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A recyclable immunoassay for tumor markers based on core-shell nanomaterials

A technology of core-shell nanomaterials and immunoassay methods, which is applied in the direction of analysis of materials, material excitation analysis, ferrous iron oxides, etc., can solve the problems of high cost and unfavorable practical application, and achieve enhanced efficiency and simple sample cleaning and separation fast effect

Active Publication Date: 2022-06-28
浙江高美基因科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the materials based on surface-enhanced Raman scattering technology are mostly noble metal nanomaterials, which are expensive to prepare, which is not conducive to their practical application in clinical immunoassays.

Method used

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  • A recyclable immunoassay for tumor markers based on core-shell nanomaterials
  • A recyclable immunoassay for tumor markers based on core-shell nanomaterials
  • A recyclable immunoassay for tumor markers based on core-shell nanomaterials

Examples

Experimental program
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Effect test

Embodiment 1

[0030] a Fe 3 O 4 / TiO 2 / Ag triple core-shell nanomaterial and its direct recyclable immunodetection method for prostate specific antigen PSA, comprising the following steps:

[0031] 1. Fe 3 O 4 / TiO 2 Preparation of Ag / Ag triple core-shell nanomaterials

[0032] (1) Dissolve 3.5 grams of ferric chloride hexahydrate, 10.8 grams of sodium acetate and 3 grams of polyethylene glycol in 120 milliliters of ethylene glycol, ultrasonically until completely dissolved, transfer to a hydrothermal reactor, and heat at 200° C. The reaction was carried out for 10 hours, and the precipitate was collected after centrifugation and washing with ethanol to obtain Fe 3 O 4 Nanoparticles;

[0033] (2) Weigh 50 mg of Fe obtained in step (1) 3 O 4 The nanoparticles were dissolved in an aqueous solution of hydrochloric acid with a concentration of 0.1 mmol / ml and ultrasonicated for 15 minutes, magnetically separated and washed three times with deionized water; after magnetic separation a...

Embodiment 2

[0050] With above-mentioned embodiment 1, its difference is: Fe 3 O 4 / TiO 2 In the preparation of Ag core-shell nanomaterials: in step (1), 1.35 g of ferric chloride hexahydrate, 3.6 g of sodium acetate and 1.0 g of polyethylene glycol were dissolved in 40 ml of ethylene glycol, and ultrasonicated until completely dissolved. , transferred to a hydrothermal reactor, reacted at 200°C for 10 hours, centrifuged and washed with ethanol to collect the precipitate to obtain Fe 3 O 4 Nanoparticles; the volume of tetrabutyl titanate added in step (2) is 1 ml.

[0051] Figure 10 For the Raman spectrum, the frequency shift is 1264cm -1 The graph of the characteristic peak intensity changes with the concentration of the antigen to be tested. It can be seen by fitting that when the concentration of the antigen to be tested changes from 100 pg / ml to 0.1 mg / ml, the Raman characteristic peak intensity changes linearly with the concentration. The fitting result shows that this change t...

Embodiment 3

[0053] With above-mentioned embodiment 1, its difference is: Fe 3 O 4 / TiO 2 In the preparation of Ag core-shell nanomaterials: in step (1), 6.75 grams of ferric chloride hexahydrate, 18.0 grams of sodium acetate and 5.0 grams of polyethylene glycol were dissolved in 200 ml of ethylene glycol, and sonicated until completely dissolved. , transferred to a hydrothermal reactor, reacted at 200°C for 10 hours, centrifuged and washed with ethanol to collect the precipitate to obtain Fe 3 O 4 Nanoparticles; the volume of tetrabutyl titanate added in step (2) is 6 ml.

[0054] Figure 11 For the Raman spectrum, the frequency shift is 1264cm -1 The graph of the characteristic peak intensity changes with the concentration of the antigen to be tested. It can be seen by fitting that when the concentration of the antigen to be tested changes from 100 pg / ml to 0.1 mg / ml, the Raman characteristic peak intensity changes linearly with the concentration. The fitting result shows that this...

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Abstract

The invention discloses a preparation method of iron ferric oxide / titanium dioxide / silver core-shell nanomaterial and its direct recyclable immunoassay application. The Raman scattering effect realizes the direct Raman detection of the antigen to be tested. After the Raman detection is completed, the above-mentioned immunocombined nanomaterials are subjected to ultraviolet light to degrade the antigen, and the Fe that has no antigen on the surface is recovered by magnetic force. 3 o 4 / TiO 2 / Ag core-shell nanomaterials, and use it in the next detection of the antigen to be tested, repeat this Raman detection-catalytic degradation-Raman detection process, to achieve direct recyclable immune detection of tumor markers, the advantage is three layers The core-shell structure not only has the functions of magnetic separation and Raman enhancement, but also has the function of catalytically degrading tumor markers. At the same time, the detection limit is low and macromolecular tumor markers can be detected.

Description

technical field [0001] The present invention relates to the field of material engineering and nanotechnology, in particular to a kind of iron tetroxide / titanium dioxide / silver (Fe 3 O 4 / TiO 2 / Ag) preparation method of core-shell nanomaterial and its application in circulating immunodetection. Background technique [0002] In recent years, with the intensification of environmental pollution, the continuous change of the global climate, the occurrence of food crisis and the increase of people's living pressure, cancer frequently occurs, and its high mortality has become a serious threat to human health. Therefore, a series of advanced immune technologies, such as chemiluminescence and enzyme-linked immunoassay, have been developed to detect and treat cancer patients. However, for patients in the early stages of cancer, their blood levels of cancer markers are low, making it difficult to detect and treat early. The surface-enhanced Raman scattering immune technology devel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/532G01N33/543G01N33/537G01N21/65C01G49/08C01G23/053
CPCG01N33/532G01N33/54326G01N33/537G01N21/65C01G49/08C01G23/053C01P2004/04C01P2004/03
Inventor 姜涛周骏姜勇杜远远顾辰杰王福艳
Owner 浙江高美基因科技有限公司
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