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Intestinal microbial flora for evaluation of inflammatory state and prognosis of rheumatoid arthritis

A technology for assessing intestinal microbes and status, applied in the field of microbiology, can solve problems such as the evaluation of the condition and prognosis of rheumatoid arthritis that have not yet been seen in intestinal butyric acid-producing bacteria and non-fermenting bacteria

Active Publication Date: 2019-09-27
BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there is no relevant report on the combination of intestinal butyrate-producing bacteria and non-fermenting bacteria in the evaluation of the condition and prognosis of rheumatoid arthritis

Method used

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  • Intestinal microbial flora for evaluation of inflammatory state and prognosis of rheumatoid arthritis
  • Intestinal microbial flora for evaluation of inflammatory state and prognosis of rheumatoid arthritis
  • Intestinal microbial flora for evaluation of inflammatory state and prognosis of rheumatoid arthritis

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1 Enrichment of butyrate-producing bacteria in the feces of rheumatoid arthritis patients with negative anti-CCP antibodies, and enrichment of non-fermenting bacteria in the feces of patients with positive anti-CCP antibodies

[0067] Anti-cyclic citrullinated peptide antibody is an autoantibody with cyclic citrullinated polypeptide (CCP) as its antigen. It has high sensitivity and specificity to rheumatoid arthritis (RA), and is a highly specific marker for early diagnosis of RA. index. In this example, Illumina hiseq 2000 high-throughput sequencing technology was used to perform metagenomic sequencing on the stool samples of 29 cases of healthy people and 40 cases of initial untreated RA patients (15 cases of CCP antibody negative and 25 cases of CCP antibody positive). FastQC (Version0.11.5), Trimmomatic (Version 0.33, option:SLIDINGWINDOW:4:20MINLEN:40), FASTX-Toolkit (Version 0.0.13, option:-q 20-p 80) complete low-quality data filtering, using BWA For hum...

Embodiment 2

[0068] Example 2 The abundance ratio of butyric acid-producing bacteria / non-fermenting bacteria in the feces of patients with mild rheumatoid arthritis was significantly increased

[0069] According to literature reports and genome information, the present invention detects 12 genera Anaerostipes, Acetobacterium, Clostridium, Fusobacterium, Megasphaera, Treponema, Brachyspira, Filifactor, Butyrivibrio, Peptoniphilus, Anaerotignum with the ability to produce butyric acid from the samples collected in Example 1 , Roseburia, and 33 genera of non-fermenting bacteria that cannot directly utilize sugar but can utilize butyric acid Achromobacter, Burkholderia, Pseudomonas, Ralstonia, Robiginitalea, Stenotrophomonas, Tenacibaculum, Ornithobacterium, Cupriavidus, Delftia, Sphingomonas, Variovorax, Alcaligenes, Gramella, Zobellia, Curvibacter, Gilvibacter, Limnohabitans, Alicycliphilus, Bordetella, Roseomonas, Polaribacter, Paraburkholderia, Rhodoferax, Hydrogenophaga, Flavobacterium, Ca...

Embodiment 3

[0070] Example 3 Correlation between intestinal butyric acid-producing bacteria and non-fermenting bacteria and clinical indicators of rheumatoid arthritis

[0071] In this embodiment, the abundance of each genera of butyric acid-producing bacteria and non-fermenting bacteria mentioned and the erythrocyte sedimentation rate (ESR), C-reactive protein (CRP), DAS28.ESR, DAS28.CRP, rheumatoid factor (RF) of RA patients ) and anti-CCP antibody (Anti-CCP) clinical indicators, it was found that there was a strong correlation between the number of butyrate-producing bacteria and CCP indicators in RA patients ( Figure 2A, Table 2), 11 butyric acid-producing bacteria were negatively correlated with clinical indicators indicating disease severity such as rheumatoid factor (RF) and anti-cyclic citrullinated peptide antibody (anti-CCP), while non-fermentative bacteria were inversely correlated . The present invention uses the ratio (ratio) of the total abundance of butyric acid-producing...

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Abstract

The invention provides an intestinal microbial flora for evaluation of inflammatory state and prognosis of rheumatoid arthritis. The intestinal microbial flora comprises two types of intestinal bacteria such as intestinal butyric acid generating bacteria and intestinal non-fermentative bacteria, wherein the intestinal butyric acid generating bacteria comprise 12 microorganisms generating butyric acid bacterial genuses, and the intestinal non-fermentative bacteria comprise 33 non-fermentative bacterial genus microorganisms. The invention firstly proposes that butyric acid generating bacteria and non-fermentative bacteria in the intestinal flora can affect the inflammatory state and prognosis of RA by generating / consuming intestinal butyric acid and affecting the net production of intestinal butyric acid. Large samples are verified, and results show that the inflammatory state and prognosis of RA can be evaluated by obtaining the ratio of total abundance of butyric acid to non-fermentative bacteria through fecal metagenome detection, and the sensitivity and specificity are relatively high.

Description

technical field [0001] The present invention relates to the field of microbiome, in particular, relates to the intestinal microbial flora used for the evaluation of the inflammatory state and prognosis evaluation of rheumatoid arthritis. Background technique [0002] Rheumatoid arthritis (RA) is a chronic, inflammatory synovitis-based autoimmune disease of unknown etiology. It is characterized by polyarticular, symmetrical, and aggressive arthritis of the small joints of the hands and feet, often accompanied by extra-articular organ involvement and positive serum rheumatoid factor, which can lead to joint deformities and loss of function. The prevalence rate is 0.33%. There are about 5 million patients in my country, and most of them are young and middle-aged women. The disability rate reaches 75% without formal treatment. Commonly used steroidal anti-inflammatory drugs, joint injection of glucocorticoids and other treatments. The current treatment method can only control ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/689C12Q1/06C12R1/145C12R1/025C12R1/38C12R1/05C12R1/20C12R1/065
CPCC12N1/20C12Q1/689
Inventor 楚亚男康禹孟庆仁于军石兴邵长君王建陈婧
Owner BEIJING INST OF GENOMICS CHINESE ACAD OF SCI CHINA NAT CENT FOR BIOINFORMATION
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