/07
Inventor 范分良王恩召宋阿琳刘雄舵李艳玲
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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