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Light/temperature double sensitive hydrogel and preparation method thereof

A hydrogel and low-temperature technology, applied in the field of medical materials, can solve problems such as weak mechanical properties and poor stability, and achieve the effects of improving mechanical strength, shortening gelation time, and good development and application potential

Inactive Publication Date: 2019-11-05
OCEAN UNIV OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, as a polymer with temperature-sensitive gel-forming properties, hydroxybutyl chitosan is limited by its poor stability and weak mechanical properties under physiological conditions.

Method used

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  • Light/temperature double sensitive hydrogel and preparation method thereof
  • Light/temperature double sensitive hydrogel and preparation method thereof
  • Light/temperature double sensitive hydrogel and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Prepare HBC with reference to patent CN201110214776.X;

[0024] (2) Accurately weigh 0.5 g of HBC, dissolve it in 25 mL of deionized water, adjust the pH of the solution to 9.0 with 1M NaOH; slowly add 0.141 mL of glycidyl methacrylate (GMA) dropwise at a molar ratio of HBC:GMA=1:0.5 , stirred and reacted for 24 hours at low temperature and protected from light, and then adjusted the pH of the solution to neutral with 1M HCl; dialyzed the solution for 48 hours and freeze-dried to obtain MHBC; dissolved MHBC in deionized water at 4°C to a final concentration of 3 %, warming up to 25°C to obtain MHBC hydrogel;

[0025] (3) Accurately weigh 30 mg of MHBC and dissolve it in 1 mL of deionized water containing 0.15 w / v photoinitiator Irgacure 2959, and irradiate the solution with ultraviolet light for 3 min to obtain photocrosslinked MHBC hydrogel.

Embodiment 2

[0027] (1) Prepare HBC with reference to patent CN201110214776.X;

[0028] (2) Accurately weigh 0.5 g of HBC, dissolve it in 25 mL of deionized water, adjust the pH of the solution to 9.0 with 1M NaOH; slowly add 0.212 mL of glycidyl methacrylate (GMA) dropwise at a molar ratio of HBC:GMA=1:0.75 , stirred and reacted for 36 hours at low temperature and protected from light, and then adjusted the pH of the solution to neutral with 1M HCl; dialyzed the solution for 48 hours and freeze-dried to obtain MHBC; dissolved MHBC in deionized water at 4°C to a final concentration of 3 %, warming up to 20°C to obtain MHBC hydrogel;

[0029] (3) Accurately weigh 30 mg of MHBC and dissolve in 1 mL of deionized water containing 0.20 w / v photoinitiator Irgacure 2959, and irradiate the solution with ultraviolet light for 2 min to obtain photocrosslinked MHBC hydrogel.

Embodiment 3

[0031] (1) Prepare HBC with reference to patent CN201110214776.X;

[0032] (2) Accurately weigh 0.5 g of HBC, dissolve it in 25 mL of deionized water, adjust the pH of the solution to 9.0 with 1M NaOH; slowly add 0.282 mL of glycidyl methacrylate (GMA) dropwise at a molar ratio of HBC:GMA=1:1 , stirred and reacted for 48 hours at low temperature and protected from light, and then adjusted the pH of the solution to neutral with 1M HCl; dialyzed the solution for 48 hours and freeze-dried to obtain MHBC; dissolved MHBC in deionized water at 4°C to a final concentration of 3 %, warming up to 18°C ​​to obtain MHBC hydrogel;

[0033] (3) Accurately weigh 30 mg of MHBC and dissolve it in 1 mL of deionized water containing 0.25 w / v photoinitiator Irgacure 2959, and irradiate the solution with ultraviolet light for 1 min to obtain photocrosslinked MHBC hydrogel.

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Abstract

The invention discloses a light / temperature double sensitive hydrogel and a preparation method thereof, wherein the temperature sensitive part of the hydrogel is obtained by introducing hydroxybutyl into a chitosan molecular chain (Patent No. 201110214776.X), and the free amino group of hydroxybutyl chitosan and the epoxy group of glycidyl methacrylate are subjected to a ring-opening reaction to introduce a photosensitive group so as to obtain the photosensitive part. According to the present invention, the methacrylylated hydroxybutyl chitosan hydrogel is in a solution state at a low temperature, can be subjected to flowing injection, and can form a hydrogel within 1 min when a temperature is raised to around 37 DEG C; a photoinitiator is added into a prepolymer solution, the obtained solution is exposed to ultraviolet light, and the photoinitiator generates free radicals to initiate methacryloyl-substituted chain polymerization, such that the strength of the gel is significantly enhanced; and the light / temperature double sensitive hydrogel has good application prospects in the fields of tissue engineering scaffolds or drug sustained-release delivery and the like.

Description

technical field [0001] The invention belongs to the technical field of medical materials, and relates to a preparation method of light / temperature dual-sensitive hydrogel. Background technique [0002] Photocrosslinkable hydrogels derived from natural polymers are often used in tissue engineering due to their excellent biocompatibility and ability to facilitate cell growth. Because the structure of the hydrogel is very similar to the natural extracellular matrix, it provides a suitable microenvironment for cells and tissues. It has been found that some photocrosslinkable and thermosensitive gel-forming polymers may have great potential for various biomedical applications, such as injectable delivery systems and cell culture. [0003] Chitosan is a naturally abundant polymer whose polysaccharide backbone is considered an attractive candidate for extracellular matrix replacement. It has proven biocompatibility and has been successfully used in a variety of applications rangi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08C08J3/075C08J3/24C08J3/28C08L5/08
CPCC08B37/003C08J3/075C08J3/24C08J3/28C08J2305/08
Inventor 孔明罗欣刘艺丹陈西广冯超程晓杰
Owner OCEAN UNIV OF CHINA
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