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The method of rearing South American tomato leafminer with one head

A technology for leaf miners and tomato, applied in the field of insect breeding, can solve the problems of early shedding, limited breeding scale, poor observation effect, etc., and achieve the effects of avoiding larvae escape, long fresh-keeping time and good breeding effect.

Active Publication Date: 2021-05-25
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, there are following defects in the first and second methods: 1, because the weight of the culture vessel is all attached to a single leaf, it often causes the blade to break off and fall off in advance, and the insects have not yet fully developed the leaves; 2, because the culture vessel After being fixed, it cannot be rotated at will, and only a certain angle of the blade can be observed, which is not conducive to observation, especially to the observation of eggs and first egg larvae; when observed through a culture vessel containing spun yarn or spun yarn bag, the vision is blurred, especially for latent larvae. The observation effect of leaf pests (such as leaf miners, leaf miners, etc.) is not good; 3. Due to the small space and limited leaf area, larvae will also leave the caged area through sneaking passages, making it impossible to raise and observe single heads. Continued, the experiment was forced to terminate; 4. Potted plants take up a lot of space and can only be carried out in fields, greenhouses or small artificial climate chambers. The environmental conditions in fields and greenhouses are difficult to control, while small artificial climate chambers can control Temperature and humidity, but the scale of breeding is very limited. For example, a small artificial climate chamber with a volume of 450L can only place up to 4 potted plants. One plant is counted as 10 true leaves, and only about 40 plants can be kept and observed at the same time. insect
[0006] The defect of the third method is that the culture vessel cannot be placed sideways or upside down for observation (sideways or upside down will cause hydroponic water to pour), resulting in unclear identification of insect age and state, and inaccurate observation results, especially for leaf miners. The observation results of insects, eggs (about 0.3mm) and newly hatched larvae (body length about 0.5mm) are inaccurate; the breeding scale of this method is also difficult to expand, for example, a small artificial climate chamber with a volume of 450L can only Observe and feed 40-50 insects at the same time

Method used

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  • The method of rearing South American tomato leafminer with one head
  • The method of rearing South American tomato leafminer with one head
  • The method of rearing South American tomato leafminer with one head

Examples

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Effect test

Embodiment 1

[0029] Such as figure 1 Shown, the present invention is used to raise the shape of the transparent rearing box that South American tomato leaf miner is used and is cylindrical, and described transparent rearing box comprises upper rearing box 1 and lower rearing box 2 stacked up and down, and described upper rearing box 1 is equal to the diameter and height of the lower rearing box 2, wherein the diameter and height are 85mm and 13mm respectively. The bottom surfaces of the upper rearing box 1 and the lower rearing box 2 are all provided with openings, and the openings of the two boxes are docked with the openings during use to form a cylindrical breeding box with a diameter of 85mm and a height of 26mm, which is ready for use.

[0030] Collect clean and fresh-cut tomato leaves, which contain 1 to 3 leaflets, and the petiole is 45 to 50 mm long. Cut the petiole into an oblique section with a sharp knife to facilitate water absorption. Wrap the petiole with saturated absorbent...

Embodiment 2

[0032] Embodiment 2 investigates the raising situation of South American tomato leaf miner

[0033] Taking the South American tomato leafminer as the experimental object and fresh tomato as the host plant, the tomato leaves with eggs (egg period is about 12h) are kept fresh and put into a cylindrical transparent plastic breeding box, and the egg growth rate is observed and recorded once a day. Status and color changes; after the larvae hatch, observe and record the developmental status of the larvae every day until the larvae turn into pupae. Observe and record the state of the pupae once a day until the adults emerge. Single head / grain feeding, 8-10 heads / grain per batch, repeated 4 batches.

[0034]Calculate the development period of each egg, each larva or pupa, and the period of development from egg to adult.

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Abstract

The invention belongs to the field of insect breeding, and in particular relates to a method for raising South American tomato leafminer with a single head. The method for raising the South American tomato leaf miner with a single head of the present invention comprises the step of raising the South American tomato leaf miner from eggs to pupae in a transparent breeding box with fresh tomato leaves as host plants. When the present invention raises South American tomato leaf miner with a single head, it not only has a good feeding effect on eggs, but also has a better feeding effect on larvae and pupae; a cylindrical transparent plastic feeding box is adopted, and the material of the feeding box is completely transparent and can be directly observed , convenient and fast; the fresh-keeping time of the host leaves is long; therefore, the method of the present invention has strong practicability and can meet the needs of single insect breeding and subsequent related research.

Description

technical field [0001] The invention belongs to the field of insect breeding, and in particular relates to a method for raising South American tomato leafminer with a single head. Background technique [0002] South American tomato leaf miner Tuta absoluta (Meyrick), alias tomato leaf miner, tomato wheat miner, tomato wheat moth, English preferred common name the tomato leafminer, international common name the South American tomatomoth, belongs to Lepidoptera (Lepidoptera) wheat Moth family (Gelechiidae), native to Peru in the west of South America, is a worldwide quarantine invasive pest, which mainly harms tomatoes (including processed tomatoes, fresh tomatoes, cherry tomatoes / cherry tomatoes, etc.), eggplants, potatoes, etc. , mushrooms, ginseng fruit, tobacco, etc., and spread rapidly, the yield reduction rate is as high as 50-80% when it occurs seriously, and even destroys the seeds for replanting. At present, although the South American tomato leafminer only occurs in...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 张桂芬张毅波刘万学王玉生武强郭建洋万方浩
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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