Method for silencing TRV-based virus induced primula malacoides genus plant gene
A technology of gene silencing and primrose, applied in the field of plant genetic engineering, can solve problems such as the research on the function of genes limiting the S site, and achieve the effects of short research period, high phenotype efficiency and simple method
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Embodiment 1
[0056] The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention. Unless otherwise specified, the examples are all in accordance with conventional experimental conditions, such as Sambrook et al. Molecular Cloning Experiment Manual (Sambrook J & Russell DW, Molecular Cloning: a Laboratory Manual, 2001), or in accordance with the conditions suggested by the manufacturer's instructions. Example 1 TRV-based virus-induced method for gene silencing of Primula miltiorrhiza
[0057] 1. Acquisition of experimental materials: Seeds are sown on MS medium, and the seedlings are cultivated to 6-9 true leaves before being bottled, and the seedlings are cultivated in an artificial climate chamber after being out of the bottle for subsequent use. Culture conditions: light 300μmol·m -2 ·s -1 , 16 hours of light, 8 hours of darkness, daytime temperature of 22°C, darkness of 18°C, and humidity of 60%.
[0058] 2. Rec...
Embodiment 2
[0082] Example 2 Using VIGS to analyze the function of the auxin export protein gene in Primula miltiorrhiza
[0083] Auxin is involved in regulating various processes in plant growth and development, such as cell division and cell elongation. Physiological pleiotropy was exhibited at the tissue, organ and whole plant levels. The transport of auxin between cells depends on the carrier located in the cell membrane. Auxin export protein PIN-FORMED (PIN) is an important class of proteins that transport auxin to the extracellular transmembrane. It has been reported that silencing the TaPIN1 gene in wheat leads to dwarf plants (Kanwardeep, 2011), while the PtPIN8 gene is negatively correlated with the root hair length of Citrus citrus (Liu et al., 2108). The PIN gene may affect the elongation or division of cells by affecting the transport and redistribution of auxin, and ultimately affect the height or length of the whole plant or organ. In the early stage of the present invent...
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