Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of retrograde recombinant adeno-associated virus type 2 in infected spiral neurons

A neuron, virus technology, applied in recombinant adeno-associated virus and its application field

Active Publication Date: 2019-11-08
SHANGHAI TECH UNIV
View PDF3 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But how to improve the specificity of AAVs has not been solved

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of retrograde recombinant adeno-associated virus type 2 in infected spiral neurons
  • Application of retrograde recombinant adeno-associated virus type 2 in infected spiral neurons

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0017] Below in conjunction with specific embodiment, further illustrate the present invention. It should be understood that these examples are only used to illustrate the present invention and are not intended to limit the scope of the present invention. In addition, it should be understood that after reading the teachings of the present invention, those skilled in the art can make various changes or modifications to the present invention, and these equivalent forms also fall within the scope defined by the appended claims of the present application.

[0018] 1. Experimental materials and methods

[0019] A. Plasmid acquisition:

[0020] Both the AAV2 target plasmid and packaging plasmid were directly purchased from Addgene: pAAV-CAG-EGFP (Addgene, 28014); pAAV2-retro Helper (Addgene, 81070); pHelper (Addgene, 112867);

[0021] The difference between retrograde recombinant AAV2 and anterograde AAV2 is that the Cap cDNA sequence of one of the packaging plasmids (pAAV2-retroH...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides application of a retrograde recombinant adeno-associated virus type 2 in infected spiral neurons. The application is characterized in that the retrograde recombinant adeno-associated virus type 2 is injected into the cochlea by cochlear round window injection, and the retrograde recombinant adeno-associated virus type 2 is efficiently and specifically infects the spiral neurons. The invention provides a new usage of the recombinant adeno-associated virus type 2 in the infection of the cochlear spiral neurons, which has not been reported before, and the retrograde recombinant adeno-associated virus type 2 is capable of efficiently and specifically infecting the cochlear spiral neurons, and has good application prospects in the preparation of drugs for treating hereditary deafness and deafness related diseases, especially the drugs for auditory sensory neuron-spiral neuron related diseases.

Description

technical field [0001] The invention belongs to the field of recombinant adeno-associated virus and its application, and in particular relates to the application of a retrograde recombined type 2 adeno-associated virus in spiral neurons. Background technique [0002] Injecting genes that can be improved and corrected into our peripheral and sensory organs, spinal cord and brain to achieve long-term stable and effective therapeutic effects is the top priority of current genetic therapy. However, because there are physiological barrier systems between our organs or inside our organs, such as the most familiar blood-brain barrier (Blood-Brain Barriers, BBB); therefore, it is particularly important to find technical means that can efficiently penetrate the barrier system . The current common technical method is to carry out repeated drug injections into the brain tissue, retina and cochlear system. However, the disadvantages are high tissue damage and short drug efficacy perio...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N15/864C12N5/10A61K35/76A61P27/16
CPCC12N15/86C12N5/0619A61K35/76A61P27/16C12N2750/14143C12N2800/107C12N2510/00Y02A50/30
Inventor 陈鑫何水金
Owner SHANGHAI TECH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com