Reaction solution capable of improving specificity, primer pair, probe and kit for detecting canine parainfluenza

Abstract

The invention discloses a reaction solution capable of improving specificity of an inverse transcription nucleic acid chain type amplification reaction, primer pair, probe and kit for detecting canineparainfluenza. A method for improving the specificity of the inverse transcription nucleic acid chain type amplification reaction comprises the steps that in an inverse transcription reaction stage,recA protein and ATP are utilized for ensuring and improving the specificity of an inverse transcription product; compared with the prior art, the specificity of the inverse transcription product is significantly improved, and the non-specific amplification phenomenon does not occur on the inverse transcription product obtained by adopting the method in a later PCR reaction; the kit for detectingthe canine parainfluenza virus adopts primers and the probe which are modified by amino groups, the specificity is improved, the detection time is shortened, the diagnosis capacity is ensured to the greatest extent, and convenience is provided for clinical use.

Description

technical field [0001] The invention relates to the technical field of molecular biology, in particular to a reaction solution for detecting canine parainfluenza, a primer pair, a probe and a kit for improving the specificity and sensitivity of reverse transcription nucleic acid chain amplification reaction. Background technique [0002] Reverse transcription PCR (RT-PCR) is a widely used variant of the polymerase chain reaction (PCR). In RT-PCR, an RNA strand is reverse transcribed into complementary DNA, which is then used as a template for DNA amplification by PCR. Exponential amplification by RT-PCR is a very sensitive technique that can detect very low copy number RNA. RT-PCR is widely used in the detection of RNA viruses, the diagnosis of genetic diseases, and can be used to quantitatively monitor the content of a certain RNA. [0003] The key step of RT-PCR is the reverse transcription of RNA. There are two commonly used reverse transcriptases, namely avian myelobla...

AI Technical Summary

Problems solved by technology

[0007] The object of the present invention is to provide a kind of reaction solution, primer pair, probe and test kit for the detection of canine parainfluenza that improve the specificity of reverse transcription nucleic acid chain amplification reaction, thereby solve the poor specificity of reverse transcription reaction in the prior art , RT-PCR non-specific amplification is obvious; canine parainfluenza virus detection reagents - including the problem of poor specificity of nucleic acid detection reagents

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