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Preparation method of composite microbial agent for harmless treatment of fecal sewage

A compound microbial inoculum and harmless treatment technology, which is applied in the field of compound microbial inoculum preparation, can solve the problems of single use effect, poor stability, short survival time, etc., and achieves strong practicability, stable performance and short production cycle. Effect

Inactive Publication Date: 2019-11-15
陈玉亭
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the microbial agents suitable for the improvement of urban and rural ecological environments and the needs of agricultural production are based on a single strain. A single strain shows disadvantages such as poor stability, single use effect, and short survival time, which can no longer adapt to the development of modern agriculture. needs

Method used

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  • Preparation method of composite microbial agent for harmless treatment of fecal sewage

Examples

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Comparison scheme
Effect test

Embodiment 1

[0024] Example 1 A preparation method for a compound microbial agent for harmless treatment of manure, refer to figure 1 As shown, the steps are as follows:

[0025] (1) Prepare three nutrient solutions, 50 liters each, label them separately, and prepare them for use after sterilization; the nutrient solution is composed of water plus 8% sugar;

[0026] (2) Inoculate microbial bacteria A into one of the nutrient solutions in step (1), and place it at a temperature of 28-30°C for 48 hours of aerobic culture in the dark, and set aside; among them, microbial bacteria A is an aerobic beneficial microorganism bacteria;

[0027] (3) Inoculate microbial bacteria B into another nutrient solution in step (1), place it at 28-30°C for 48 hours of aerobic culture in the dark, and set aside; wherein microbial bacteria B is an aerobic beneficial microbial fungus;

[0028] (4) Inoculate microbial bacteria C into the remaining part of the nutrient solution in step (1), and place it at a tem...

Embodiment 2

[0030] Example 2 A preparation method for a compound microbial agent for the harmless treatment of feces, refer to figure 1 As shown, the steps are as follows:

[0031] (1) Prepare three nutrient solutions, 50 liters each, label them separately, and prepare them for use after sterilization; the nutrient solution is composed of water plus 8% sugar;

[0032] (2) Inoculate microbial bacteria A into one of the nutrient solutions in step (1), and place it at a temperature of 28-30°C for 48 hours of aerobic culture in the dark, and set aside; wherein, microbial bacteria A is Bacillus subtilis;

[0033] (3) Inoculate microbial bacteria B into another nutrient solution in step (1), place it at 28-30°C for 48 hours of aerobic culture in the dark, and set aside; wherein microbial bacteria B is Saccharomyces cerevisiae;

[0034] (4) Inoculate microbial bacteria C into the remaining part of the nutrient solution in step (1), and place it at a temperature of 28-30°C for 48 hours of dark a...

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Abstract

The invention discloses a preparation method of a composite microbial agent for harmless treatment of fecal sewage. The preparation method includes the following steps that three nutrient solutions are prepared, each nutrient solution is 50 liters, and the three nutrient solutions are marked separately and sterilized for use; a microbial bacterium A is inoculated into one nutrient solution, and light-free aerobic culture is conducted for 48 hours; a microbial bacterium B is inoculated into another nutrient solution, and light-free aerobic culture is conducted for 48 hours; a microbial bacterium C is inoculated into the remaining nutrient solution, the remaining nutrient solution is placed at the temperature of 28-30 DEG C, and light-free anaerobic culture is conducted for 48 hours; after 48 hours, the prepared bacterial solutions are mixed in a closed container, the closed container is placed at the temperature of 28-30 DEG C, light-free culture is conducted for 7-10 days, and gas discharge is conducted every 4-5 hours; when the pH is smaller or equal to 4.0, a finished product is prepared. The provided composite microbial agent has a simple preparation process, wide application range, stable performance, obvious effect, no toxic or side effects, and high practicability.

Description

technical field [0001] The invention relates to a preparation method of a compound microbial bacterial agent for harmless treatment of manure. Background technique [0002] With the continuous improvement of people's living standards, the improvement of the ecological environment and the development of green agriculture have been put on the agenda of the party and the country. The application of microbial technology is more popular in improving the modern ecological environment and in modern agricultural production. At present, most of the microbial agents suitable for the improvement of urban and rural ecological environments and the needs of agricultural production are based on a single strain. A single strain shows disadvantages such as poor stability, single use effect, and short survival time, which can no longer adapt to the development of modern agriculture. needs. The scientific compounding of several beneficial bacteria effectively solves the above disadvantages. ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/18C05F15/00C05F17/00C12R1/125C12R1/23C12R1/865
CPCC05F3/00C05F17/00C12N1/18C12N1/20C05F3/04C05F11/08Y02W30/40
Inventor 陈玉亭
Owner 陈玉亭
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