Method for detoxification of citrus by utilizing microwaves

Abstract

The invention discloses a method for detoxification of citrus by utilizing microwaves, and belongs to the technical field of microwave application. The method is characterized in that during microwave pretreatment, the microwave irradiation frequency is 1.0 GHz-3.0 GHz, the power is 100-900 W, the microwave irradiation time is 5-20 seconds, then pausing is carried out for 10-30 seconds, repetitive operation is carried out 3-10 times, the temperature of each part of the citrus is increased to 40-45 DEG C, and the constant temperature is maintained for 30-60 seconds; then it is adjusted that the microwave irradiation frequency is 350 MHz-950 MHz, the power is 1,000-3,000 W, the microwave irradiation time is 3-10 seconds, then pausing is carried out for 20-60 seconds, repetitive operation is carried out 5-20 times, the temperature of each part of the citrus is increased to 50-55 DEG C, the constant temperature is maintained for 1-5 minutes, and citrus detoxification is carried out by utilizing the microwaves. The method for detoxification of the citrus by utilizing the microwaves can make each part of the citrus reach the fatal temperature of candidatus liberibacter more quickly and evenly, and a tree body is not affected; under the non-thermal effect of a microwave electromagnetic field, nucleic acid, protein and other molecules in the candidatus liberibacter are easy to denature and inactivate to achieve double sterilization effects; the detoxificated citrus through the microwaves are detected by adopting GB / T 28062-2011 <Real-Time Fluorescence PCR Detection Method of Candidatus Liberibacter>, and the negative conversion ratio of the candidatus liberibacter is greater than or equal to 82%. The method is an efficient, quick and environment-friendly citrus detoxification method.

Description

Technical field [0001] The invention belongs to the technical field of microwave application, and specifically relates to a method for detoxifying citrus by using microwave. Background technique [0002] Citrus Huanglongbing (Huanglongbing) is currently the number one devastating disease in global citrus production. It is caused by the phloem bacteria parasitizing the phloem of plants. Due to the serious harm and high fatality rate, and the lack of effective prevention and control methods so far, Huanglongbing is called citrus "cancer". Young trees infected with Huanglong disease generally die within 2-3 years, and adult trees die within 5-8 years. In addition to citrus, Rutaceae plants such as orange, pomelo, yellow peel, mulberry and wolfberry are also affected by citrus yellow dragon disease. For fruit trees infected with Huanglong disease, the most common method is to dig out the whole tree and destroy them in large quantities, but this method will cause great economic loss...

AI Technical Summary

Problems solved by technology

Microwave sterilization and insecticide have been well applied in the prevention and control of some diseases, but there is no microwave method to kill Phloem bacilli for citrus Huanglongbing

[0004] After Huanglongbing infects plants, the bacteria will form lesion spots on the phloem, which can infect almost all parts of citrus cultivars in each growth period, such as leaves, fruits, flowers, roots, seed coats and stems. To kill Phloem bacillus, all parts of the citrus should be heated evenly. Common heating methods such as hot air and hot steam have slow, uneven and inability to heat the roots, which have obvious defects.