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Related marker of oral squamous cell carcinoma metastatic potential and application of related marker

A technology for squamous cell carcinoma and oral cavity, which is applied in the field of biomedicine and can solve the problems of reduced quality of life and destruction of facial appearance.

Active Publication Date: 2019-11-08
SUINING CENT HOSPITAL
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, after comprehensive treatment such as surgery, radiotherapy and chemotherapy, the facial appearance of advanced patients is severely damaged, and the quality of life is significantly reduced.

Method used

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  • Related marker of oral squamous cell carcinoma metastatic potential and application of related marker
  • Related marker of oral squamous cell carcinoma metastatic potential and application of related marker
  • Related marker of oral squamous cell carcinoma metastatic potential and application of related marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1 Screening for Molecular Markers Related to the Malignancy of Oral Squamous Cell Carcinoma

[0048] 1. Clinical research object:

[0049] Selected 15 cases of oral squamous cell carcinoma tissue resected by the Department of Stomatology and Maxillofacial Surgery of the hospital (all patients had not undergone radiotherapy and chemotherapy before operation), including 6 cases of well-differentiated (Grade III, G3) patients and moderately differentiated (Grade II, G2) patients. ) patients in 4 cases, and poorly differentiated (grade I, G1) patients in 5 cases (diagnosed by two experienced pathologists to determine the pathological grade of all cancer tissues).

[0050] Inclusion criteria: (1) No chemotherapy or radiotherapy was performed before operation; (2) OSCC was confirmed by postoperative pathology.

[0051] Exclusion criteria: (1) patients with recurrence; (2) no concurrent neck dissection; (3) no pathological confirmation.

[0052] 2. Tissue RNA extract...

Embodiment 2

[0080] Example 2 QPCR verification of the relationship between candidate genes and the malignancy of oral squamous cell carcinoma

[0081] Based on the results of previous high-throughput sequencing and according to the size of the P value, we selected the SIX4 gene for verification.

[0082] 1. Research object:

[0083] Selected 90 cases of oral squamous cell carcinoma tissue resected by the Department of Stomatology and Maxillofacial Surgery of the hospital (all patients had not undergone radiotherapy and chemotherapy before operation), including 30 cases of well-differentiated (G3) patients, 30 cases of moderately differentiated (G2) patients, and 30 cases of low-differentiated (G2) patients. There were 30 differentiated (G1) patients (diagnosed by two experienced pathologists to determine the pathological grade of all cancer tissues). The inclusion and exclusion criteria are the same as in Example 1.

[0084] 2. RNA extraction and detection

[0085] Carry out according ...

Embodiment 3

[0098] Example 3 SIX4 gene affects the proliferation and migration of oral squamous cell carcinoma

[0099] 1. Cell culture

[0100] Oral squamous cell carcinoma HN6 cells were cultured with high-glucose DMEM complete medium (containing streptomycin-penicillin, 10% fetal bovine serum). The experiment was carried out using cells in the logarithmic growth phase, and the culture conditions were 37°C, 5% CO 2 ,>95% saturated humidity.

[0101] 2. Cell transfection

[0102] Using transfection reagent liposome and serum-free high-sugar DMEM solution containing antibiotics, the transfection system is 250 μl, and 5*10 4 HN6 cells were grown in 24-well plates at 37°C, 5% CO 2 Cultivate overnight in an incubator; place the diluted LipofectamineTM 2000 at room temperature for 5 minutes, mix with diluted siRNA and leave at room temperature for 20 minutes to form a transfection complex. Add the above-mentioned mixed transfection complex to the cultured cell liquid (set irrelevant siRN...

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Abstract

The invention discloses a SIX4 gene regarded as a molecule marker for assessing oral squamous cell carcinoma metastatic potential. Through high-throughput sequencing and QPCR research, the fact that the content of the SIX4 gene in well differentiated oral squamous cell carcinoma is lower than that in poorly differentiated oral squamous cell carcinoma is informed. According to the research result,the invention further provides a kit used for assessing the oral squamous cell carcinoma metastatic potential, and the kit has an obvious detection effect, and can be clinically used.

Description

technical field [0001] The present invention relates to the field of biomedicine. Specifically, the present invention relates to markers related to the malignancy of oral squamous cell carcinoma and applications thereof, more specifically, to the application of SIX4 as a molecular marker of malignancy of oral squamous cell carcinoma. Background technique [0002] Oral squamous cell carcinoma is one of the ten most common cancers in the world. It is the most common oral tumor, accounting for more than 90%. There are about 500,000 new cases every year, and it is still on the rise in recent years. Oral squamous cell carcinoma not only endangers the life of patients, but also affects their beauty and appearance, as well as the quality of life of patients such as chewing, speech, and swallowing. [0003] The occurrence of oral squamous cell carcinoma is related to many factors, and it is often the result of the combined effect of multiple factors. Oral cancer is defined in clini...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886C12N15/11G01N33/68G01N33/574
CPCC12Q1/6886C12Q2600/118C12Q2600/156G01N33/57407G01N33/68G01N2333/46
Inventor 杨森郭丽娟
Owner SUINING CENT HOSPITAL
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