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Porous cuprous oxide nano crystals, and preparation method and application thereof

A technology of cuprous oxide and nanocrystals, applied in the field of anti-tumor nanomaterials, achieving the effects of low cytotoxicity, low price, and simple preparation methods

Inactive Publication Date: 2019-12-03
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the toxicity of copper ions themselves also has a strong killing effect on normal cells.

Method used

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  • Porous cuprous oxide nano crystals, and preparation method and application thereof
  • Porous cuprous oxide nano crystals, and preparation method and application thereof
  • Porous cuprous oxide nano crystals, and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0021] Preparation of porous cuprous oxide nanocrystals: 5 g of polyethylene glycol (molecular weight: 10 000 kDa) was ultrasonically dissolved in 100 mL of 10 mM copper acetate monohydrate aqueous solution. 6 M sodium hydroxide aqueous solution (0.5 mL) was added at a rate of 8.0 μL / s, followed by reaction at room temperature (25 °C) for 30 min. After the reaction, 1 M ascorbic acid aqueous solution (2 mL) was added at a rate of 6.5 μL / s, and the mixture was continued to react for 30 min under nitrogen protection after the addition. Subsequently, the mixture was centrifuged and washed three times with deionized water and ethanol, respectively. The centrifuged solid was dried in a vacuum oven for 24 h and stored under nitrogen.

[0022] The transmission electron microscope image of the porous cuprous oxide nanocrystals prepared by this method is as follows: figure 1 , it can be seen from the transmission electron microscope that the prepared porous cuprous oxide nanocrystals...

Embodiment 2

[0024] Application of porous cuprous oxide nanocrystals in inhibiting tumor cells: Tumor cells, human lung cancer cells A549 or normal cells, mouse fibroblast L929 cells were seeded in 96-well plates and incubated at 37 °C, 5% CO 2 Incubate overnight in the incubator. Subsequently, the medium was replaced with a suspension containing different concentrations of cuprous oxide nanocrystals, and the culture was continued for 24 h. Next, the cell viability was detected by the MTT (thiazolium) method.

[0025] From Figure 4 and Figure 5 It can be seen that the prepared porous cuprous oxide nanocrystals have obvious killing effect on tumor cells, IC 50 It was 36.34 μg / mL. It is less toxic to normal cells. This may be due to the weak acidic microenvironment of tumor cells has a strong degradation effect on cuprous oxide nanocrystals, while the neutral environment of normal cells degrades polyethylene glycol-protected cuprous oxide nanocrystals very slowly.

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Abstract

The invention relates to porous cuprous oxide nano crystals, and a preparation method and application thereof. The preparation method comprises following steps: ultrasonically dissolving polyethyleneglycol in a copper acetate monohydrate aqueous solution, adding a sodium hydroxide aqueous solution, adding an ascorbic acid aqueous solution after the reaction is finished, and continuously reactingan obtained mixed solution under the protection of nitrogen after the addition is finished; and then centrifuging the mixed solution, washing with deionized water and ethanol respectively, drying a solid obtained by centrifugation in a vacuum drying oven, and storing in nitrogen. The preparation method of the porous cuprous oxide nano crystals is simple; the raw materials are widely available andcheap. The prepared cuprous oxide nano crystals have an obvious pore channel structures. The prepared porous cuprous oxide nano crystals have a remarkable inhibition effect on tumor cells A549, and have low cytotoxicity on normal cell mouse fibroblasts L929.

Description

technical field [0001] The invention belongs to the field of anti-tumor nanomaterials, and relates to a porous cuprous oxide nanocrystal, a preparation method thereof and an application in inhibiting human lung cancer cell A549. [0002] technical background [0003] Cancer is also known as malignant tumor, and its morbidity and mortality are currently among the top three. Among them, men are mainly lung cancer, while women are breast cancer. Therefore, cancer is undoubtedly the "super killer" hidden around us. Chemotherapy is currently the main treatment for cancer, but it generally has disadvantages such as poor solubility, non-specificity, and severe side effects, which greatly limit the use of chemotherapy drugs. With the development of science and technology, nanomaterials, as an emerging science and technology, have been proved to have the effect of replacing chemotherapy drugs. The size of such nanoparticles is generally around 1-100 nm, which can identify tumor c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01G3/02A61K33/34A61P35/00B82Y30/00
CPCA61K33/34A61P35/00B82Y30/00C01G3/02C01P2004/64
Inventor 孙柏旺吴鸿帅陈方慧高志国
Owner SOUTHEAST UNIV
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