Candida utilis three-gene expression strain capable of degrading protein and building method of Candida utilis three-gene expression strain

Abstract

The invention relates to the field of genetic engineering and fermentation engineering and provides a Candida utilis three-gene expression strain capable of degrading protein. The Candida utilis three-gene expression strain is built by integrating aminopeptidase, carboxypeptidase and endo protease to the Candida utilis genome through a Candida utilis expression vector. Compared with the prior art,the Candida utilis three-gene expression strain has the advantages that when the Candida utilis three-gene expression strain is used to treat kitchen waste fermentation residual liquid, organic matter in the kitchen waste fermentation residual liquid can be further utilized, a large amount of protein in the kitchen waste fermentation residual liquid can be decomposed into amino acid, the residualprotein pollution problem can be solved, and the produced amino acid is the raw material of pharmacy, biological feed and organic fertilizer and has a certain economic value.

Description

technical field [0001] The invention relates to the fields of genetic engineering and fermentation engineering, and more specifically relates to a protein-degrading Candida utilis dual-gene expression strain and a construction method thereof. Background technique [0002] Kitchen waste, commonly known as swill, is solid waste formed by residents in their daily lives. The amount of food waste is increasing rapidly with the rapid increase of population and rapid economic development. Residual meat, vegetables, oil, and rice are The main substance in the garbage. In terms of chemical composition, it mainly contains organic components such as cellulose, protein, sugar, fat, etc. It has the characteristics of high water content, high proportion of organic matter, and high salt content. Due to the huge output of food waste, many toxic and harmful pathogenic microorganisms such as Staphylococcus aureus and Salmonella often breed in food waste rich in nutrients. If it is not proce...

AI Technical Summary

Problems solved by technology

There is an urgent need to find a practical and effective method for comprehensive utilization of kitchen waste. Unreasonable use of kitchen waste will not only cause serious waste of resources, but also cause serious environmental pollution

Incineration and use as feed are the traditional treatment methods for food waste; incineration not only has low utilization efficiency of food waste, but also emits a large amount of carbon dioxide, which aggravates global warming. A large amount of nitrogen, sulfur, and The production of compounds such as dioxin not only pollutes the air, but also causes great harm to the human body

After the food waste is processed, it is made into feed to feed livestock. The feed contains a large amount of toxic and harmful microorganisms and toxins that are difficult to degrade. Human health, and it contains a large amount of biological protein from different sources, and there is a potential biosafety risk in untreated feeding of livestock, so it is necessary to develop an effective method for comprehensive utilization of food waste, which can avoid Surrounding the city with garbage, protecting the environment, reducing harmful gases, and emitting greenhouse gases can also improve the utilization value of waste products and achieve comprehensive utilization of resources

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