Sodium dithionite colorimetric fluorescent probe as well as preparation method and application thereof

A technology of sodium dithionite and fluorescent molecular probes, which is applied in the field of analysis and detection, can solve problems such as inability to realize direct and rapid detection, being easily interfered by other substances, and less research on content detection methods, and achieves good social value and application prospects. Improved specificity and sensitivity, high yield

Active Publication Date: 2019-11-15
CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] However, most of the detection methods currently available are for Na2S2O 4 is used as an industrial reducing agent, and as a food additive, research on the detection method of Na2S2O4 content There are relatively few, and the existing detection methods have the following points to be improved, and the direct and rapid detection of Na2S2O4 cannot be achieved Detection, susceptible to interference from other substances, inconvenient to operate, time-consuming, etc.
Therefore, there is a lack of research on a method with high selectivity and the ability to quickly and directly detect Na2S2O4

Method used

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  • Sodium dithionite colorimetric fluorescent probe as well as preparation method and application thereof
  • Sodium dithionite colorimetric fluorescent probe as well as preparation method and application thereof
  • Sodium dithionite colorimetric fluorescent probe as well as preparation method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0022] Embodiment 1: the synthesis of sodium dithionite colorimetric fluorescent molecular probe, the synthetic route is as follows figure 1 As shown in (B), the basic operation process is as follows.

[0023] (1) Synthesis of anthocyanin-like fluorescent dyes: N,N-diethyl-4-amino-2-hydroxy-benzaldehyde (200mg, 1.03mmol) and 6-amino-3,4-dihydro-1 (2H)-Naphthalenone (185 mg, 1.13 mmol) was sequentially added to a flask (50 mL) filled with concentrated sulfuric acid (5 mL), heated to 90 °C under nitrogen protection, and reacted for 5 hours. After the reaction was completed and cooled, perchloric acid (70%, 7 mL) was added in an ice bath, and the extractant (CH 2 Cl 2 / MeOH, 20:1) to extract the product. Use 30 mL of solution for extraction each time, collect the organic phase, and repeat the extraction four times. After the extraction is completed, add excess anhydrous magnesium sulfate to the collected organic phase and dry it for 1 hour, remove the magnesium sulfate by suct...

Embodiment 2

[0026] Example 2: Feasibility verification of sodium dithionite colorimetric fluorescent molecular probe

[0027] In order to verify whether the obtained target probe mechanism has the expected ideal function, a preliminary experiment was carried out. Such as figure 2 As shown in (A), the sodium dithionite colorimetric fluorescent molecular probe has a strong absorption peak at 620nm. 2 S 2 o 4 (10mM), the absorption peak is obviously blue-shifted, and the probe and Na in the absorption cell 2 S 2 o 4 Before and after the response, the color changed from blue to red obviously. As shown in Figure (B), under the excitation of 570nm, the sodium dithionite colorimetric fluorescent molecular probe exhibited a very small fluorescence emission peak, but adding Na 2 S 2 o 4 After (10mM), the fluorescence emission intensity of the probe increased significantly, indicating that the probe is sensitive to Na 2 S 2 o 4 response with high sensitivity. And at the same time with...

Embodiment 3

[0028] Example 3: Optimization of response conditions for sodium dithionite colorimetric fluorescent molecular probes

[0029] In order to optimize the best detection system, a common solvent: acetonitrile (CH 3 CN), N,N-dimethylformamide (DMF), tetrahydrofuran (THF), dimethyl sulfoxide (DMSO), ethanol (EtOH), methanol (MeOH) screening experiments. 2µM probe at 620nm in different solvents and addition of 10mM Na 2 S 2 o 4 After the fluorescence response intensity ratio, given by image 3 It can be seen that when EtOH is the solvent, the probe and Na 2 S 2 o 4 Response is best.

[0030] Inorganic salt Na 2 S 2 o 4 It is easily soluble in water and not easily soluble in EtOH, so the experiment next explored the effect of the ratio of ethanol to water on the response of the probe. obtained in EtOH / H 2 O different from 2µM probe at 620nm and addition of 10mM Na 2 S 2 o 4 The fluorescence response intensity ratio after. Depend on Figure 4 It can be seen that under...

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Abstract

The invention provides a colorimetric fluorescent bifunctional molecular probe, a preparation method of the probe and an application of the probe in detection of illegal additivesodium dithionite in food samples, and belongs to the technical field of analytical chemistry. The probe has a molecular formula of C29H31N4O<+> and a structural formula represented by a formula I shown in the description.The sodium dithionite colorimetric fluorescent bifunctional molecular probe designed by the invention has a simple preparation method and a mature synthetic route, can realize direct detection of thesodium dithionite, and has the characteristics of good selectivity, a fast detection speed, high sensitivity and obvious color change; and the method provided by the invention has original innovationand good social value, and is expected to be applied to the detection of illegal additives such as the sodium dithionite in food.

Description

technical field [0001] The invention belongs to the field of analysis and detection, and in particular relates to the construction of a colorimetric fluorescent double-functional organic small molecule probe and a new method for detecting and analyzing sodium dithionite, an illegal additive in food, using the same. Background technique [0002] Food additives refer to a class of natural or chemically synthesized substances used to prevent food deterioration and prolong food storage, facilitate food processing, maintain and improve food nutritional value, and improve food sensory properties. The state has strict regulations on the use of additives, and illegal additives are not allowed. Sodium dithionite (Na 2 S 2 o 4 • 2H 2 O) Commonly known as sodium hydrosulfite, it is a white sandy crystal or light yellow powder chemical product. It is an industrial reducing bleaching agent. It is a chemical substance that is very harmful to the human body and is not an edible substan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C09K11/06C07D311/78G01N21/64
CPCC09K11/06C07D311/78G01N21/643C09K2211/1007C09K2211/1088
Inventor 杨盛余熙子杨荣华文锡旦
Owner CHANGSHA UNIVERSITY OF SCIENCE AND TECHNOLOGY
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