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CD30 targeted chimeric antigen receptor, and CD30 targeted chimeric antigen receptor T cell and preparation method and application thereof

A chimeric antigen receptor, CAR-CD30 technology, applied in the field of medical biology, to achieve a highly tissue-specific expression effect

Inactive Publication Date: 2019-12-03
SHENZHEN BINDEBIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no related research on chimeric antigen receptor T cells targeting CD30.

Method used

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  • CD30 targeted chimeric antigen receptor, and CD30 targeted chimeric antigen receptor T cell and preparation method and application thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] A method for preparing chimeric antigen receptor T cells targeting CD30, comprising the following steps:

[0052] (1) Prepare the gene sequence of chimeric antigen receptor CAR-CD30 targeting CD30

[0053] The coding gene sequence of the chimeric antigen receptor CAR-CD30 targeting CD30 is prepared by PCR, and the coding gene of the CAR-CD30 includes the nucleotide sequence shown in SEQ ID NO: 3;

[0054] (2) Construction of pWPXLd-CAR-CD30 recombinant plasmid

[0055] The gene encoding CAR-CD30 was inserted between the BamHI and EcoR I restriction sites of the pWPXLD vector, and behind the EF1α of the pWPXLD vector, EF1α was used as the promoter. When the CAR-CD30 coding gene is inserted into the pWPXLD vector, a start codon (such as ATG) is added to the 5' end of the CAR-CD30 coding gene to connect to the BamH I restriction site in the pWPXLD vector, and the 3' end A stop codon (such as TAA) is also added to connect with the EcoR I restriction site in the pWPXLD vec...

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PUM

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Abstract

The invention provides a CD30 targeted chimeric antigen receptor CAR-CD30. The CAR-CD30 comprises an amino acid sequence as shown in SEQID NO:1. The invention further provides a chimeric antigen receptor T cell including the CAR-CD30. The CD30 targeted chimeric antigen receptor CAR-CD30 can specifically target CD30 positive tumor cells and activate T cells to exert cellular immunity effects to realize efficient and specific killing on the CD30 positive tumor cells, has persistent cell viability and lethality, and cannot damage normal cells. The invention further provides a preparation method of the CD30 targeted chimeric antigen receptor T cell, a recombinant virus vector having CAR-CD30 coding genes, and the like.

Description

technical field [0001] The invention relates to the field of medical biology, in particular to a CD30-targeting chimeric antigen receptor, a chimeric antigen receptor T cell and a preparation method and application thereof. Background technique [0002] Malignant tumors (cancer) have become the main culprit that threatens human life, and the incidence is on the rise. For example, mature T-cell lymphoma (MTCL) is a rare and extremely aggressive type of cancer for which standard chemotherapy regimens have remained unchanged for decades. CD30 protein is a well-defined biomarker for classical Hodgkin's lymphoma (HL) and systemic anaplastic large cell lymphoma (sALCL), which is expressed in various types of non-Hodgkin's lymphoma (nHL) and Hodgkin's lymphoma Gold lymphoma (HL), CD30 can be selected as a tumor target for the treatment of MTCL. [0003] CAR-T (Chimeric Antigen Receptor T-cell) technology is a new type of cell therapy, which is to reinfuse CAR-modified T cells int...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K19/00C12N5/10C12N15/867A61K35/17A61P35/00
CPCA61K35/17A61P35/00C07K14/7051C07K16/2878C07K2319/00C12N15/86C12N2740/15043
Inventor 万晓春唐超
Owner SHENZHEN BINDEBIOTECH CO LTD
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