Method for preparing chitin by hydrolyzing shrimp and crab shells by utilizing alkaline protease

Abstract

The invention discloses a method for preparing chitin by hydrolyzing shrimp and crab shells by utilizing alkaline protease. The method comprises the steps: adding water into the shrimp and crab shells, and carrying out heating to cook the shrimp and crab shells; reducing the temperature to the normal temperature, then, transferring the shrimp and crab shells into a fermentation vessel, adding the alkaline protease into the fermentation vessel to carry out fermentation for 12-16 days to obtain a fermented mixture, wherein the mass of the alkaline protease is 0.3-0.4% of that of the cooked shrimp and crab shells; and carrying out solid-liquid separation on the fermented mixture to respectively obtain a fermentation liquid and a sediment. Complex enzyme method decomposition is adopted during shrimps and crabs are dissolved and decomposed in the method, so that the damage of an acidolysis decomposition method in a traditional process to the biological potency of proteins is avoided; and three high-purity products including chitin, protein powder and fat can be sequentially separated and extracted by one fermentation, so that the defect that only single product can be extracted in the traditional process is overcome, the comprehensive utilization ratio of raw materials is greatly increased, and the environment is not polluted in a production process.

Description

technical field [0001] The invention relates to the technical field of chitin production, in particular to a method for preparing chitin by hydrolyzing shrimp and crab shells with alkaline protease. Background technique [0002] Chitin (C8H13O5N)n, also known as chitin, chitin, the English name Chitin. It was discovered by French scholar Braconno in 1811 and extracted from crustacean shells by Ogier in 1823. Chitin has a wide range of applications, and can be used in industries such as fabrics, clothing, dyes, paper and water treatment. In agriculture, it can be used as insecticide and plant antiviral agent. In fishery, it is used as fish feed. Cosmetic beauty agent, hair protection, moisturizing agent, etc. Medical supplies can be used as contact lenses, artificial skin, sutures, artificial dialysis membranes and artificial blood vessels. Arthropods are mainly crustaceans, such as shrimps and crabs, which contain up to 58%-85% of chitin. [0003] In the prior art, the...

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Problems solved by technology

[0003] In the prior art, the methods for extracting chitin from shrimps and crabs are mostly acidolysis and decomposition methods, but only one substance of chitin in shrimps and crabs can be extracted by the acidolysis decomposition method, and the comprehensive utilization rate of raw materials is low. More waste is generated during the production process