Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

MUC16 targeted chimeric antigen receptor and MUC16 targeted chimeric antigen receptor T cells and preparation method and application of MUC16 targeted chimeric antigen receptor T cells

A chimeric antigen receptor, CAR-MUC16 technology, applied in the field of medical biology, can solve the problem of low cure rate of ovarian cancer, achieve the effect of efficient and specific killing of tumor cells and promoting expansion

Inactive Publication Date: 2019-12-03
SHENZHEN BINDEBIOTECH CO LTD
View PDF5 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the cure rate of ovarian cancer is generally low

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • MUC16 targeted chimeric antigen receptor and MUC16 targeted chimeric antigen receptor T cells and preparation method and application of MUC16 targeted chimeric antigen receptor T cells

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0079] A method for preparing a chimeric antigen receptor T cell targeting MUC16, comprising the following steps:

[0080] (1) Prepare the gene sequence of the chimeric antigen receptor CAR-MUC16 targeting MUC16

[0081] The genes encoding the signal peptide, the single-chain antibody targeting MUC16, the hinge region of CD8α, the transmembrane region of CD8, the signal region of CD27, and the signal region of CD3ζ were prepared respectively. The genes encoding the signal peptide are shown in SEQ ID NO: 14, and the The coding gene of the single-chain antibody targeting MUC16 is shown in SEQ ID NO: 5, the coding gene of the CD8α hinge region is shown in SEQ ID NO: 7, and the coding gene of the CD8 transmembrane region is shown in SEQ ID NO 9, the coding gene of the CD27 signal region is shown in SEQ ID NO: 10, and the coding gene of the CD3ζ signal region is shown in SEQ ID NO: 12.

[0082] The genes encoding the above signal peptide, the single-chain antibody targeting MUC16,...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a MUC16 targeted chimeric antigen receptor and MUC16 targeted chimeric antigen receptor T cells. The MUC16 targeted chimeric antigen receptor can exclusively target MUC16, a CD27 signal region is used as a costimulatory signal region, amplification of the T cells in bodies of patients is promoted, tumor cells can be efficiently and specifically killed and damaged, and the vitality and the lethality of the cells can be well maintained. The invention further provides a preparation method and application of the MUC16 targeted chimeric antigen receptor T cells.

Description

technical field [0001] The present invention relates to the field of medical biology, in particular to a MUC16-targeted chimeric antigen receptor and chimeric antigen receptor T cells as well as a preparation method and application thereof. Background technique [0002] Ovarian cancer is the most lethal gynecological malignancy in the world. Due to the absence of obvious symptoms and the lack of scientifically credible detection methods, most patients are diagnosed with advanced cancer. Therefore, the cure rate of ovarian cancer is generally low. MUC16 is a transmembrane protein and a member of the mucin (MUC) family. The expression of MUC16 in cervical cancer, pancreatic cancer, and ovarian cancer tissues all increased, and it is an important marker of ovarian cancer, which can be used for early diagnosis of ovarian cancer, detection of treatment effect, and evaluation of tumor recurrence. Studies have shown that MUC16 can inhibit apoptosis by interacting with tumor necro...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/10C12N15/867A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K14/7051C07K16/3092C07K2317/622C07K2319/02C07K2319/03C07K2319/33C12N15/86C12N2510/02C12N2740/15043
Inventor 万晓春刘绿艳李欣唐超
Owner SHENZHEN BINDEBIOTECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com