In-vitro neural network construction system based on robotization

Abstract

The invention discloses an in-vitro neural network construction system based on robotization. Neuron tissues to be constructed are arranged in a biological culture dish. A laser subsystem comprises alaser source, a laser conduction device and a focusing objective lens. The laser source is connected with the laser conduction device through an energy supply cable and used for conducting laser energy, the focusing objective lens is connected with the laser conduction device through a laser optical fiber, the focusing objective lens is arranged above the biological culture dish, and the focusingobjective lens is used for focusing conducted laser. The focusing objective lens is fixed on the displacement control platform, the biological culture dish is arranged below the focusing objective lens by controlling the displacement control platform to move, and laser ablation or optical stimulation is carried out on target point cells or tissues in the biological culture dish. According to the method, specific activation can be carried out on a single neuron, meanwhile, responses of other neurons in the neural network can be recorded, integration is easy, the integration cost is low, and very high flexibility and operability are achieved.

Description

technical field [0001] The invention belongs to the technical field of bioengineering and mechanical automation control and brain-inspired technology research methods, and specifically relates to a robot-based in vitro neural network construction system, which can be used to explore the neuron network structure connection and information transmission characteristics under bionic conditions. Provide algorithm structure and transfer function model for brain-inspired simulation calculations, and explore new robot control theory research based on brain-inspired control. Background technique [0002] In recent years, a variety of new technologies have been applied in the field of neuroscience research, especially the application of laser technology in the fields of fluorescent labeling of nerve cells, optogenetic neuron activation, fluorescent recording of neuronal calcium activity, and laser bleaching. The development of neuroscience provides an effective means to accelerate the...

AI Technical Summary

Problems solved by technology

However, the patch clamp technique has high requirements for equipment cost, learning cost and space. In addition, patch clamp recording cannot perform high-throughput simultaneous recording of multiple neurons.

Images