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Detection method for identifying adulterant in buffalo milk

A detection method and technology for buffalo milk are applied in the detection field of identifying adulteration in buffalo milk, which can solve the problems of large milk production, expensive equipment, and inability to match common basic laboratories, and achieves strong specificity and reproducibility. Good, reproducible and specific results

Pending Publication Date: 2019-09-10
GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above methods all use casein in buffalo milk as the research object to identify the source of buffalo, and the equipment used is expensive, which cannot be matched by ordinary basic laboratories.
However, the number of Holstein

Method used

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  • Detection method for identifying adulterant in buffalo milk
  • Detection method for identifying adulterant in buffalo milk
  • Detection method for identifying adulterant in buffalo milk

Examples

Experimental program
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Example Embodiment

[0025] Example 1

[0026] A detection method for identifying adulteration in buffalo milk includes the following steps:

[0027] (1) Sample collection and instrument preparation: Collect buffalo milk and milk samples to be tested and store them in refrigeration. At the same time, prepare the HPLC automatic sampling chromatograph and its matching chromatographic columns and clean them for later use; the milk to be tested is mixed with Holstein milk The temperature of cold storage is 4°C, and the chromatographic column is a ZORBAX 300SB-C8 column.

[0028] (2) Solution preparation: Prepare a mixed solution of BisTris, guanidine hydrochloride, sodium citrate and DTT as working solution I, and prepare guanidine hydrochloride solution as working solution II; the concentration of BisTris in working solution I is 0.1 mol / L, and the concentration of guanidine hydrochloride is 0.1 mol / L. The concentration is 6mol / L, the concentration of sodium citrate is 5.37mmol / L, the concentration of DTT ...

Example Embodiment

[0032] Example 2

[0033] A detection method for identifying adulteration in buffalo milk includes the following steps:

[0034] (1) Sample collection and instrument preparation: Collect buffalo milk and milk samples to be tested and store them in refrigeration. At the same time, prepare the HPLC automatic sampling chromatograph and its matching chromatographic columns and clean them for later use; the milk to be tested is mixed with Holstein milk The temperature of cold storage is 4°C, and the chromatographic column is a ZORBAX 300SB-C8 column.

[0035] (2) Solution preparation: Prepare a mixed solution of BisTris, guanidine hydrochloride, sodium citrate and DTT as working solution I, and prepare guanidine hydrochloride solution as working solution II; the concentration of BisTris in working solution I is 0.1 mol / L, and the concentration of guanidine hydrochloride is 0.1 mol / L. The concentration is 3mol / L, the concentration of sodium citrate is 5mmol / L, the concentration of DTT is ...

Example Embodiment

[0039] Example 3

[0040] A detection method for identifying adulteration in buffalo milk includes the following steps:

[0041] (1) Sample collection and instrument preparation: Collect buffalo milk and milk samples to be tested and store them in refrigeration. At the same time, prepare the HPLC automatic sampling chromatograph and its matching chromatographic columns and clean them for later use; the milk to be tested is mixed with Holstein milk The temperature of cold storage is 4°C, and the chromatographic column is a ZORBAX 300SB-C8 column.

[0042] (2) Solution preparation: Prepare a mixed solution of BisTris, guanidine hydrochloride, sodium citrate and DTT as working solution I, and prepare guanidine hydrochloride solution as working solution II; the concentration of BisTris in working solution I is 0.3 mol / L, the concentration of guanidine hydrochloride The concentration is 8mol / L, the concentration of sodium citrate is 7mmol / L, the concentration of DTT is 25mmol / L, the guan...

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Abstract

The present invention discloses a detection method for identifying an adulterant in buffalo milk, comprising the following steps of firstly performing sample collection and instrument preparation, preparing a mixed solution of BisTris, GuHCI, sodium citrate and DTT as working solution I and preparing GuHCI solution as working solution II; then respectively taking the buffalo milk and the milk sample to be detected at a proper amount and performing the following operations of adding working solution I of equal volume inside, centrifuging them after being vortex mixed and skimming off grease atan upper layer, placing solution at a bottom layer in a new centrifuge tube and adding working solution II inside to be mixed and filtered via a filter membrane; and then placing the mixture inside ahigh performance liquid phase automatic sample-in chromatograph for measurement, thereby obtaining a fingerprint; and performing comparative analysis on the fingerprint. The present invention providesa detection method for rapidly detecting whether the buffalo milk is adulterated with Holstein milk. The present method has good reproducibility and specifity, thereby obtaining a representative fingerprint between samples and analyzing whether the buffalo milk is adulterated with the Holstein milk rapidly and stably.

Description

technical field [0001] The invention relates to the technical field of food detection, in particular to a detection method for identifying adulteration in buffalo milk. Background technique [0002] At present, the buffalo milk on the market varies from good to bad, and there is a phenomenon that ordinary cow milk is used as buffalo milk. Buffalo milk is rich in nutrients. Studies have shown that, except for lactose, the total solids, fat, protein, and ash are higher than milk and human milk [1], especially protein, with an average content of about 4.5%, which is about 1.5 times that of human breast milk and 2.8 times that of human breast milk. It is known as the "king of milk" and is known as "Chinese cheese". Studies have shown that buffalo milk has a variety of active peptides, which have the functions of antibacterial and anti-inflammatory, enhancing immunity, anti-oxidation, anti-aging, lowering blood pressure and anti-cancer. In the market, the price of buffalo milk ...

Claims

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Application Information

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IPC IPC(8): G01N30/06G01N30/86
CPCG01N30/06G01N30/8686
Inventor 曾庆坤梁政洋黄丽李玲黄子珍杨攀
Owner GUANGXI ZHUANG AUTONOMOUS REGION BUFFALO INST
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