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Preparation and application of nano-immunological preparation based on porous calcium carbonate

A technology of porous calcium carbonate and immune preparations, which can be applied to medical preparations without active ingredients, medical preparations containing active ingredients, and active ingredients of aluminum/calcium/magnesium, etc., which can solve the failure of tumor immunotherapy and the limited effect of immune response. , the toxicity of immune adjuvants and other problems, to achieve the effect of eliminating small lesions, high immune activation efficiency, and good therapeutic effect

Active Publication Date: 2019-11-08
SHANDONG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Studies have shown that the commonly used immune vaccine method has the following problems: first, the immune response effect caused by the immune vaccine is limited, generally need to add immune adjuvant to strengthen the immune response, but the toxicity of immune adjuvant (such as aluminum hydroxide) is relatively large
And the immunosuppressive microenvironment in the tumor tissue is usually difficult to break, which can easily lead to the failure of tumor immunotherapy

Method used

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  • Preparation and application of nano-immunological preparation based on porous calcium carbonate
  • Preparation and application of nano-immunological preparation based on porous calcium carbonate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 is based on the preparation of porous calcium carbonate nano-preparation

[0031] (1) Synthesis of porous CaCO by gas diffusion reaction 3 Nanoparticles: 220 mg CaCl 2 ·H 2 O and 8 g NH 4 HCO 3 At the same time, put it in a vacuum drying room, control the temperature at 25 ° C, and keep the whole system in a vacuum environment for 24 hours, the obtained CaCO 3 Nanoparticles were separated by centrifugation at 12000 rpm. Then CaCO in absolute ethanol 3 IDOi is loaded in the channel to get CaCO 3 @IDOi (abbreviated as CaI).

[0032] (2) The above nanoparticles are modified with liposomes. First, 20 mg of CaI in ethanol and 2 mg of DOPA were mixed. Then DPPC, cholesterol and DSPE-PEG were added to the above solution at a ratio of 4:4:2M, the solvent was spin-dried by a rotary evaporator, and the obtained nanoparticles were dissolved in an aqueous solution for further use. Then, the obtained nanoparticles were stirred with 200 mg PEI to prepare CaCO ...

Embodiment 2

[0036] Embodiment 2 mouse modeling experiment

[0037] 2.1 In vivo test

[0038] Select female Balb / c mice as research objects, and make tumor-bearing mouse models by subcutaneously injecting mouse breast cancer cells until the tumor grows to 50mm 3 Start medication. The administration groups were CaIPC (experimental group), LIPC, and normal saline. The tumor-bearing mice were administered intravenously, and the tumor volume and weight were measured every other day during the experimental period. The experimental results show that the CaIPC-treated mice in Example 1 have the slowest tumor growth rate, indicating that the present invention can effectively inhibit tumor growth in the mouse model. On the contrary, the LIPC group showed not very excellent tumor suppressive effect compared with the normal saline group, and this result indicated that only the group treated in the present invention showed the optimal tumor therapeutic effect. Body weight is an important parameter...

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Abstract

The invention belongs to the field of pharmaceutical preparations, and particularly relates to a nano-immunological preparation based on porous calcium carbonate. Porous CaCO3 nanoparticles are used as carriers, pore passages of the porous CaCO3 nanoparticles are internally loaded with an immune blocking inhibitor IDOi, the porous CaCO3 nanoparticles are coated with a liposome layer, and the surface of the liposome layer carries immunoregulatory nucleic acid CpG ODNs. The nano-immunological preparation synthesized has good biocompatibility and high safety, and has good therapeutic and recurrence-preventing effects on mouse breast cancer, the preparation process is simple and easy to implement, and the nano-immunological preparation has good application prospects in the field of anti-tumordrug preparation.

Description

technical field [0001] The invention belongs to the field of pharmaceutical preparations, in particular to a nano-immune preparation made of porous CaCO 3 The nanoparticle is a carrier, and the inside of the hole is loaded with the immune blocking inhibitor IDOi, and the surface carries the immune regulation nucleic acid CpG ODNs, its synthesis method and its application in the field of strengthening the curative effect of breast cancer and preventing its recurrence. Background technique [0002] Cancer is a malignant tumor that seriously threatens human health. The recurrence of primary tumor after treatment is the main cause of death for most cancer patients. At present, the conventional clinical treatment methods for tumors are mainly surgery, radiotherapy and chemotherapy, but after using these treatment methods, there will still be residual cancer cells in the body (ie, small lesions), and these small lesions are the main reason for cancer recurrence. Therefore, it is ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/69A61K31/7105A61K45/00A61K33/10A61P35/00A61P37/04
CPCA61K31/7105A61K47/6915A61K47/6949A61K45/00A61P35/00A61P37/04A61K33/10A61K2300/00
Inventor 唐波李艳华李娜潘伟
Owner SHANDONG NORMAL UNIV
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