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Method for measuring aspartame and alitame in food by reversed phase liquid chromatography mixed-standard sample-adding incremental process

A technology of reversed-phase liquid chromatography and aspartame, which is applied in the field of food testing, can solve the problems of different degrees of interference and side reactions in the measurement results, simultaneous and simultaneous measurement, and different background backgrounds, etc., achieving low cost, High accuracy and reduced interference effect

Pending Publication Date: 2019-10-18
UNIV FOR SCI & TECH ZHENGZHOU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method mainly has the following disadvantages: (1) it is necessary to prepare 5-7 standard solutions of different concentrations, which is time-consuming, laborious and costly; Simultaneous and synchronous measurement cannot be realized. When the instrument conditions and environmental factors (such as temperature, humidity, atmospheric pressure, voltage, operating factors, instrument performance, etc.) inevitably fluctuate, there will be certain differences, which will have different degrees of influence on the measurement results , introducing a certain error; (3) The background background of the standard liquid and the liquid to be tested are different, the difference is large, and the degree of interference to the measurement results or the degree of possible side reactions are different
This method of determination needs to use the standard solution of each component to determine the respective quantitative correction factors and relative correction factors, the preparation workload is large, and when it cannot be guaranteed that all components in the sample have peaks or the correction factors for unknown peaks are missing When the normalization method cannot be used for quantitative analysis, the application is limited

Method used

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  • Method for measuring aspartame and alitame in food by reversed phase liquid chromatography mixed-standard sample-adding incremental process
  • Method for measuring aspartame and alitame in food by reversed phase liquid chromatography mixed-standard sample-adding incremental process
  • Method for measuring aspartame and alitame in food by reversed phase liquid chromatography mixed-standard sample-adding incremental process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0080] Weigh about 5.000g (accurate to 0.001g) Coca-Cola carbonated beverage sample in a 50mL beaker, remove carbon dioxide on an ultrasonic oscillator, transfer all of it to a 25mL volumetric flask with water to make up the volume, mix well, and the measured components reach the concentration Balance, 4000r / min centrifuge for 5min, the concentration of aspartame and alitame in the supernatant and the lower layer are the same, centrifugation is to precipitate the particulate matter that may exist in the sample, take the supernatant and filter it with 0.45μm water system After membrane filtration, the liquid to be tested was obtained for chromatographic analysis.

[0081] Take two identical centrifuge tubes A and B, add 50 μL of 10 μg / mL aspartame and alitame mixed standard solution respectively, then add 100 μL of Coca-Cola test solution to centrifuge tube A, and dilute to 1 mL; Add 200 μL of Coca-Cola to-be-tested solution to centrifuge tube B, and dilute to 1 mL. Finally, t...

Embodiment 2

[0110] Weigh about 1.000g (accurate to 0.001g) chrysanthemum crystal sample in a 50mL beaker, add 10mL water, extract by ultrasonic vibration for 20min, transfer the extract into a 25mL volumetric flask, add 10mL water to the residue and ultrasonically extract for 10min, Transfer the extract into the same 25mL volumetric flask, dilute to volume with water, mix well, the measured components reach concentration balance, centrifuge at 4000r / min for 5min, aspartame and alitame in the supernatant and lower layer The concentrations are the same, centrifugation is to precipitate the particulate matter that may exist in the sample, take the supernatant and filter it with a 0.45 μm water filter membrane to obtain the test solution for chromatographic analysis.

[0111] Take two identical centrifuge tubes A and B, add 50 μL of 10 μg / mL aspartame and alitame mixed standard solution respectively, then add 100 μL of chrysanthemum crystal test solution to centrifuge tube A, and dilute to 1 m...

Embodiment 3

[0127] Weigh 5.000g (accurate to 0.001g) of yogurt sample into a 50mL centrifuge tube, add 10mL of ethanol to precipitate the protein in the yogurt sample, close the lid tightly, gently invert the centrifuge tube 5 times up and down (do not shake), and place After the centrifuge tube was mixed and vortexed for 10s, let it stand for 1min, centrifuged with a 4000r / min centrifuge for 5min, took the supernatant and filtered it into a 25mL volumetric flask, washed the filter residue with 8mL ethanol-water (2+1), and centrifuged the supernatant The solution was transferred into the same volumetric flask. Centrifugation is convenient for filtering a small amount of protein precipitation and other filter residues. After the components to be tested in the sample to be tested are completely extracted into the supernatant, use ethanol-water (2+1) to make up to 25mL, and use 0.45 After filtering with a μm organic filter membrane, the test solution was obtained for liquid chromatography ana...

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Abstract

According to a method disclosed by the invention, different volumes of to-be-tested liquids are added into two parts of completely identical aspartame and alitame mixed standard liquids; under the condition of the same liquid chromatography, mixed liquids are injected into a liquid chromatograph, a qualitative process is performed according to whether the change of component signals is added or not under a retention time condition, and a quantitative process is performed according to the peak output signal difference comparison after the mixed standard liquids are added with samples; and the method is high in efficiency, low in cost, few in interference and high in accuracy.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for determining aspartame and alitame in food by a reversed-phase liquid chromatography mixed standard adding sample addition method. Background technique [0002] Aspartame and Alitame are commonly added sweeteners in food, and their sweetness is 200 times and 2000 times that of sucrose respectively. Compound use can synergize, reduce costs, improve taste, and improve the stability of the sweetness of sweeteners. It not only has high sweetness, good taste quality, but also has a long storage period and no calories. However, if the intake is excessive, there will be adverse effects such as nervous system disorder and damage to the human liver, so it is one of the indicators often measured in food. [0003] At present, methods for the determination of aspartame and alitame in food include liquid chromatography (GB5009.263-2016), liquid chromatography-mass spectrometry, ion ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/04G01N30/14G01N2030/042G01N2030/146
Inventor 高向阳
Owner UNIV FOR SCI & TECH ZHENGZHOU
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