A kind of detection reagent and detection method of anthraquinone compounds

Abstract

The embodiment of the present invention provides a detection reagent and detection method for anthraquinone compounds. The detection reagent includes a hydrogen bond acceptor and a hydrogen bond donor corresponding to the hydrogen bond acceptor, the hydrogen bond acceptor includes L-menthol, and the hydrogen bond donor includes acetic acid. The steps of the detection method include heating the liquid to be tested, adding detection reagents to the liquid to be tested, mixing and then ice bathing to obtain a solidified layer and a liquid layer, pouring out the liquid layer, and adding a decoagulant to the solidified layer to decompose. After solidification, the content of anthraquinone compounds was determined by HPLC. The detection reagents and detection methods provided by the embodiments of the present invention reduce the cumbersome centrifugation and filtration. On the basis of the advantages of retaining ions, they also have the characteristics of low cost, environmental protection, and high efficiency. In addition, the application of DESs also avoids the use of demulsifiers. The use solves the problem of high cost of detecting anthraquinone compounds by mass spectrometry in the prior art.

Description

technical field [0001] The invention belongs to the technical field of quinone compound detection, in particular to a detection reagent and a detection method for anthraquinone compounds. Background technique [0002] Anthraquinones are widely distributed in Polygonaceae, Leguminosae, Rhamnosaceae, Rubiaceae and Liliaceae plants, and have various pharmacological effects such as laxative, antibacterial and anti-inflammatory, anticancer and antitumor. However, since anthraquinones such as emodin, rhein, and emodin methyl ether can inhibit the proliferation of HK-2 cells, the leakage rate of lactate dehydrogenase in cells increases, cells vacuolize, and mitochondrial membrane potential decreases. Long-term use of anthraquinones will produce reversible liver and kidney toxicity, especially kidney toxicity. Long-term use of substances containing anthraquinones will also damage the intestinal mucosal barrier, promote the release of pro-inflammatory factors such as tumor necrosis ...

AI Technical Summary

Problems solved by technology

[0005] One of the embodiments of the present invention provides a detection reagent for anthraquinone compounds, the detection reagent includes a hydrogen bond acceptor and a hydrogen bond donor corresponding to the hydrogen bond acceptor, and the hydrogen bond acceptor includes L - Menthol, the hydrogen bond donor includes acetic acid, which solves the problem of high cost of mass spectrometry detection of anthraquinone substances in the prior art

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