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Multi-functional micro-fluidic chip and detection method for screening bacterial biofilm inhibitor

A microfluidic chip and inhibitor screening technology, which can be applied in the methods of stress-stimulated microbial growth, biochemical equipment and methods, bioreactors/fermenters for specific purposes, etc., and can solve problems such as limiting the application of microfluidic chips. , to achieve the effect of efficient experimental technology and analytical methods

Active Publication Date: 2019-12-03
CHONGQING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the electrical impedance analysis method has been applied to the research of bacterial biofilm detection, there is no integrated chip and detection technology that integrates microfluidic chip analysis technology, online monitoring of biofilm status and screening of bacterial biofilm inhibitors. The earth limits the application of microfluidic chips in the study of inhibitor-biofilm interaction and inhibitor screening

Method used

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  • Multi-functional micro-fluidic chip and detection method for screening bacterial biofilm inhibitor
  • Multi-functional micro-fluidic chip and detection method for screening bacterial biofilm inhibitor
  • Multi-functional micro-fluidic chip and detection method for screening bacterial biofilm inhibitor

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Effect test

Embodiment 1

[0031] Such as figure 1 As shown, the multifunctional microfluidic chip for biofilm inhibitor screening is composed of two-layer structure with 16 identical unit structures for online in situ detection of bacterial biofilm and high-pass detection of bacterial biofilm inhibitors. volume synchronization filter. The upper layer is a PDMS cover sheet A with microchannels and detection areas, which contains 16 groups of independent structures arranged in upper and lower layers, with 8 groups of independent structures arranged in each of the upper and lower layers; the lower layer is a glass substrate B with integrated microarray electrodes , 16 groups of interdigitated microarray electrodes are integrated on the glass substrate B, each group includes four identical interdigitated electrodes, and the interdigitated electrode area is opposite to the detection area on the upper layer of the PDMS cover sheet A. Each group of microchannels and detection areas above and each group of in...

Embodiment 2

[0037] The above microfluidic chip is produced through the following steps:

[0038] First, the interdigitated microarray electrodes are fabricated on the glass substrate by MEMS standard technology, that is, the substrate of the microfluidic chip is prepared. Before the assembly of the microfluidic chip, the glass substrate is thoroughly cleaned and then dried for use.

[0039] Select polydimethylsiloxane (PDMS) with good biocompatibility, air permeability and light transmission to make the cover sheet containing microchannels. The specific steps are as follows: PDMS and curing agent are poured into the engraved On the SU8 anodic membrane with microstructure, after removing air bubbles, cure at 80°C for 60-80 minutes. After the PDMS cover slip is solidified, peel it off from the SU8 anodic membrane to obtain a PDMS cover slip with micro-channels.

[0040] After the PDMS cover slip and the glass substrate are sterilized, the PDMS cover slip and the glass substrate are bonded t...

Embodiment 3

[0042] Using the above microfluidic chip to detect the effect of tannic acid on E. coli biofilm:

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Abstract

The invention relates to a micro-fluidic chip and detection method for screening a bacterial biofilm inhibitor. The chip consists of substrates for integrating a micro array electrode and a cover plate comprising micro channels, and specifically comprises functional units including sample introducing channels, fluorescent reagent sample introducing channels, biofilm culturing regions, biofilm andmedicine interaction regions, biofilm fluorescent observation regions and the like. Through the adoption of the micro-fluidic chip disclosed by the invention, multiple functions of bacterial biofilm culturing, biofilm fluorescent observation, biofilm impedance monitoring and the like can be realized, the micro-fluidic chip can be applied to biofilm formation process observation and monitoring, medicine and biofilm interaction process on-line in-situ monitoring, bacterial biofilm inhibitor screening and the like; and the multi-functional chip has the advantage of detecting the changes of the biofilm in an in-situ nondestructive real-time online manner, and can analyze the action situation of different medicines on different bacterial biofilms, and then the high-efficiency screening of the biofilm inhibitor is realized.

Description

technical field [0001] The invention belongs to the technical field of cell and bacteria detection, and in particular relates to a multifunctional microfluidic chip for screening bacterial biofilm inhibitors. Background technique [0002] A biofilm is a microbial community composed of bacteria and their own secreted extracellular polymers. Compared with planktonic bacteria, biofilm has strong drug resistance. Once formed, it is difficult to completely remove it, which poses a huge threat to the medical, food and other industries. Screening inhibitors that can prevent the formation of biofilm is the key to solving the biofilm problem. effective way. Screening experiments for biofilm inhibitors are usually carried out in microwell plates, and crystal violet staining is used to evaluate the effect of inhibitors. This method consumes a lot of reagents, the operation steps are cumbersome, and the biofilm staining process and elution process are affected by human factors, result...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/00C12M1/42G01N21/64G01N27/327B01L3/00
CPCB01L3/502707C12M1/00C12M1/42G01N21/6428G01N21/6458G01N21/6486G01N27/3275
Inventor 徐溢刘露陈李苏喜
Owner CHONGQING UNIV
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