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Joint relieving method for stress damage of nanometer zinc oxide to plants by utilizing aspergillus aculeatus fungus and salicylic acid

A technology of nano-zinc oxide and Aspergillus aculeatus, which is applied in the field of plant-microbe joint restoration of nano-metal pollutants, can solve problems such as liver damage, increased embryo toxicity, and changes in soil bacterial communities, achieving low cost, good application prospects, easy-to-handle effects

Inactive Publication Date: 2019-10-18
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Exposure to ZnONPs causes liver damage in animals, alters soil bacterial communities, and increases embryotoxicity
However, there is no report on using the fungus Aspergillus aculeatus and salicylic acid to alleviate the stress damage of nano-zinc oxide to plants

Method used

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  • Joint relieving method for stress damage of nanometer zinc oxide to plants by utilizing aspergillus aculeatus fungus and salicylic acid
  • Joint relieving method for stress damage of nanometer zinc oxide to plants by utilizing aspergillus aculeatus fungus and salicylic acid
  • Joint relieving method for stress damage of nanometer zinc oxide to plants by utilizing aspergillus aculeatus fungus and salicylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method for alleviating the stress damage of nano-zinc oxide to plants by using the fungus Aspergillus aculeatus and salicylic acid, comprising the following steps: selecting healthy wild-type tobacco seedlings and transplanting them into the soil containing nano-zinc oxide, and the nano-zinc oxide in the soil The content of zinc oxide is 18mg / kg, add fungus Aspergillus aculeatus liquid and salicylic acid solution in the rhizosphere of plant, water, cultivate and grow, the concentration of fungus Aspergillus aculeatus liquid is 10 7 cfu / mL, add 15mL to each plant; the concentration of salicylic acid solution is 1.5mmol / L, add 100mL to each plant.

[0033] figure 1 The leaves were taken on the 15th day of planting, and the hydrogen peroxide content and chlorophyll content were measured on the 15th day of wild-type tobacco seedling growth, see figure 2 and image 3 . (WT+ZnONPs+SA+A.a in the figure is embodiment 1)

Embodiment 2

[0054] A method for alleviating the stress damage of nano-zinc oxide to plants by using the fungus Aspergillus aculeatus and salicylic acid, comprising the following steps: selecting healthy wild-type tobacco seedlings and transplanting them into the soil containing nano-zinc oxide, and the nano-zinc oxide in the soil The content of zinc oxide is 5 mg / kg, add fungus Aspergillus aculeatus liquid and salicylic acid solution in the rhizosphere of plant, cultivate and grow, the concentration of fungus Aspergillus aculeatus liquid is 106cfu / mL, every plant adds 20mL; The concentration of the salicylic acid solution was 2mmol / L, and 50mL was added to each plant.

Embodiment 3

[0056] A method for alleviating the stress damage of nano-zinc oxide to plants by using the fungus Aspergillus aculeatus and salicylic acid, comprising the following steps: selecting healthy wild-type tobacco seedlings and transplanting them into the soil containing nano-zinc oxide, and the nano-zinc oxide in the soil The content of zinc oxide is 30mg / kg, adds fungus Aspergillus aculeatus liquid and salicylic acid solution in the rhizosphere of plant, cultivates and grows, and the concentration of fungus Aspergillus aculeatus liquid is 10 8 cfu / mL, add 10mL to each plant; the concentration of salicylic acid solution is 1mmol / L, add 200mL to each plant.

[0057] After testing, in Example 2 and Example 3, the hydrogen peroxide content and chlorophyll content measured on the 15th day of wild-type tobacco seedling growth are similar to those in Example 1.

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Abstract

The invention discloses a joint relieving method for the stress damage of nanometer zinc oxide to plants by utilizing aspergillus aculeatus fungus and salicylic acid. The method comprises the steps that plant seedlings growing robustly are selected and transplanted into soil containing nanometer zinc oxide, aspergillus aculeatus fungus liquid and a salicylic acid solution are added to the rhizosphere of plants, and growth is achieved through culture. According to the method, the raw materials are easy to obtain, and the method is simple, easy to implement and low in cost and has a good application prospect. It is proved through an experiment that the aspergillus aculeatus fungus and the salicylic acid are utilized for jointly relieving the stress damage of the nanometer zinc oxide to the plants.

Description

technical field [0001] The invention relates to the field of plant-microorganism combined restoration of nanometer metal pollutants, in particular to a method for using the fungus Aspergillus aculeatus and salicylic acid to jointly alleviate the stress damage of nanometer zinc oxide to plants. Background technique [0002] Nanoparticles (NPs), a substance at least 100nm or smaller, have the function of single atom or molecule transfer and the transfer function of corresponding volume materials, which can greatly change the physical and chemical properties of substances, and may have positive or negative effects on living organisms, Therefore, it has received great attention. The rapid development of nanotechnology has led to the widespread use of nanoparticles in the size range of 1–100 nm in the cosmetic, food, industrial and agricultural industries. NPs will enter water, atmosphere, soil and other environments along with the transportation, storage, leakage, use and waste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G22/00A01G7/06A01G22/45
CPCA01G7/06A01G22/00A01G22/45
Inventor 关春峰张越王畅李倩
Owner TIANJIN UNIV
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