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A method of detoxifying citrus by using microwave

A citrus and microwave technology, applied in the field of microwave applications, can solve the problems of slow heating rate, inability to heat the roots, unevenness, etc., and achieve the effects of temperature control, shortening the detoxification cycle, and preventing spread and spread

Active Publication Date: 2021-04-02
GUANGXI ACAD OF SCI +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microwave sterilization and insecticide have been well applied in the prevention and control of some diseases, but there is no microwave method to kill Phloem bacilli for citrus Huanglongbing
[0004] After Huanglongbing infects plants, the bacteria will form lesion spots on the phloem, which can infect almost all parts of citrus cultivars in each growth period, such as leaves, fruits, flowers, roots, seed coats and stems. To kill Phloem bacillus, all parts of the citrus should be heated evenly. Common heating methods such as hot air and hot steam have slow, uneven and inability to heat the roots, which have obvious defects.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A method utilizing microwaves to detoxify citrus, comprising the following steps:

[0033] (1) Detection of citrus Huanglongbing: Sampling citrus seedlings to be detoxified, qualitatively and quantitatively detecting Phloem bacillus, the pathogen of Huanglongbing in citrus;

[0034] (2) Preparation before detoxification: control the water content of the soil to 15%, place the citrus to be detoxified in a microwave device, and adjust and control the distance between each part of the citrus to be detoxified and the microwave transmitting antenna to 40-60 cm;

[0035] (3) Microwave pretreatment: Turn on microwave irradiation to pretreat citrus. The microwave irradiation frequency is 2.45 GHz, the power is 450 W, the microwave irradiation time is 10 s, and then pause for 15 s. Repeat the operation 8 times to heat the temperature of each part of the citrus. to 45°C and maintain a constant temperature for 60 s; the microwave irradiation is planar microwave irradiation, the plan...

Embodiment 2

[0041] A method utilizing microwaves to detoxify citrus, comprising the following steps:

[0042] (1) Detection of citrus Huanglongbing: Sampling citrus fruit trees to be detoxified, qualitatively and quantitatively detecting Phloem bacillus, the pathogen of Huanglongbing in citrus;

[0043] (2) Preparation before detoxification: control the water content of the soil to 20%, place the citrus to be detoxified in a microwave device, and adjust and control the distance between each part of the citrus to be detoxified and the microwave transmitting antenna to 20-40 cm;

[0044] (3) Microwave pretreatment: Turn on microwave irradiation to pretreat citrus. The microwave irradiation frequency is 2.45 GHz, the power is 800 W, the microwave irradiation time is 5 s, and then pause for 15 s. Repeat the operation 6 times to heat the temperature of each part of the citrus. to 44°C and maintain a constant temperature for 40 s; the microwave irradiation is planar microwave irradiation, the p...

Embodiment 3

[0050] A method utilizing microwaves to detoxify citrus, comprising the following steps:

[0051] (1) Detection of citrus Huanglongbing: Sampling citrus rootstocks to be detoxified, qualitatively and quantitatively detecting Phloem bacillus, the pathogen of Huanglongbing in citrus;

[0052] (2) Preparation before detoxification: control the water content of the soil to 5%, place the citrus to be detoxified in a microwave device, and adjust and control the distance between each part of the citrus to be detoxified and the microwave transmitting antenna to 80-100 cm;

[0053] (3) Microwave pretreatment: Turn on microwave irradiation to pretreat citrus. The microwave irradiation frequency is 2.45 GHz, the power is 100 W, the microwave irradiation time is 20 s, and then pause for 30 s. Repeat the operation 3 times to heat the temperature of each part of the citrus. to 40°C, and maintain a constant temperature for 30 s; the microwave irradiation is planar microwave irradiation, and ...

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Abstract

The invention discloses a method for detoxification of citrus by utilizing microwaves, and belongs to the technical field of microwave application. The method is characterized in that during microwave pretreatment, the microwave irradiation frequency is 1.0 GHz-3.0 GHz, the power is 100-900 W, the microwave irradiation time is 5-20 seconds, then pausing is carried out for 10-30 seconds, repetitive operation is carried out 3-10 times, the temperature of each part of the citrus is increased to 40-45 DEG C, and the constant temperature is maintained for 30-60 seconds; then it is adjusted that the microwave irradiation frequency is 350 MHz-950 MHz, the power is 1,000-3,000 W, the microwave irradiation time is 3-10 seconds, then pausing is carried out for 20-60 seconds, repetitive operation is carried out 5-20 times, the temperature of each part of the citrus is increased to 50-55 DEG C, the constant temperature is maintained for 1-5 minutes, and citrus detoxification is carried out by utilizing the microwaves. The method for detoxification of the citrus by utilizing the microwaves can make each part of the citrus reach the fatal temperature of candidatus liberibacter more quickly and evenly, and a tree body is not affected; under the non-thermal effect of a microwave electromagnetic field, nucleic acid, protein and other molecules in the candidatus liberibacter are easy to denature and inactivate to achieve double sterilization effects; the detoxificated citrus through the microwaves are detected by adopting GB / T 28062-2011 <Real-Time Fluorescence PCR Detection Method of Candidatus Liberibacter>, and the negative conversion ratio of the candidatus liberibacter is greater than or equal to 82%. The method is an efficient, quick and environment-friendly citrus detoxification method.

Description

technical field [0001] The invention belongs to the technical field of microwave applications, and in particular relates to a method for detoxifying citrus by using microwaves. Background technique [0002] Citrus Huanglongbing (Huanglongbing) is currently the number one devastating disease of citrus production in the world, which is caused by Phloem bacteria parasitic in the phloem of plants. Due to the serious harm and high fatality rate, and the lack of effective prevention and control methods so far, Huanglongbing is called the "cancer" of citrus. Therefore, researchers are committed to detoxification research on diseased citrus trees, that is, directly killing phloem bacteria in diseased trees through physical and chemical means. The use of heat treatment to prevent and control citrus diseases has a long history. It is mainly used for grafting and detoxification. In recent years, it has been applied to field control. Chinese patent CN201610566664 (application number) ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G13/00A01G7/06
CPCA01G7/06A01G13/00
Inventor 陈先锐黄志民黄端权谢能中
Owner GUANGXI ACAD OF SCI
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