Tilapia mossambica streptococcus agalactiae natural attenuated strain and attenuated live vaccine thereof

Abstract

The invention relates to an aquaculture animal vaccine, in particular to an attenuated live vaccine prepared from a tilapia mossambica streptococcus agalactiae natural attenuated strain TFJ0901. The streptococcus agalactiae natural attenuated strain TFJ0901 is preserved in China Center for Type Culture Collection of Wuhan University, China, and the preservation number is CCTCC NO: M 2016792. The ingredients of the attenuated live vaccine are live cells of the natural attenuated strain TFJ0901 and a phosphate buffer solution, and the cell concentration is 10<6>-10<8> CFU / ml. The vaccine has high safety and immunogenicity and can effectively prevent tilapia mossambica streptococcus agalactiae.

Description

technical field [0001] The invention relates to an aquaculture animal vaccine, in particular to a tilapia-sourced Streptococcus agalactiae (Streptococcus agalactiae) natural attenuated strain TFJ0901 strain and the attenuated live vaccine prepared therefrom. Background technique [0002] Streptococcus agalactiae is an important pathogenic bacterium that affects humans, animals and fish, and can cause neonatal meningitis in humans, mastitis in bovines, meningitis in fish, and sepsis. China is the largest country in the tilapia farming industry in the world, accounting for more than 50% of the world's entire tilapia industry. Before 2008, the outbreak of streptococcal disease in tilapia cultured in China was mainly caused by Streptococcus dolphin, while the outbreak of streptococcal disease in 2009-2011 was mainly caused by Streptococcus agalactiae, with a cumulative death rate of up to 30 -80%, the economic loss caused in 2011 alone was as high as 400 million U.S. dollars. Ac...

AI Technical Summary

Problems solved by technology

Before 2008, the outbreak of streptococcal disease in tilapia cultured in China was mainly caused by Streptococcus dolphin, while the outbreak of streptococcal disease in 2009-2011 was mainly caused by Streptococcus agalactiae, with a cumulative death rate of up to 30 -80%, the economic loss caused in 2011 alone was as high as 400 million U.S. dollars. According to epidemiological investigations in recent years, streptococcal disease outbreaks in tilapia in my country are still caused by Streptococcus agalactiae, and the annual cost to my country Big losses for tilapia industry

But at present, there is no data report on the natural attenuated live vaccine of tilapia Streptococcus agalactiae at home and abroad

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