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Radiation-induced skin injury control medicine

A technology of radioactive skin and drugs, applied in skin diseases, drug combinations, pharmaceutical formulations, etc., to achieve the effects of accelerating healing, promoting migration, promoting cell proliferation and tissue regeneration

Pending Publication Date: 2019-11-15
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The effect and mechanism of Wnt / β-catenin signaling pathway on mammalian skin tissue radiation damage have not been reported yet

Method used

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  • Radiation-induced skin injury control medicine
  • Radiation-induced skin injury control medicine
  • Radiation-induced skin injury control medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Example 1 Activation of skin cell WS1 by Wnt3a, verification of β-catenin signal transduction pathway in HaCaT

[0029] Human skin fibroblasts WS1 in exponential growth phase and human immortalized epithelial keratinocytes HaCaT were inoculated on a glass-bottomed cell culture dish, and the cells were irradiated with X-rays after the cells adhered to the wall, and Wnt3a protein (1 μg / mL ) and β-catenin pathway inhibitor XAV-939 (50 μmol / L), at 37°C, 5% CO 2 Continue culturing for 24 h in the incubator. The fluorescence intensity and localization of β-catenin in cells were observed by confocal microscope. Such as figure 1 As shown, the results showed that: in WS1 and HaCaT cells, Wnt3a significantly enhanced the fluorescence intensity of β-catenin in the cells, and made the accumulation of β-catenin into the nucleus. Wnt3a can activate the β-catenin signal transduction pathway in skin cells.

Embodiment 2

[0030] Example 2 The experiment of activating the β-catenin signal transduction pathway to reduce the free radicals caused by radiation

[0031] Human skin fibroblasts WS1 in the exponential growth phase and human immortalized epithelial keratinocytes HaCaT were inoculated in 96-well plates, and the absorbed doses of 0, 5, 10, and 20 Gy X-rays were irradiated after the cells adhered to the wall. Then add Wnt3a and XAV-939, at 37°C, 5% CO 2 Continue culturing for 24 h in the incubator. Each group had 4 parallel samples, and the experiment was repeated 3 times. Aspirate the medium, add 1 mL of diluted DCFH-DA to each well (dilute DCFH-DA with serum-free medium according to 1:1000, so that the final concentration is 10 μmol / L), and incubate in the incubator for 20 min. Wash three times with serum-free medium to fully remove DCFH-DA that did not enter the cells. Detection by microplate reader. Such as figure 2As shown, the results showed that: Wnt3a activated β-catenin signa...

Embodiment 3

[0032] Example 3 Activating the β-catenin signal transduction pathway to inhibit the apoptosis of skin cells by ionizing radiation

[0033] Human skin fibroblasts WS1 in exponential growth phase and human immortalized epithelial keratinocytes HaCaT were inoculated in 6-well plates. After the cells adhered to the wall, they were irradiated with different absorbed doses of X-rays, and then Wnt3a and XAV were added. -939 at 37°C, 5% CO 2 Continue culturing for 24 h in the incubator. Three parallel samples were set up in each group, and the experiment was repeated 3 times. Cells were collected, half life and death were set, stained with 7AAD and PE, and the apoptosis rate of the two cells was detected by flow cytometry. Such as image 3 As shown, the results showed that: Wnt3a activated β-catenin signal transduction pathway can significantly inhibit the apoptosis of skin cells induced by ionizing radiation.

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Abstract

The invention provides a radiation-induced skin injury control medicine for activating a beta-catenin signal transduction route. The medicine adopts Wnt3a protein as a main effective component to activate the beta-catenin signal transduction route, and has important control effects on radiation-induced skin injury of human skin cells and mouse skin tissue. The oxidation capacity and the apoptosisresistance are reinforced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a drug for activating the β-catenin signal transduction pathway to treat radiation skin damage. Background technique [0002] With the development of radiation physics, computer technology and medical imaging technology, the radiotherapy technology for tumors has developed rapidly, and many advanced radiotherapy technologies such as IGRT, IMRT, 3D-CRT, etc. Normal tissue will still inevitably be irradiated. In addition, the potential for nuclear accidents remains. Radiation-induced skin injury is a common complication after tumor radiotherapy, radioactive nuclear accidents, and pretreatment for bone marrow transplantation. The main causes include nuclear radiation accidents, tumor radiotherapy, and occupational exposure. For example, in the Chernobyl accident in the former Soviet Union in 1986, more than 50% of the irradiated patients had different degrees of radiation skin damage....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K38/16A61P17/16A61P17/18
CPCA61K38/16A61P17/16A61P17/18
Inventor 曹建平曹津铭张舒羽朱巍
Owner SUZHOU UNIV
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