System for culturing oleaginous micro-algae by combining light guide pipe and raceway pond
A technology for oil-producing microalgae and runway ponds, which is applied in microorganism-based methods, bioreactors/fermenters for specific purposes, microorganisms, etc. and other problems, to achieve the effect of uniform light distribution, promotion of growth and reproduction, and high light utilization rate
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Embodiment 1
[0045] The present invention finds that when Chlorella grows under natural conditions, the solar effective radiation at the light saturation point of photosynthesis is 500 μmol·m -2 ·s -1 , the corresponding light illuminance is 27777.8lx.
[0046] According to the light attenuation curve experiment, it is found that the incident light of 2 000lx can meet the growth requirements of Chlorella with a depth of 500mm.
[0047] Therefore, the light illuminance at the distance of 500mm from the bottom of the algae liquid should be above 2000lx, and the light illuminance on the liquid surface should be as close as possible to 2 7777.8lx.
Embodiment 2
[0049] In actual situations, light will be refracted when it enters the algae liquid from the air, causing the irradiation range of the light pipe to exit the algae liquid to change when it enters the algae liquid. Therefore, it is necessary to measure the refractive index of the algae liquid and calculate The irradiation range is corrected. The refractive index (n) of the chlorella algae liquid and domestic sewage were measured respectively, and the experimental data and results are shown in Table 2-1.
[0050] Table 1 domestic sewage and chlorella algae liquid refractive index measurement results (see Figure 11 shown)
[0051]
[0052] For the same incident angle of light, the larger the refractive index, the smaller the exit angle (the angle between the exit light and the normal), and the smaller the illuminated area of the light guide in the water. In order to ensure the normal growth of Chlorella under harsh conditions, the minimum illuminated area should be consi...
Embodiment 3
[0054] In order to make the designed lighting scheme meet the lighting requirements in the whole culture period, the change of algae liquid turbidity in the culture cycle was studied, and the change of algae liquid turbidity in a culture cycle was sorted into a graph, as shown in figure 2 shown.
[0055] Depend on figure 2 It can be seen that at the initial stage of cultivation, the turbidity of the algae liquid of Chlorella is about 190NTU. Therefore, when designing the lighting scheme, the turbidity of the algae liquid is 190NTU as a reference value.
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