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Vaccinium bracteatum thunb seed polysaccharide extracting process and application thereof

An extraction process, the technology of Wufanzi, which is applied in the field of extraction and separation, to achieve the effect of improving extraction efficiency and accurate and reliable extraction process conditions

Inactive Publication Date: 2019-10-29
南京先华生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is no report on the research on the polysaccharides of black rice

Method used

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  • Vaccinium bracteatum thunb seed polysaccharide extracting process and application thereof
  • Vaccinium bracteatum thunb seed polysaccharide extracting process and application thereof
  • Vaccinium bracteatum thunb seed polysaccharide extracting process and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1: Extraction process optimization and component analysis of black rice polysaccharide

[0033] Step 1: Take the Wufanzi collected in mid-September, wash it with clean water, dry the surface water at room temperature, freeze-dry and crush it, and pass it through a 60-mesh sieve for later use;

[0034] Step 2: Add ethyl acetate with a liquid-to-solid ratio of 10:1 to the Wufanzi powder obtained in Step 1 to remove monosaccharide and pigment impurities.

[0035] Step 3: Add the extractant (distilled water) whose liquid-solid mL / g ratio is 15:1, 20:1, 25:1, 30:1, 35:1 to the Wufanzi powder obtained in Step 2; extraction temperature 35°C, 45°C, 55°C, 65°C, and 75°C, respectively; under the conditions of ultrasonic power of 60W, 120W, 180W, 240W, and 300W, ultrasonic extraction for 10min, 20min, 30min, 40min, and 50min, and then centrifuged, the The obtained centrifuge is concentrated by rotary evaporation and then freeze-dried to obtain the crude polysaccharide of ...

Embodiment 2

[0073] Embodiment 2: antibacterial effect of black rice polysaccharide

[0074] Step 1: Preparation of black rice polysaccharide

[0075] Take 1000g of dry powder of Wufanzi, use the optimized extraction process of Wufanzi polysaccharide obtained in Example 1 (ultrasonic power is 120W, ultrasonic extraction time is 41min, liquid-to-solid ratio is 25:1, ultrasonic extraction temperature is 64°C, carry out Preparation, separation and purification of black rice polysaccharide, polysaccharide was freeze-dried to obtain 100.1g light yellow polysaccharide, and the yield of black rice polysaccharide was 10.01%.

[0076] Step 2: Antibacterial effect of black rice polysaccharide

[0077] (1) Tested strains:

[0078] Escherichia coli, Staphylococcus aureus, Bacillus subtilis, Rhodotorula and Penicillium (the above strains were provided by Microbiology Laboratory of Suzhou University of Science and Technology).

[0079] (2) Medium:

[0080] Beef extract-peptone medium: 3g beef extrac...

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Abstract

The invention provides a vaccinium bracteatum thunb seed polysaccharide extracting process and application thereof. The vaccinium bracteatum thunb seed polysaccharide extracting process comprises thefollowing steps that collected fresh vaccinium bracteatum thunb seeds are taken, washed to be aired, subjected to freeze drying, smashed and then sieved; various monosaccharide and fat-soluble pigmentsubstances in vaccinium bracteatum thunb seed powder are removed through ethyl acetate; and by taking distilled water as an extraction agent, ultrasonic extraction, refrigerated centrifugation, rotary evaporation concentration, low-temperature settlement and impurity washing and freeze drying are conducted, and thus vaccinium bracteatum thunb seed polysaccharide is obtained. The extracting process of the vaccinium bracteatum thunb seed polysaccharide is optimized through single-factor experiment and Box-Behnken center combination response face test design, thus the optimal extracting processis screened, and the bacteriostatic action of the vaccinium bracteatum thunb seed polysaccharide is researched. The vaccinium bracteatum thunb seed polysaccharide extracting process and application thereof are researched firstly, a method is convenient to operate, and a target product is high in extraction rate and purity. The vaccinium bracteatum thunb seed polysaccharide has strong bacteriostatic and bactericidal activity to escherichia coli, staphylococcus aureus, bacillus subtilis, rhodotorula glutinis and penicillium and thus has wide application prospects.

Description

technical field [0001] The invention belongs to the field of extraction and separation, and relates to a polysaccharide extraction process and its application, in particular to an extraction method of black rice polysaccharide and the antibacterial and bacteriostatic application of the polysaccharide. Background technique [0002] Wufan, also known as Nanzhu, its scientific name in Latin Vaccinium bracteatum Thunb. Evergreen shrubs or small trees, 2-6 meters high; many branches, young branches pubescent or glabrous, old branches purple-brown, glabrous. Leaf blade thinly leathery, elliptic, diamond-elliptic, lanceolate-elliptic to lanceolate; petiole 2-8mm long, usually glabrous or pubescent. Racemes terminal and axillary, 4-10 cm long, with many flowers, rachis densely pubescent and sparsely glabrous; bracts leaflike, lanceolate, 0.5-2 cm long, both sides covered with fine hairs along the veins Or both sides are nearly glabrous, margins are serrated, persistent or shed, b...

Claims

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Application Information

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IPC IPC(8): C08B37/00A61P31/04A61P31/10
CPCA23V2002/00A23L33/125A61P31/04A61P31/10C08B37/0003A23V2250/50Y02A50/30
Inventor 王桃云邱业先扶教龙胡翠英陈宏伟钱玮韩承勇纪滨钟礼杰
Owner 南京先华生物科技有限公司
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