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Major QTL site of content of oleic acid of brassica napus seeds, SNP molecular markers and application thereof

A technology of Brassica napus and molecular markers, which is applied in the direction of recombinant DNA technology, microbial measurement/inspection, DNA/RNA fragments, etc., can solve the problems of oil deterioration and unfavorable storage, and achieve high contribution rate, accelerated process, suitable The effect of large-scale promotion and application

Active Publication Date: 2019-12-03
INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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AI Technical Summary

Problems solved by technology

In terms of food quality, rapeseed oil with high oleic acid content has relatively low polyunsaturated fatty acid content, strong thermal stability, and is not easily oxidized during purification and heating, while linolenic acid is easily oxidized under high temperature or strong light conditions. Oxidation leads to oil deterioration, which is not conducive to storage

Method used

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  • Major QTL site of content of oleic acid of brassica napus seeds, SNP molecular markers and application thereof
  • Major QTL site of content of oleic acid of brassica napus seeds, SNP molecular markers and application thereof
  • Major QTL site of content of oleic acid of brassica napus seeds, SNP molecular markers and application thereof

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Embodiment 1

[0031] The mensuration of the phenotype of embodiment 1 Brassica napus oleic acid content

[0032] 1. Determination of Seed Oleic Acid Content Phenotypes in Associated Populations

[0033] (1) A natural population of 324 high-generation lines of Brassica napus from all over the world was formed, and a 3-year and 2-point survey of seed oleic acid content was completed at the Yangluo Experimental Base of Wuhan City and the Experimental Base of Yangzhou Academy of Agricultural Sciences.

[0034] (2) The seedlings were fixed by direct seeding, the row spacing was 33 cm, the plant spacing was 15 cm, 4 rows in each plot, and three replicates were randomly designed. Protective rows were planted around the test material fields.

[0035] (3) Seed oleic acid content: Take 10 mature seeds from each plot to measure the oleic acid content with a near-infrared detector.

[0036] (4) Use the BLUP method (http: / / www.extension.org / pages / 61006) to integrate multi-year multi-point phenotypic dat...

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Abstract

The invention provides a major QTL site of the content of oleic acid of brassica napus seeds. The major QTL site is located between the 8024751st base and the 11299788th base of an A08 chromosome of brassica napus, and preferably has a contribution rate of 71.00 percent for the content of oleic acid of brassica napus seeds. The major QTL site is closely linked with a first SNP molecular marker which is located on the 8024751st base and is C or G, and the mutation causes polymorphism; the major QTL site is closely linked with a second SNP molecular marker which is located on the 11299788th baseand is A or G, and the mutation causes polymorphism; and the major QTL site is closely linked with a peak SNP molecular marker which is located on the 10445600th base and is A or G, and the mutationcauses polymorphism. The invention further provides related SNP molecular markers and application. The major QTL site of the content of oleic acid of brassica napus seeds has a high contribution ratefor the content of oleic acid of brassica napus seeds, has a key effect on regulation of the content of oleic acid of brassica napus seeds, can be used as map-based cloning and molecular marker assisted selection and is suitable for large-scale popularization and application.

Description

technical field [0001] The invention relates to the technical fields of molecular biology and rapeseed breeding, in particular to the technical field of oleic acid content in Brassica napus seeds, in particular to SNP molecular markers closely linked to major QTL sites of oleic acid content in Brassica napus seeds and its application. Background technique [0002] Rapeseed (Brassica napus) is an important oil crop in the Brassicaceae family. It is the main oil crop widely planted in the world and the only winter oil crop in my country. Therefore, the country regards the development of rapeseed production as the focus of ensuring the safe supply of edible oil, which has important practical significance. Brassica napus in my country has become the main cultivated variety due to its characteristics of disease resistance, high yield, wide adaptability, and strong retrograde tolerance. Rapeseed production can be increased mainly through three ways: high rapeseed yield per unit a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12N15/11
CPCC12Q1/6895C12Q2600/156C12Q2600/13
Inventor 刘胜毅张园园唐敏强吴渝刘越英程晓辉
Owner INST OF OIL CROPS RES CHINESE ACAD OF AGRI SCI
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