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Clonorchis sinensis recombinant protein C<s>H<sc>B and application thereof in enteritis therapeutic drug

A clonorchis sinensis and recombinant protein technology, applied to the clonorchis sinensis recombinant protein CsHscB and its application in enteritis therapeutic drugs, can solve problems such as unclear mechanism of action, and achieve high application prospects and value, and significant treatment. Effect, the effect of improving weight change

Active Publication Date: 2019-09-24
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the Clonorchis sinensis recombinant protein CsHscB, as a member of the Hsp family, plays an important role in the immune regulation of Clonorchis sinensis infection and the development of pathological damage.

Method used

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  • Clonorchis sinensis recombinant protein C&lt;s&gt;H&lt;sc&gt;B and application thereof in enteritis therapeutic drug
  • Clonorchis sinensis recombinant protein C&lt;s&gt;H&lt;sc&gt;B and application thereof in enteritis therapeutic drug
  • Clonorchis sinensis recombinant protein C&lt;s&gt;H&lt;sc&gt;B and application thereof in enteritis therapeutic drug

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Embodiment 1, the preparation of clonorchis sinensis recombinant protein HscB

[0039] 1. Construction of recombinant expression vector

[0040] Primer design

[0041]Referring to the gene sequence of Clonorchis sinensis Henan strain (GenBank: DF143487.1), specific primers were designed using Primer premier 5 software to amplify the entire sequence of the Clonorchis sinensis HscB protein coding gene.

[0042] The restriction endonuclease BamHI cutting site (GGATCC) was introduced into the upstream primer P1. The sequence of the upstream primer P1 is as follows:

[0043] 5'-CAGCAAATGGGTCGCGGATCCATGTCATTTCGTCTCGCACCA-3'.

[0044] A restriction endonuclease XhoI cutting site (CTCGAG) was introduced into the downstream primer P2. The sequence of the downstream primer P2 is as follows:

[0045] 5'-GTGGTGGTGGTGGTGCTCGAGTTAATGCGGAGGAATATCAACTGT-3'.

[0046] The above primers were synthesized by Shanghai Biological Biotechnology Co., Ltd.

[0047] The extraction of the tot...

Embodiment 2

[0166] Example 2: Recombinant protein CsHscB improves DSS-induced ulcerative colitis

[0167] 1. Establishment and grouping of animal models of ulcerative colitis

[0168] Experimental animals: C57BL / 6 mice used in the experiment were purchased from Beijing Weitong Lihua Company, male, 6-8 weeks old, SPF grade, and were raised in the SPF grade animal room of Xuzhou Medical University after purchase. Food and light, and ensure proper temperature and humidity.

[0169] Grouping of experimental mice

[0170] Before establishing the model mice, the included experimental mice should be grouped first, and 32 mice were selected and divided into normal saline group (ie, NC group), rCsHscB group, DSS group and (DSS+ rCsHscB group) according to the principle of random distribution. ) group, with 8 mice in each group. (Note that rCsHscB is the abbreviation of recombinant protein CsHscB, and rCsHscB mentioned in the full text refers to recombinant protein CsHscB).

[0171] A Modeling ...

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Abstract

The invention relates to the field of biotechnology, in particular to a clonorchis sinensis recombinant protein C<s>H<sc>B and application thereof in enteritis therapeutic drug. The C<s>H<sc>B protein has an amino acid sequence shown in SEQ NO:2. According to the clonorchis sinensis recombinant protein C<s>H<sc>B and the application thereof in the enteritis therapeutic drug, through widespread and in-depth study, it is discovered that the recombinant protein C<s>H<sc>B has an obvious therapeutic effect on enteritis especially ulcerative colitis, and conditions such as body weight change, disease activity score, length of colon and inflammatory cell infiltration of animals suffering from colonitis can be obviously improved through therapy of the recombinant protein C<s>H<sc>B; and meanwhile, the clonorchis sinensis recombinant protein C<s>H<sc>B can obviously reduce the level of ulcerative colitis inflammatory factor (such as IL-6). The enteritis therapeutic drug containing the recombinant protein C<s>H<sc>B has an extremely high application prospect and value especially in ulcerative colitis field.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the clonorchis sinensis recombinant protein CsHscB and its application in enteritis treatment drugs. Background technique [0002] Ulcerative colitis (UC) is a non-specific intestinal disease related to autoimmunity and a type of inflammatory bowel disease (IBD). The lesions are continuously and diffusely distributed, mainly involving the rectum and sigmoid colon, and mainly invade the intestinal mucosa and submucosa, and can form erosions and ulcers. The clinical symptoms include diarrhea, abdominal pain, gastrointestinal bleeding and weight loss. The course of the disease is protracted, ranging from severity to recurrence. If the disease is not actively treated, it is very prone to canceration. [0003] Ulcerative colitis is a nonspecific inflammatory disease of the colon and rectum of unknown etiology. The infiltration and activation of inflammatory cells play an imp...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/17A61K47/64A61P1/04
CPCA61K38/00A61P1/04C07K14/43559C07K2319/21
Inventor 颜超郑葵阳汤仁仙张雨钊范春阳李向阳华慧董昕于倩张波
Owner XUZHOU MEDICAL UNIV
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