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Preparation method and applications of Taxus Chinensis polysaccharide with hyperuricemia reducing effect

A technology of yew and uric acid, which is applied in the field of medicine, can solve the problems affecting the treatment of hyperuricemia, many adverse reactions, poor tolerance of patients, etc., and achieve the effects of improving extraction rate and purity, increasing utilization rate, and reducing costs

Pending Publication Date: 2019-11-29
武汉紫杉红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These drugs have many adverse reactions and are poorly tolerated by patients, which greatly affects the treatment of hyperuricemia

Method used

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  • Preparation method and applications of Taxus Chinensis polysaccharide with hyperuricemia reducing effect
  • Preparation method and applications of Taxus Chinensis polysaccharide with hyperuricemia reducing effect
  • Preparation method and applications of Taxus Chinensis polysaccharide with hyperuricemia reducing effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1 Exploration of the preparation process and pretreatment method of the total polysaccharide of Taxus chinensis

[0036] The inventor pretreated the yew powder with 80% ethanol before water extraction and alcohol precipitation, in order to remove the remaining fat-soluble compounds and some monosaccharides in the medicinal material, so as to improve the extraction efficiency of the medicinal material polysaccharide in step B and increase the yield. The inventor compared four kinds of solid-liquid ratios 1:5, 1:10, 1:15, and 1:20 respectively, and found that when the solid-liquid ratio was 1:5, the impurity removal was incomplete, while the solid-liquid ratio was 1:15 and 1:15. :20 Although the effect is very good, it will increase the production cost, so the preferred material-to-liquid ratio is 1:10.

Embodiment 2

[0037] Example 2 Exploration of the preparation process of total polysaccharides of Taxus chinensis by water extraction and alcohol precipitation

[0038] Water extraction and alcohol precipitation is currently the most commonly used method for extracting polysaccharides from medicinal materials. The methods are similar, but the extraction process needs to be verified experimentally to improve production efficiency and reduce costs while maintaining the extraction rate. The inventor used the ratio of solid to liquid 1:5, 1:8, 1:10, 1:15 and extracted 1, 2, and 3 times, and took the extraction rate of the extract as a reference standard to explore the extraction process and confirm the optimal extraction process. : Extract twice, the first time the ratio of solid to liquid is 1:10, the second time is 1:8.

Embodiment 3

[0039] Example 3 Exploration of the preparation process and washing method of the total polysaccharide of Taxus chinensis

[0040] In order to ensure that the washing step can effectively remove impurities and reduce the loss of polysaccharides during washing, the inventor investigated the best washing method by observing the color change of the washing liquid during the washing process and the polysaccharide content test method in the washing liquid, and confirmed the washing process: adding absolute ethanol The volumes of , acetone, and ethyl acetate are all 0.1 times (mass / volume) of the southern yew feeding amount.

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PUM

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Abstract

The invention discloses a preparation method and applications of Taxus Chinensis polysaccharide with hyperuricemia reducing effect. The preparation method specifically comprises: A) pretreatment; B) water extraction and alcohol precipitation; C) washing; D) protein removing; E) dialysis; and F) freeze drying. The invention further discloses applications of the Taxus Chinensis polysaccharide prepared by the method in hyperuricemia reduction, and a medicament used for reducing hyperuricemia and prepared by the Taxus Chinensis polysaccharide prepared by the method. According to the present invention, the method has characteristics of low Taxus Chinensis polysaccharide extracting cost, high extraction rate and high product purity, and the obtained Taxus Chinensis polysaccharide has hyperuricemia reducing effect, and can protect kidney.

Description

technical field [0001] The invention belongs to the technical field of medicines, and in particular relates to a preparation method and application of yew polysaccharide with uric acid-lowering effect. Background technique [0002] Hyperuricemia refers to a body state in which the concentration of uric acid in the blood exceeds the normal range. It is generally believed that when the concentration of uric acid in the blood of men is greater than 400 μmol / L, and that of women is greater than 360 μmol / L, hyperuricemia should be considered. blood disease. Recent studies have shown that hyperuricemia is not only the most important biochemical basis of gout, but also an important cause of hypertension, hyperlipidemia, atherosclerosis, obesity, insulin resistance, metabolic syndrome, cardiovascular disease, and death. Factors have become one of the major metabolic diseases that threaten human health. [0003] At present, the chemical drugs for the clinical treatment of hyperuric...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K31/715A61P19/06
CPCA61P19/06A61K31/715C08B37/0003
Inventor 李晓王赞
Owner 武汉紫杉红生物科技有限公司
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