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Complex microbial agent, and application of complex microbial agent to kitchen waste fermentation for preparation of organic liquid fertilizer

A technology of composite bacterial agent and kitchen waste, applied in the direction of organic fertilizers, bacteria, fungi, etc., can solve the problems of high temperature, labor, energy, etc., and achieve remarkable results

Active Publication Date: 2019-11-12
上海利蒙生态环境科技(集团)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aerobic composting is one of the common methods for food waste treatment and resource utilization, but it requires aerobic microorganisms, turning over the compost during the process, high labor and energy costs, and high temperature during the fermentation process, so yeast cannot be used Medium and low temperature bacterial fermentation

Method used

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  • Complex microbial agent, and application of complex microbial agent to kitchen waste fermentation for preparation of organic liquid fertilizer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Composite microbial bacterial agent to treat kitchen waste and make liquid organic fertilizer

[0039] 1. Bacteria culture

[0040] (1) Strain activation:

[0041] Yarrowia lipolytica (Yarrowia lipolytica) CICC 32187, Portuguese clavispora lusitaniae (Clavispora lusitaniae) CICC 32908, Candida utilis (Candida utilis) CICC 1768 were inoculated into YPD solid medium respectively, and cultured at 30°C for 2 days , to obtain the activated cells respectively;

[0042] Lactobacillus plantarum (Lactobacillus plantarum) CICC 21801 was inoculated into the MRS solid medium and cultured at 35°C for 4 days to obtain activated bacteria.

[0043] The composition of YPD liquid medium: glucose 20g / L, peptone 20g / L, yeast powder 10g / L, solvent is deionized water, pH is natural; YPD solid medium is to add agar powder 15g / L to YPD liquid medium. The medium was sterilized at 121 °C for 20 min.

[0044] MSR liquid medium composition: peptone 10g / L, beef extract 10g / L, yeast ex...

Embodiment 2

[0055] Embodiment 2, compound microbial agent fermented food waste to make organic liquid fertilizer

[0056] 1, the composite bacterial agent is prepared according to the method of Example 1

[0057] Compound bacterial agent volume ratio: OD 600 20 parts of Yarrowia lipolytica suspension with a value of 0.6, OD 600 Corynespora portuguese suspension 15 parts with a value of 0.6, OD 600 25 parts of Candida utilis suspension with a value of 0.6, OD 600 40 parts of Lactobacillus plantarum suspension with a value of 0.4.

[0058] 2. Disposal of kitchen waste

[0059] The preparation of the kitchen waste slurry was the same as in Example 1, and the composite bacterial agent in step 1 was added in an amount of 1.5 L of the composite bacterial agent per ton of the slurry.

[0060] Add the slurry and the compound bacterial agent to a plastic bucket (food grade) with a screw-top lid for fermentation, and the lid of the container should not be screwed too tightly to keep it in a br...

Embodiment 3

[0065] Example 3, Comparison of application effect of two kinds of bacteria combination and four kinds of bacteria combination fermentation agent to process the liquid organic fertilizer nutrient solution produced by kitchen waste to promote tomato growth

[0066] 1, single bacterium culture method is prepared by embodiment 1 method

[0067] The volume ratio of two kinds of compound bacterial agents is designed with the combination of yeast + lactic acid bacteria, three combinations:

[0068] A: OD 600 20 parts of Yarrowia lipolytica suspension with a value of 0.6, OD 600 40 parts of Lactobacillus plantarum suspension with a value of 0.4.

[0069] B: OD 600 Corynespora portuguese suspension 15 parts with a value of 0.6, OD 600 40 parts of plantarum lactobacillus suspension with a value of 0.4.

[0070] C:OD 600 25 parts of Candida utilis suspension with a value of 0.6, OD 600 40 parts of Lactobacillus plantarum suspension with a value of 0.4.

[0071] D: OD 600 20 par...

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Abstract

The invention discloses a complex microbial agent, and application of the complex microbial agent to kitchen waste fermentation for preparation of organic liquid fertilizer. The complex microbial agent is prepared from the following bacterium suspensions in parts by volume: 20-30 parts of yarrowia lipolytica bacteria, 10-20 parts of clavispora lusitaniae bacteria, 15-25 parts of candida utilis bacteria, and 35-55 parts of lactobacillus plantarum bacteria. After kitchen waste is subjected to strain combination and fermentation treatment, the content of amino acid nitrogen is increased to be 4.89% from 1.86% and increased remarkably compared with the content before fermentation, the complex microbial agent is rich in various beneficial microorganisms such as microzyme and lactic acid bacteria and nutrients secreted by the beneficial microorganisms, by serving as the liquid fertilizer, absorption of plants is promoted, and the nutrient level is improved.

Description

[0001] (1) Technical field [0002] The invention relates to the field of environmental protection treatment of organic waste such as kitchen waste and discarded food, in particular to the use of several microorganisms that secrete different enzymes to degrade the food waste substrate in a targeted manner, and the degraded products can become high-quality fertilizers , used in agricultural production, landscaping, etc. [0003] (2) Background technology [0004] As the hazards of food waste become increasingly prominent, how to effectively recycle, reduce, and harmlessly treat food waste is one of the key issues. [0005] Food waste is a rich organic resource. Food waste in my country mainly includes food residues such as rice and flour, vegetables, vegetable oil, animal oil, meat bones, fish bones, etc. The physical state is a solid-liquid mixture and is quite viscous; the chemical composition is complex, mainly including water, protein, and lipids. , starch, cellulose and in...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12N1/20C05F9/04C12R1/25C12R1/72C12R1/645
CPCC05F9/04C12N1/16C12N1/20Y02A40/20
Inventor 朱廷恒汪琨王渭霞
Owner 上海利蒙生态环境科技(集团)有限公司
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