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A kind of fermentation method that improves fusothermal bacillus cellulase enzyme activity

The technology of a kind of fusothermal bacterium and fermentation method is applied in the fermentation field of improving the enzymatic activity of fusothermal bacterium cellulase, which can solve the problems of high cost, low enzyme activity, and limitation of wide application, and achieve high heat resistance and moisture resistance, Effect of improving cellulase activity and optimizing fermentation process

Active Publication Date: 2021-11-02
JIANGNAN UNIV
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  • Application Information

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Problems solved by technology

The second point is that Trichoderma reesei is the most commonly used strain at present, and its enzyme production ability is equivalent to that of Fusothermal bacteria. However, due to its characteristics and culture conditions, the fermentation time of cellulase is very long and the efficiency is low, resulting in its cost. Too high, the current production of cellulase by liquid fermentation is suitable for large-scale production, but the low enzyme activity of the current fermentation broth leads to high prices of cellulase products produced by liquid methods, which limits its wide application in industry

Method used

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  • A kind of fermentation method that improves fusothermal bacillus cellulase enzyme activity
  • A kind of fermentation method that improves fusothermal bacillus cellulase enzyme activity
  • A kind of fermentation method that improves fusothermal bacillus cellulase enzyme activity

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Embodiment 1

[0023] Preparation of cellulase under different fermentation temperatures in embodiment 1

[0024] Set different fermentation temperatures, respectively 30, 40, 50, 55, and 60° C., and ferment for 72 hours. Cellulase activity was measured after fermentation. The results are shown in Table 1. It was found that the relative enzyme activity of the bacteria could reach more than 90% under the condition of 50-60°C.

[0025] Table 1 Effect of different fermentation temperatures on enzyme activity

[0026]

Embodiment 2

[0027] Preparation of cellulase under the different fermentation time of embodiment 2

[0028] Set different cultivation time, respectively 24h, 48h, 60h, 72h, 84h, 96h, culture at 60°C, and measure cellulase activity after fermentation. The results are shown in Table 2. With the increase of fermentation time, the enzyme activity showed an upward trend. The enzyme activity reached a peak at 60 hours of fermentation, and the enzyme activity began to decline at 96 hours. Therefore, the fermentation is stopped after 60 hours, which can greatly save the fermentation cost.

[0029] Table 2 Effects of different fermentation times on enzyme activity

[0030]

Embodiment 3

[0031] Preparation of cellulase under different initial pHs of embodiment 3

[0032] Control other conditions unchanged, set different initial pH of the medium, respectively 5.5, 6, 6.5, 7, 7.5, 8, ferment at 60°C for 72 hours, and measure cellulase activity after fermentation. The results are shown in Table 3. When the initial pH of the culture medium is 6.5-8, the relative enzyme activity can reach more than 90%.

[0033] Table 3 Effect of initial pH of different medium on enzyme activity

[0034]

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Abstract

The invention discloses a fermentation method for improving the enzyme activity of fusothermal bacillus cellulase, which belongs to the field of fermentation process. The invention optimizes the fermentation process of the fusothermic bacillus, so that the enzyme activity of the cellulase is increased from 10.85U / mL to 16.56U / mL, and the activity of the cellulase is increased by 52% compared with that before the optimization. And according to the growth condition of the bacterium, the carbon source is added by feeding, which greatly improves the cellulase activity and saves the cost, and is suitable for industrial production. The enzyme preparation produced by the invention has extremely high heat resistance and moisture resistance, and after three months of storage, the enzyme activity still remains above 90%, which is suitable for industrial production.

Description

technical field [0001] The invention relates to a fermentation method for improving the enzymatic activity of fusothermal bacillus cellulase, which belongs to the field of fermentation engineering. Background technique [0002] Lignocellulosic raw materials come from a wide range of sources and are renewable resources with abundant reserves. Cellulase is a general term for a group of enzymes that can degrade cellulose into glucose. Lignocellulose can be hydrolyzed by cellulase to produce sugar, which can be directly used in microbial fermentation and can convert cellulose into valuable primary metabolism. The product, cellulase, is often used in food and feed industry, paper industry, medicine, bioenergy and other industries, and has a good application prospect. [0003] Bacteria that produce cellulase include bacteria, fungi, actinomycetes, etc. Among these microorganisms, there is a species called Fusobacterium, which is famous for its high cellulolytic activity, and the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/42C12N9/96C12N1/20C12R1/145
CPCC12N1/20C12N9/2437C12N9/96
Inventor 邓禹毛银陆春波卫亚峰李国辉赵运英周胜虎
Owner JIANGNAN UNIV
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